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Protein

Biotin synthase

Gene

bioB

Organism
Psychrobacter cryohalolentis (strain K5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi83Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi87Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi90Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi129Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi160Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi220Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi296Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Pcryo_2150
OrganismiPsychrobacter cryohalolentis (strain K5)
Taxonomic identifieri335284 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
Proteomesi
  • UP000002425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003815691 – 376Biotin synthaseAdd BLAST376

Proteomic databases

PRIDEiQ1Q8S6

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi335284.Pcryo_2150

Structurei

3D structure databases

ProteinModelPortaliQ1Q8S6
SMRiQ1Q8S6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF Bacteria
COG0502 LUCA
HOGENOMiHOG000239957
KOiK01012
OMAiADRFCMG
OrthoDBiPOG091H01DF

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDF00272 biotin_synthase, 1 hit
SFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

Q1Q8S6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALLNITEP NNNTNSNDND FLASLTTDAP QAIASKYSRE QIAQLFDLPL
60 70 80 90 100
MDLLLQAQTI HRQNFNANEV QISTLLSIKT GNCPEDCGYC SQSGHHRDKT
110 120 130 140 150
KLVAEKRIEV DKVIAAAKRA KATGSSRFCM GAAWKHPSAK DMPYVVELVK
160 170 180 190 200
EVKALGLETC MTLGMLDTDQ AAQLADAGLD YYNHNLDTSR SYYEQVVSTR
210 220 230 240 250
SYDERLDTIT NVRNSGINVC SGNIVGMGES RDDRIDWVHE LLKMPKAPES
260 270 280 290 300
IPVNLLVPIQ GTPIGDKVLA EGQLSVLEWI RTIAVTRICC PSSYVRLSAG
310 320 330 340 350
RESLSDAEQA LAFMAGANSF FYGDKLLTTG NASQSGDDRL MRELGLTKQF
360 370
AAPRAPKQVP VIDAMSGHQS QVVLAS
Length:376
Mass (Da):41,118
Last modified:May 16, 2006 - v1
Checksum:i1AF4D78809EF4111
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000323 Genomic DNA Translation: ABE75927.1
RefSeqiWP_011514463.1, NC_007969.1

Genome annotation databases

EnsemblBacteriaiABE75927; ABE75927; Pcryo_2150
KEGGipcr:Pcryo_2150

Similar proteinsi

Entry informationi

Entry nameiBIOB_PSYCK
AccessioniPrimary (citable) accession number: Q1Q8S6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 16, 2006
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health