Reviewed, UniProtKB/Swiss-Prot Q1Q9E4 (HUTH_PSYCK)

Names and origin

Protein names

Recommended name:
    Histidine ammonia-lyase
      Short name=Histidase
    EC=4.3.1.3

Gene names

Name:	hutH
Ordered Locus Names:	Pcryo_1932

Organism

Psychrobacter cryohalolentis (strain K5) [Complete proteome] [HAMAP]

Taxonomic identifier

335284 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Psychrobacter

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-histidine = urocanate + NH3. HAMAP MF_00229
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3. HAMAP MF_00229
Subcellular location	Cytoplasm Potential.
Post-translational modification	Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly By similarity.
Sequence similarities	Belongs to the PAL/histidase family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro histidine catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ammonia ligase activity Inferred from electronic annotation. Source: InterPro histidine ammonia-lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Histidine ammonia-lyase HAMAP MF_00229		PRO_1000021567
Amino acid modifications
	Modified residue	147	1	2,3-didehydroalanine (Ser) By similarity
	Cross-link	146 ↔ 148		5-imidazolinone (Ala-Gly) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1Q9E4-1 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 1FD92E53A3EEF57E	FASTA	520	55,756
	10 20 30 40 50 60 MTAIKKLTLQ PRQLSFEILR DIYEQPVILT LPDSAYEAID ASHEDVQTII RRDKSAYGIN 70 80 90 100 110 120 TGFGLLAKTR ISDDQLELLQ RNLIVSHSVG TGEALPANVV RLIMVMKVTS LAQGVSGVRR 130 140 150 160 170 180 AVVDSLLGLI NNQITPNIPA KGSVGASGDL APLSHMTLAM MGEGHVYVDG NKVLAADALA 190 200 210 220 230 240 QHGLEPITLA AKEGLALING TQVSTALALR GYFLARDLLE TATIVGSLSI DAAKGSDAPF 250 260 270 280 290 300 DARIHEVRGH HGQIEIAKAH RQLIKGSAIR ASHDGEQDDR VQDPYCLRCQ PQVMGACLDI 310 320 330 340 350 360 INQAGKTLLI ESNAVTDNPL IFNNEDGPVA ISGGNFHAEP VAFAADILAL AIAEIGSMSE 370 380 390 400 410 420 RRIALLIDAT LSGGLPPFLV DNAGVNSGFM IAHVTAAALA SENKSIAHPG SVDSIPTSAN 430 440 450 460 470 480 QEDHVSMATY CARRLYEMAQ NTATIIGIEL LAAGQGIDFH RGLETSEPLT MAHQKLREQV 490 500 510 520 TFYDKDRYLA PDIEAAKQLV LDGTLAEHWQ PLRSNWYDSK

References

[1]

"Complete sequence of chromosome of Psychrobacter cryohalolentis K5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Sims D.R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000323 Genomic DNA. Translation: ABE75709.1.
RefSeq	YP_581193.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4034669.
GenomeReviews	Gene locus Pcryo_1932 in contig CP000323_GR.
KEGG	pcr:Pcryo_1932.
NMPDR	fig|335284.3.peg.2412.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1Q9E4.
OMA	Q1Q9E4. FAPDIEA.
Enzyme and pathway databases
BioCyc	PCRY335284:PCRYO_1932-MON.
Family and domain databases
HAMAP	MF_00229. [Tree]
InterPro	IPR005921. HutH. IPR001106. Phe/His_NH3-lyase. [Graphical view]
Pfam	PF00221. PAL. 1 hit. [Graphical view]
TIGRFAMs	TIGR01225. hutH. 1 hit.
PROSITE	PS00488. PAL_HISTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HUTH_PSYCK

Accession

Primary (citable) accession number: Q1Q9E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)