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Protein

Thymidylate synthase

Gene

thyA

Organism
Psychrobacter cryohalolentis (strain K5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31dUMPUniRule annotation1
Binding sitei615,10-methylenetetrahydrofolateUniRule annotation1
Active sitei172NucleophileUniRule annotation1
Binding sitei1955,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei203dUMPUniRule annotation1
Binding sitei2895,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi152 – 153dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi192 – 195dUMPUniRule annotation4
Nucleotide bindingi233 – 235dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:Pcryo_2314
OrganismiPsychrobacter cryohalolentis (strain K5)
Taxonomic identifieri335284 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
Proteomesi
  • UP000002425 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003214751 – 290Thymidylate synthaseAdd BLAST290

Proteomic databases

PRIDEiQ1Q8B2

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi335284.Pcryo_2314

Structurei

3D structure databases

ProteinModelPortaliQ1Q8B2
SMRiQ1Q8B2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA
HOGENOMiHOG000257899
KOiK00560
OMAiKQYLDLC
OrthoDBiPOG091H02L6

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q1Q8B2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMNSSIIKN EQAYLDLLRL VLNEGTEKGD RTGTGTLSHF GAQLRFNLAD
60 70 80 90 100
GFPLLTTKKV HLKSITYELL WFLNGSTHVD YLQQNGVRIW NEWATSEQTA
110 120 130 140 150
RFNRPAGDLG PIYGHQWRNY GATTTADGQY NSDGVDQIAQ VVEQIKTNPN
160 170 180 190 200
SRRLIVSGWN PGEADQVALP PCHTLFQFFV ADNKLSCQLY QRSADLFLGV
210 220 230 240 250
PFNIASYAML THMVAQVCNL EVGEFIWTGG DCHIYQNHRE QVELQLTRSL
260 270 280 290
YTLPTLALNP NVKDIFAFNY EDISVDGYES HPAIKAQVAV
Length:290
Mass (Da):32,621
Last modified:May 16, 2006 - v1
Checksum:iBD3EA5E53368AA8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000323 Genomic DNA Translation: ABE76091.1
RefSeqiWP_011514621.1, NC_007969.1

Genome annotation databases

EnsemblBacteriaiABE76091; ABE76091; Pcryo_2314
KEGGipcr:Pcryo_2314

Similar proteinsi

Entry informationi

Entry nameiTYSY_PSYCK
AccessioniPrimary (citable) accession number: Q1Q8B2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 16, 2006
Last modified: May 23, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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