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Protein

E3 ubiquitin-protein ligase TRIM71

Gene

Trim71

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1.3 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri12 – 9483RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri181 – 22848B box-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri260 – 30142B box-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • miRNA binding Source: UniProtKB
  • translation repressor activity Source: MGI
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • 3'-UTR-mediated mRNA destabilization Source: MGI
  • cellular response to organic substance Source: MGI
  • fibroblast growth factor receptor signaling pathway Source: UniProtKB
  • G1/S transition of mitotic cell cycle Source: UniProtKB
  • miRNA mediated inhibition of translation Source: UniProtKB
  • miRNA metabolic process Source: UniProtKB
  • negative regulation of translation Source: MGI
  • neural tube closure Source: MGI
  • neural tube development Source: UniProtKB
  • positive regulation of gene silencing by miRNA Source: MGI
  • protein autoubiquitination Source: UniProtKB
  • regulation of gene silencing by miRNA Source: UniProtKB
  • regulation of neural precursor cell proliferation Source: UniProtKB
  • regulation of protein metabolic process Source: MGI
  • stem cell proliferation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Ligase

Keywords - Biological processi

RNA-mediated gene silencing, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM71 (EC:6.3.2.-)
Alternative name(s):
Protein lin-41 homolog
Short name:
mLin41
Tripartite motif-containing protein 71
Gene namesi
Name:Trim71
Synonyms:Gm1127, Lin41
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2685973. Trim71.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic mRNA processing body Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality, possibly due to a neural tube closure defect during development. Reduced cell proliferation in the neuroepithelium.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi12 – 121C → L: Abolishes E3 ubiquitin-protein ligase activity; when associated with A-15. 1 Publication
Mutagenesisi15 – 151C → A: Abolishes E3 ubiquitin-protein ligase activity; when associated with L-12. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 855854E3 ubiquitin-protein ligase TRIM71PRO_0000279512Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Post-translational modificationi

Autoubiquitinated.1 Publication

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

PaxDbiQ1PSW8.
PeptideAtlasiQ1PSW8.
PRIDEiQ1PSW8.

PTM databases

iPTMnetiQ1PSW8.
PhosphoSiteiQ1PSW8.

Expressioni

Tissue specificityi

Highly expressed in undifferentiated embryonic stem cells (ESCs). Expressed in the epiblast and in interfollicular epidermal stem cells during early development. Also expressed in male germ cells and in the reproductive tract. Highly expressed in neuroepithelial cells, and its expression declines as neurogenesis proceeds (at protein level).3 Publications

Developmental stagei

Expression is first detected at E8.5, increases to highest levels at E14.5 and remains elevated through the newborn stage. Expressed in developing limb buds and tail buds starting from E9.5.2 Publications

Inductioni

Negatively regulated by the microRNA (miRNA) let-7 which causes degradation of the mRNA encoding this protein. This requires a let-7 complementary site (LCS) in the 3'-UTR of the mRNA encoding this protein.1 Publication

Gene expression databases

BgeeiENSMUSG00000079259.
CleanExiMM_TRIM71.
GenevisibleiQ1PSW8. MM.

Interactioni

Subunit structurei

Interacts (via NHL repeats) with AGO2. Interacts with SHCBP1; leading to enhance its stability.3 Publications

Protein-protein interaction databases

BioGridi561929. 11 interactions.
STRINGi10090.ENSMUSP00000107447.

Structurei

3D structure databases

ProteinModelPortaliQ1PSW8.
SMRiQ1PSW8. Positions 476-565, 583-855.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati466 – 567102FilaminAdd
BLAST
Repeati580 – 62344NHL 1Add
BLAST
Repeati627 – 67044NHL 2Add
BLAST
Repeati674 – 71744NHL 3Add
BLAST
Repeati721 – 76444NHL 4Add
BLAST
Repeati768 – 81144NHL 5Add
BLAST
Repeati815 – 85541NHL 6Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili314 – 35239Sequence analysisAdd
BLAST
Coiled coili378 – 41134Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi26 – 4217Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 2 B box-type zinc fingers.PROSITE-ProRule annotation
Contains 1 filamin repeat.PROSITE-ProRule annotation
Contains 6 NHL repeats.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri12 – 9483RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri181 – 22848B box-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri260 – 30142B box-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITG8. Eukaryota.
ENOG410XSQC. LUCA.
GeneTreeiENSGT00550000074377.
HOGENOMiHOG000006755.
HOVERGENiHBG081916.
InParanoidiQ1PSW8.
KOiK12035.
OMAiMASFPET.
OrthoDBiEOG091G02WU.
PhylomeDBiQ1PSW8.
TreeFamiTF331018.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
2.60.40.10. 1 hit.
3.30.40.10. 2 hits.
4.10.45.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR017868. Filamin/ABP280_repeat-like.
IPR001298. Filamin/ABP280_rpt.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00630. Filamin. 1 hit.
PF01436. NHL. 6 hits.
PF00643. zf-B_box. 1 hit.
[Graphical view]
SMARTiSM00336. BBOX. 2 hits.
SM00557. IG_FLMN. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiPS50194. FILAMIN_REPEAT. 1 hit.
PS51125. NHL. 6 hits.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q1PSW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASFPETDFQ ICLLCKEMCG SPAPLSSNSS ASSSSSQTST SSAGGGGPGA
60 70 80 90 100
AARRLHVLPC LHAFCRPCLE AHRLPAPGGA GPAEALKLRC PVCDQKVVLA
110 120 130 140 150
EAAGMDALPS SAFLLSNLLD AVVATAEEPP PKNGRAGGGP GGAGGHSNHR
160 170 180 190 200
HHAHHPAQRA AAPAPQPPPG PAASPGSLLM RRPHGCSSCD EGNAASSRCL
210 220 230 240 250
DCQEHLCDNC VRAHQRVRLT KDHYIERGPP GPAAASAAQQ LGLGPPFAGA
260 270 280 290 300
PFSILSVFPE RLGFCQHHDD EVLHLYCDTC SVPICRECTL GRHGGHSFAY
310 320 330 340 350
LQDALQDSRA LTIQLLADAQ QGRQALQLSI EQAQTVAEQV EMKAKVVQSE
360 370 380 390 400
VKAVTARHKK ALEDRECELL WKVEKIRQVK AKSLFLQVEK LRQSLSKLES
410 420 430 440 450
TISAVQQVLE EGRALDILLA RDRMLAQVQE LKTIRGLLQP QEDDRIMFTP
460 470 480 490 500
PDQALYLALK SIGFVSSGAF APLTKATGDG IKRALQGKVA SFTVMGYDHD
510 520 530 540 550
GEPRHSGGDL MSVVVLGPDG NLFGAEVSDQ QNGTYIVSYR PQLEGEHLVS
560 570 580 590 600
VTLYNQHIEN SPFKVVVKSG RSYVGIGLPG LSFGSEGDGE GKLCRPWGVS
610 620 630 640 650
VDKEGFIIVA DRSNNRIQVF KPCGSFHHKF GTLGSRPGQF DRPAGVACDA
660 670 680 690 700
SRRIIVADKD NHRIQIFTFE GQFLLKFGEK GTKNGQFNYP WDVAVNSEGK
710 720 730 740 750
ILVSDTRNHR IQLFGPDGVF LNKYGFEGSL WKHFDSPRGV AFNNEGHLVV
760 770 780 790 800
TDFNNHRLLV IHPDCQSARF LGSEGSGNGQ FLRPQGVAVD QEGRIIVADS
810 820 830 840 850
RNHRVQMFEA NGSFLCKFGA QGSGFGQMDR PSGIAVTPDG LIVVVDFGNN

RILIF
Length:855
Mass (Da):92,054
Last modified:May 16, 2006 - v1
Checksum:i151BA48E28C34904
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ005956 mRNA. Translation: AAY55947.1.
CCDSiCCDS40793.1.
RefSeqiNP_001035968.1. NM_001042503.2.
UniGeneiMm.17857.

Genome annotation databases

EnsembliENSMUST00000111816; ENSMUSP00000107447; ENSMUSG00000079259.
GeneIDi636931.
KEGGimmu:636931.
UCSCiuc009rxp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ005956 mRNA. Translation: AAY55947.1.
CCDSiCCDS40793.1.
RefSeqiNP_001035968.1. NM_001042503.2.
UniGeneiMm.17857.

3D structure databases

ProteinModelPortaliQ1PSW8.
SMRiQ1PSW8. Positions 476-565, 583-855.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi561929. 11 interactions.
STRINGi10090.ENSMUSP00000107447.

PTM databases

iPTMnetiQ1PSW8.
PhosphoSiteiQ1PSW8.

Proteomic databases

PaxDbiQ1PSW8.
PeptideAtlasiQ1PSW8.
PRIDEiQ1PSW8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000111816; ENSMUSP00000107447; ENSMUSG00000079259.
GeneIDi636931.
KEGGimmu:636931.
UCSCiuc009rxp.1. mouse.

Organism-specific databases

CTDi131405.
MGIiMGI:2685973. Trim71.

Phylogenomic databases

eggNOGiENOG410ITG8. Eukaryota.
ENOG410XSQC. LUCA.
GeneTreeiENSGT00550000074377.
HOGENOMiHOG000006755.
HOVERGENiHBG081916.
InParanoidiQ1PSW8.
KOiK12035.
OMAiMASFPET.
OrthoDBiEOG091G02WU.
PhylomeDBiQ1PSW8.
TreeFamiTF331018.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ1PSW8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000079259.
CleanExiMM_TRIM71.
GenevisibleiQ1PSW8. MM.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
2.60.40.10. 1 hit.
3.30.40.10. 2 hits.
4.10.45.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR017868. Filamin/ABP280_repeat-like.
IPR001298. Filamin/ABP280_rpt.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00630. Filamin. 1 hit.
PF01436. NHL. 6 hits.
PF00643. zf-B_box. 1 hit.
[Graphical view]
SMARTiSM00336. BBOX. 2 hits.
SM00557. IG_FLMN. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiPS50194. FILAMIN_REPEAT. 1 hit.
PS51125. NHL. 6 hits.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLIN41_MOUSE
AccessioniPrimary (citable) accession number: Q1PSW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: May 16, 2006
Last modified: September 7, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Reported to mediate ubiquitination and subsequent degradation of AGO2 (PubMed:19898466). However, this result is subject to discussion and later reports suggest that, while it interacts with AGO2, it is not involved in AGO2 ubiquitination (PubMed:22508726, PubMed:22735451).3 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.