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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Psychrobacter cryohalolentis (strain K5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei105S-adenosyl-L-methionineUniRule annotation1
Binding sitei130S-adenosyl-L-methionineUniRule annotation1
Binding sitei157S-adenosyl-L-methionineUniRule annotation1
Active sitei179By similarity1
Binding sitei179S-adenosyl-L-methionineUniRule annotation1
Binding sitei183SubstrateUniRule annotation1
Binding sitei215SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Pcryo_2013
OrganismiPsychrobacter cryohalolentis (strain K5)
Taxonomic identifieri335284 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
Proteomesi
  • UP000002425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002882051 – 273tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST273

Interactioni

Protein-protein interaction databases

STRINGi335284.Pcryo_2013.

Structurei

3D structure databases

SMRiQ1Q963.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni252 – 255Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073968.
KOiK03439.
OMAiGHALEYC.
OrthoDBiPOG091H015P.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1Q963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQHPDINTN VDATSLTDDQ KSLDTNATSG NEVAPDGNKH IRIVNTFMKR
60 70 80 90 100
RTHMNKNAEL ALTAPEFAHY LVNNSFGDGN LEGIDDLRAL FAELPNGSEA
110 120 130 140 150
PLTLEIGFGL GDSFIEMAAA EPSRNFVGIE VHEPGIGKCA YMAGTQNLSN
160 170 180 190 200
VRIINGDAIQ LLKQLPENHI DRIQLYFPDP WQKKRHHKRR FVSPERMAIV
210 220 230 240 250
TRSLKQGGWF HTATDWEHYA FWMVEVLDGF VGLSNQAGAG NFTSRPDFRP
260 270
MTKFERRGLA SGHGVWDLIY LKD
Length:273
Mass (Da):30,592
Last modified:May 16, 2006 - v1
Checksum:iC58090BD003084E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000323 Genomic DNA. Translation: ABE75790.1.
RefSeqiWP_011514330.1. NC_007969.1.

Genome annotation databases

EnsemblBacteriaiABE75790; ABE75790; Pcryo_2013.
KEGGipcr:Pcryo_2013.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTRMB_PSYCK
AccessioniPrimary (citable) accession number: Q1Q963
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 16, 2006
Last modified: June 7, 2017
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families