SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q1Q8S6

- BIOB_PSYCK

UniProt

Q1Q8S6 - BIOB_PSYCK

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Biotin synthase
Gene
bioB, Pcryo_2150
Organism
Psychrobacter cryohalolentis (strain K5)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi83 – 831Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi87 – 871Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi90 – 901Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi129 – 1291Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi160 – 1601Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi220 – 2201Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi296 – 2961Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPCRY335284:GHE9-2193-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Pcryo_2150
OrganismiPsychrobacter cryohalolentis (strain K5)
Taxonomic identifieri335284 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
ProteomesiUP000002425: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Biotin synthaseUniRule annotation
PRO_0000381569Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi335284.Pcryo_2150.

Structurei

3D structure databases

ProteinModelPortaliQ1Q8S6.
SMRiQ1Q8S6. Positions 36-349.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1Q8S6-1 [UniParc]FASTAAdd to Basket

« Hide

MSALLNITEP NNNTNSNDND FLASLTTDAP QAIASKYSRE QIAQLFDLPL    50
MDLLLQAQTI HRQNFNANEV QISTLLSIKT GNCPEDCGYC SQSGHHRDKT 100
KLVAEKRIEV DKVIAAAKRA KATGSSRFCM GAAWKHPSAK DMPYVVELVK 150
EVKALGLETC MTLGMLDTDQ AAQLADAGLD YYNHNLDTSR SYYEQVVSTR 200
SYDERLDTIT NVRNSGINVC SGNIVGMGES RDDRIDWVHE LLKMPKAPES 250
IPVNLLVPIQ GTPIGDKVLA EGQLSVLEWI RTIAVTRICC PSSYVRLSAG 300
RESLSDAEQA LAFMAGANSF FYGDKLLTTG NASQSGDDRL MRELGLTKQF 350
AAPRAPKQVP VIDAMSGHQS QVVLAS 376
Length:376
Mass (Da):41,118
Last modified:May 16, 2006 - v1
Checksum:i1AF4D78809EF4111
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000323 Genomic DNA. Translation: ABE75927.1.
RefSeqiYP_581411.1. NC_007969.1.

Genome annotation databases

EnsemblBacteriaiABE75927; ABE75927; Pcryo_2150.
GeneIDi4033725.
KEGGipcr:Pcryo_2150.
PATRICi23063927. VBIPsyCry128170_2277.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000323 Genomic DNA. Translation: ABE75927.1 .
RefSeqi YP_581411.1. NC_007969.1.

3D structure databases

ProteinModelPortali Q1Q8S6.
SMRi Q1Q8S6. Positions 36-349.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 335284.Pcryo_2150.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE75927 ; ABE75927 ; Pcryo_2150 .
GeneIDi 4033725.
KEGGi pcr:Pcryo_2150.
PATRICi 23063927. VBIPsyCry128170_2277.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci PCRY335284:GHE9-2193-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Psychrobacter cryohalolentis K5."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Sims D.R., Gilna P., Schmutz J.
    , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Richardson P.
    Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K5.

Entry informationi

Entry nameiBIOB_PSYCK
AccessioniPrimary (citable) accession number: Q1Q8S6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 16, 2006
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi