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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadB

Organism
Psychrobacter cryohalolentis (strain K5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.UniRule annotation

Catalytic activityi

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation
A (3Z)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.UniRule annotation
A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei120Important for catalytic activityUniRule annotation1
Sitei140Important for catalytic activityUniRule annotation1
Binding sitei298SubstrateUniRule annotation1
Binding sitei326NAD; via amide nitrogenUniRule annotation1
Binding sitei345NADUniRule annotation1
Binding sitei409NADUniRule annotation1
Binding sitei431NADUniRule annotation1
Active sitei452For 3-hydroxyacyl-CoA dehydrogenase activityUniRule annotation1
Binding sitei455NADUniRule annotation1
Binding sitei502SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi402 – 404NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation, EC:5.3.3.8UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadBUniRule annotation
Ordered Locus Names:Pcryo_2227
OrganismiPsychrobacter cryohalolentis (strain K5)
Taxonomic identifieri335284 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
Proteomesi
  • UP000002425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002558441 – 719Fatty acid oxidation complex subunit alphaAdd BLAST719

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).UniRule annotation

Protein-protein interaction databases

STRINGi335284.Pcryo_2227

Structurei

3D structure databases

ProteinModelPortaliQ1Q8J9
SMRiQ1Q8J9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 190Enoyl-CoA hydratase/isomeraseUniRule annotationAdd BLAST190
Regioni313 – 7193-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST407

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DYT Bacteria
COG1250 LUCA
HOGENOMiHOG000261344
KOiK01825
OMAiIDLCMIL
OrthoDBiPOG091H00UW

Family and domain databases

HAMAPiMF_01621 FadB, 1 hit
InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR001753 Enoyl-CoA_hydra/iso
IPR012799 FadB
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 1 hit
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02437 FadB, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit

Sequencei

Sequence statusi: Complete.

Q1Q8J9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYQGNRITV TMLEDGIANM QYNAENESVN KFDTETNKQF AEVVNALEKA
60 70 80 90 100
DDIKGLIVTS SKGVFIAGAD ITEFVASFKQ SEEEIKDWVI NINDAFNRFE
110 120 130 140 150
DLPFPKVAAI NGAALGGGCE MTLVCEYRVM SDKAIIGLPE TQLGIFPGFG
160 170 180 190 200
GTVRSTRVIG IDNALELIAT GTPKKALDAL KLGLVDATVA ADDLQDAAID
210 220 230 240 250
LVKKCISDEL DWQAKREEKL VPVKLNQLEQ AMAFNSAKGM IFAKANPKQY
260 270 280 290 300
PAPALAIAAI EKHVNLPRDK AIEVEAAGFA KAAKTPQAES LVGLFLSDQL
310 320 330 340 350
VKKLAKQHSK KAHDINEAAV LGAGIMGGGI AYQAASKGLP IIMKDIKSEQ
360 370 380 390 400
LDLGMGEASK LLGKMVDRGK ITPAKMGETL SRIRPTLNYG DFAETDIVIE
410 420 430 440 450
AVVENPNVKR AVLKEVEGLV KDDCILASNT STISITFLAE ALERPENFVG
460 470 480 490 500
MHFFNPVNRM PLVEVIRGEK SSEEAISTTV ALATKMGKVP VVVNDCPGFL
510 520 530 540 550
VNRVLFPYFG AFDLLLKQGA DFAHVDKVME KFGWPMGPAY LIDVVGLDTG
560 570 580 590 600
VHGAEVMAEG FPDRMKPDYK GAIEHLYENK RLGQKNGVGF YKYEMDKRGK
610 620 630 640 650
PKKVADEATY ELLKTTTDSD KQTFEDQAII DRTMLAFCNE TVRCLEDNIV
660 670 680 690 700
STPSEADMAM IMGVGFPPFR GGPCRYIDQM GLDNYLALCE KYAYLGKAYE
710
APQKIRDMAA AGETFYATA
Length:719
Mass (Da):78,378
Last modified:May 16, 2006 - v1
Checksum:i0A15859CAFCD9966
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000323 Genomic DNA Translation: ABE76004.1
RefSeqiWP_011514537.1, NC_007969.1

Genome annotation databases

EnsemblBacteriaiABE76004; ABE76004; Pcryo_2227
KEGGipcr:Pcryo_2227

Similar proteinsi

Entry informationi

Entry nameiFADB_PSYCK
AccessioniPrimary (citable) accession number: Q1Q8J9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: May 16, 2006
Last modified: April 25, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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