Reviewed, UniProtKB/Swiss-Prot Q1QBB6 (ASPD_PSYCK)

Names and origin

Protein names

Recommended name:
    Probable L-aspartate dehydrogenase
    EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	Pcryo_1256

Organism

Psychrobacter cryohalolentis (strain K5) [Complete proteome] [HAMAP]

Taxonomic identifier

335284 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Psychrobacter

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP MF_01265
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP MF_01265
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: HAMAP NADP or NADPH binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_1000067311
Sites
	Active site	216	1	By similarity
	Binding site	120	1	NAD; via amide nitrogen By similarity
	Binding site	186	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1QBB6-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 47927F6132E01CA0	FASTA	263	27,858
	10 20 30 40 50 60 MKNVMFIGYG SMARKVHEML PKNIILSTVL VSTRSAEIIK TELGESIAVI TSVDDLIETP 70 80 90 100 110 120 DLAVEMSGQD GLKEHAIKIL GKSIPLGIIS VGAFTDEKFA ISLADTAEAN GVEIHILAGA 130 140 150 160 170 180 VAGIDGIHAA SFAGLSDVVY QGKKHPSSWK GSHADRLIDY DNLVEPTVFF TGTAREAAAL 190 200 210 220 230 240 FPDNSNVAAT IAIAGVGLDD TTVELIADPT LEYNIHHIMA KGVFGKLEIS MAGLPLVENP 250 260 KTSSLAAFSA LRLCCQIDQV IQM

References

[1]

"Complete sequence of chromosome of Psychrobacter cryohalolentis K5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Sims D.R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000323 Genomic DNA. Translation: ABE75037.1.
RefSeq	YP_580521.1.
3D structure databases
SMR	Q1QBB6. Positions 4-262.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1QBB6.
Genome annotation databases
GeneID	4034285.
GenomeReviews	Gene locus Pcryo_1256 in contig CP000323_GR.
KEGG	pcr:Pcryo_1256.
NMPDR	fig|335284.3.peg.838.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1712.
HOGENOM	HBG649642.
OMA	ECAGHSA.
Enzyme and pathway databases
BioCyc	PCRY335284:PCRYO_1256-MONOMER.
Family and domain databases
HAMAP	MF_01265. NadX. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR020626. Asp_DH_NAD_syn_prok. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASPD_PSYCK

Accession

Primary (citable) accession number: Q1QBB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 16, 2006
Last modified:	January 19, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)