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Protein

Glutamate 5-kinase

Gene

proB

Organism
uncultured Prochlorococcus marinus clone HF10-88D1
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotationSAAS annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. no protein annotated in this organism
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei7ATPUniRule annotation1
Binding sitei47SubstrateUniRule annotation1
Binding sitei134SubstrateUniRule annotation1
Binding sitei146Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 167ATPUniRule annotation2
Nucleotide bindingi210 – 216ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationSAAS annotationImported, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotationImported
ORF Names:HF10-88D1_0034Imported
Organismiuncultured Prochlorococcus marinus clone HF10-88D1Imported
Taxonomic identifieri379377 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesProchloraceaeProchlorococcus

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliQ1PK02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini275 – 356PUAInterPro annotationAdd BLAST82

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotationSAAS annotation

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiView protein in InterPro
IPR036393. AceGlu_kinase-like_sf.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_sf.
IPR036974. PUA_sf.
PfamiView protein in Pfam
PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiView protein in SMART
SM00359. PUA. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiView protein in PROSITE
PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1PK02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIWVIKIGT SILRGTEETS TEEVIENLSR SFTSFLSKGN KLILVTSGAV
60 70 80 90 100
GLGCQKLNIK TRPNDLSTLQ ATAAVGQVNL MSLYDKVFNK LGHNIAQILI
110 120 130 140 150
TKADFNSRES FNNASKTLKK LIDLNVIPIV NENDTVANEE LKYGDNDTLS
160 170 180 190 200
ALVALAINAN KLILLTDIEN LYSKDPRNNK DAQPIKEVHN SELKEIKDKN
210 220 230 240 250
TQNSNNEWGT GGISTKLISA EIATKGGVEV QLVDGTNKKN LIEIFNDNKI
260 270 280 290 300
GTLFYPVEKP IGNKKSWLSH AIQTVGKITL DDGASFAIKK KGASLLAVGV
310 320 330 340 350
KNVEGNFTIN QAVKIVNTND KEVAKGLVSI SSDKLRSILN NKENNNSSII
360
VVHRDVLALS
Length:360
Mass (Da):39,238
Last modified:May 16, 2006 - v1
Checksum:iD849CC8C2BE0ADBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ366725 Genomic DNA. Translation: ABE11228.1.

Similar proteinsi

Entry informationi

Entry nameiQ1PK02_PROMR
AccessioniPrimary (citable) accession number: Q1PK02
Entry historyiIntegrated into UniProtKB/TrEMBL: May 16, 2006
Last sequence update: May 16, 2006
Last modified: November 22, 2017
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)