Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Psychrobacter cryohalolentis (strain K5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi117ZincUniRule annotation1
Metal bindingi119ZincUniRule annotation1
Metal bindingi144ZincUniRule annotation1
Metal bindingi146ZincUniRule annotation1
Binding sitei264ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Pcryo_2381
OrganismiPsychrobacter cryohalolentis (strain K5)
Taxonomic identifieri335284 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
Proteomesi
  • UP000002425 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000019431 – 509Glutamate--tRNA ligaseAdd BLAST509

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi335284.Pcryo_2381

Structurei

3D structure databases

ProteinModelPortaliQ1Q845
SMRiQ1Q845
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi20 – 30"HIGH" regionUniRule annotationAdd BLAST11
Motifi261 – 265"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q1Q845-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMQPSTSTNS IRPVRTRIAP SPTGFPHVGT AYIALFNLAF AKAHGGEFIL
60 70 80 90 100
RIEDTDQTRS TEQSEKMILD ALRWVGLDWA EGPDIGGPHA PYRQSERSDI
110 120 130 140 150
YKKHAEQLIE NDHAFRCFCS SEELDAMRAE QMANGETPRY DGRCAHLAAE
160 170 180 190 200
KTEQLVAEGK PHVIRMRVPT EGVCQVQDML RGTVEIPWTQ VDMQVLLKTD
210 220 230 240 250
GMPTYHLANV VDDHLMDISH VMRGEEWLNS APKHQLLYEY FGWEMPVLCH
260 270 280 290 300
MPLLRNPDKS KLSKRKNPTS ITYYRDAGVL PEALLNYLGR MGYSMPDEAE
310 320 330 340 350
QFTLEEMIAS FDIQRVSLGG PIFDIEKLNW LNSEWLRALT PEELKNKILE
360 370 380 390 400
WASNSDKLTA IAAAIQPRIE LLSDAVNWSG FYFQNLPDIN AESFTHKSLT
410 420 430 440 450
PEQIMDMLQL SLWQLEVLPT WSEENIYATL KGLAAHLDIK MRDFMAPFFI
460 470 480 490 500
AIAGSTSSTP VMNSMAIIGA DMTLTRLRHA VDVLGGLGKK KLKKLEKQAA

ELPDFLAAE
Length:509
Mass (Da):57,493
Last modified:May 16, 2006 - v1
Checksum:iC5D4C77FD311B544
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000323 Genomic DNA Translation: ABE76158.1
RefSeqiWP_011514686.1, NC_007969.1

Genome annotation databases

EnsemblBacteriaiABE76158; ABE76158; Pcryo_2381
KEGGipcr:Pcryo_2381

Similar proteinsi

Entry informationi

Entry nameiSYE_PSYCK
AccessioniPrimary (citable) accession number: Q1Q845
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 16, 2006
Last modified: March 28, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health