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Q1PHW8

- Q1PHW8_9ENTO

UniProt

Q1PHW8 - Q1PHW8_9ENTO

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Protein

Genome polyprotein

Gene
N/A
Organism
Human poliovirus 1
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

NTP + H2O = NDP + phosphate.SAAS annotation
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SAAS annotation
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.SAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1472 – 14721ZincImported
Metal bindingi1479 – 14791Zinc; via tele nitrogenImported
Metal bindingi1481 – 14811Zinc; via pros nitrogenImported
Metal bindingi1490 – 14901ZincImported

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. ion channel activity Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW
  5. RNA binding Source: UniProtKB-KW
  6. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  7. RNA helicase activity Source: InterPro
  8. structural molecule activity Source: InterPro

GO - Biological processi

  1. pore formation by virus in membrane of host cell Source: UniProtKB-KW
  2. protein oligomerization Source: UniProtKB-KW
  3. RNA-protein covalent cross-linking Source: InterPro
  4. transcription, DNA-templated Source: InterPro
  5. viral entry into host cell Source: UniProtKB-KW
  6. viral RNA genome replication Source: InterPro
  7. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

HelicaseSAAS annotation, Hydrolase, Ion channel, Nucleotidyltransferase, Protease, RNA-directed RNA polymeraseSAAS annotation, Thiol proteaseSAAS annotation, Transferase, Viral ion channelSAAS annotation

Keywords - Biological processi

Host-virus interaction, Ion transport, Transport, Viral attachment to host cellSAAS annotation, Viral RNA replicationSAAS annotation, Virus entry into host cell

Keywords - Ligandi

ATP-binding, Metal-bindingImported, Nucleotide-binding, RNA-bindingSAAS annotation, ZincImported

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyproteinSAAS annotation
OrganismiHuman poliovirus 1Imported
Taxonomic identifieri12080 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeEnterovirusEnterovirus C

Subcellular locationi

Host cytoplasmic vesicle membrane SAAS annotation; Peripheral membrane protein SAAS annotation; Cytoplasmic side SAAS annotation

GO - Cellular componenti

  1. host cell cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  2. integral to membrane of host cell Source: UniProtKB-KW
  3. membrane Source: UniProtKB-KW
  4. viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid proteinSAAS annotation, Host cytoplasmSAAS annotation, Host cytoplasmic vesicleSAAS annotation, Host membraneSAAS annotation, Membrane, Virion

PTM / Processingi

Keywords - PTMi

PhosphoproteinSAAS annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IJDX-ray3.401/21027-1670[»]
ProteinModelPortaliQ1PHW8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains RdRp catalytic domain.SAAS annotation
Contains SF3 helicase domain.SAAS annotation
Contains SFhelicase domain.SAAS annotation

Family and domain databases

Gene3Di2.60.120.20. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR014838. P3A.
IPR000081. Peptidase_C3.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR002527. Pico_P2B.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view]
PfamiPF08727. P3A. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF00947. Pico_P2A. 1 hit.
PF01552. Pico_P2B. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
ProDomiPD001306. Peptidase_C3. 1 hit.
PD649346. Pico_P2B. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF89043. SSF89043. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q1PHW8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
VVPLSTPREM DILGFVSACN DFSVRLLRDT THIEQKALAQ GLGQMLESMI
60 70 80 90 100
DNTVRETVGA ATSRDALPNT EASGPAHSKE IPALTAVETG ATNPLVPSDT
110 120 130 140 150
VQTRHVIQHR SRSESSIESF FARGACVTIM TVDNSASTGS KDKLFTVWKI
160 170 180 190 200
TYKDTVQLRR KLEFFTYSRF DMEFTFVVTA NFTETNNGHA LNQVYQIMYV
210 220 230 240 250
PPGAPVPKEW DDYTWQTSSN PSIFYTYGTA PARISIPYVG ISNAYSHFYD
260 270 280 290 300
GFSKIPLKDQ TAALGDSLYG AASLNDFGIL AVRVVNDHNP TKVTSKIRVY
310 320 330 340 350
LKPKHIRVWC PRPPRAVAYY GPGVDYKDGT LTPLPTKDLT TYGFGHQNKA
360 370 380 390 400
VYTAGYKICN YHLATQEDLQ NAVNVMWDRD LLVTESRSQG TDSIARCNCN
410 420 430 440 450
TGVYYCESRR KYYPVSFVGP TFQYMEANDY YPARYQSHML IGHGFASPGD
460 470 480 490 500
CGGILRCRHG VIGIITAGGE GLVAFSDIRD LYAYEEEAME QGITNYIESL
510 520 530 540 550
GAAFGSGFTQ QIGDKITELT NMVTSTITEK LLKNLIKIIS SLVIITRNYE
560 570 580 590 600
DTTTVLATLA LLGCDISPWQ WLKKKACDVL EIPYVIKQGD SWLKKFTEAC
610 620 630 640 650
NAAKGLEWVS NKISKFIDWL KEKIIPQARD KLEFVTKLRQ LEMLENQIST
660 670 680 690 700
IHQSCPSQEH QEILFNNVRW LSIQSKRFAP LYAVEAKRIQ KLEHTINNYI
710 720 730 740 750
QFKSKHRIEP VCLLVHGSPG TGKSVATNLI ARAIAERENT STYSLPPDPS
760 770 780 790 800
HFDGYKQQGV VIMDDLNQNP DGADMKLFCQ MVSTVEFIPP MASLEEKGIL
810 820 830 840 850
FTSNYVLAST NSSRISPPTV AHSDALARRF AFDMDIQVMN EYSRDGKLNM
860 870 880 890 900
AMATEMCKNC HQPANFKRCC PLVCGKAIQL MDKSSRVRYS IDQITTMIIN
910 920 930 940 950
ERNRRSNIGN CMEALFQGPL QYKDLKIDIK TSPPPECIND LLQAVDSQEV
960 970 980 990 1000
RDYCEKKGWI VNITSQVQTE RNINRAMTIL QAVTTFAAVA GVVYVMYKLF
1010 1020 1030 1040 1050
AGHQGAYTGL PNKKPNVPTI RTAKVQGPGF DYAVAMAKRN IVTATTSKGE
1060 1070 1080 1090 1100
FTMLGVHDNV AILPTHASPG ESIVIDGKEV EILDAKALED QAGTNLEITI
1110 1120 1130 1140 1150
ITLKRNEKFR DIRPHIPTQI TETNDGVLIV NTSKYPNMYV PVGAVTEQGY
1160 1170 1180 1190 1200
LNLGGRQTAR TLMYNFPTRA GQCGGVITCT GKVIGMHVGG NGSHGFAAAL
1210 1220 1230 1240 1250
KRSYFTQSQG EIQWMRPSKE VGYPIINAPS KTKLEPSAFH YVFEGVKEPA
1260 1270 1280 1290 1300
VLTKNDPRLK TDFEEAIFSK YVGNKITEVD EYMKEAVDHY AGQLMSLDIN
1310 1320 1330 1340 1350
TEQMCLEDAM YGTDGLEALD LSTSAGYPYV AMGKKKRDIL NKQTRDTKEM
1360 1370 1380 1390 1400
QKLLDTYGIN LPLVTYVKDE LRSKTKVEMG KSRLIEASSL NDSVAMRMAF
1410 1420 1430 1440 1450
GNLYAAFHKN PGVVTGSAVG CDPDLFWSKI PVLMEEKLFA FDYTGYDASL
1460 1470 1480 1490 1500
SPAWFEALKM VLEKIGFGDR VDYIDYLNHS HHLYKNKTYC VKGGMPSGCS
1510 1520 1530 1540 1550
GTSIFNSMIN NLIIRTLLLK TYKGIDLDHL KMIAYGDDVI ASYPHEVDAS
1560 1570 1580 1590 1600
LLAQSGKDYG LTMTPADKSA TFETVTWENV TFLKRFFRAD EKYPFLIHPV
1610 1620 1630 1640 1650
MPMKEIHESI RWTKDPRNTQ DHVRSLCLLA WHNGEEEYNK FLAKIRSVPI
1660 1670
GRALLLPEYS TLYRRWLDSF
Length:1,670
Mass (Da):186,996
Last modified:May 16, 2006 - v1
Checksum:i31D825913C635FCE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11Imported

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ411563 Genomic RNA. Translation: ABE02680.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ411563 Genomic RNA. Translation: ABE02680.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2IJD X-ray 3.40 1/2 1027-1670 [» ]
ProteinModelPortali Q1PHW8.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.20. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR014838. P3A.
IPR000081. Peptidase_C3.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR002527. Pico_P2B.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view ]
Pfami PF08727. P3A. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF00947. Pico_P2A. 1 hit.
PF01552. Pico_P2B. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
ProDomi PD001306. Peptidase_C3. 1 hit.
PD649346. Pico_P2B. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF89043. SSF89043. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genetic drift of poliovirus type 1 during 51 months of prolonged intestinal replication in an immunodeficient patient."
    Vollnberg B., Diedrich S., Schreier E.
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: PV1/00-87/DEUImported.
  2. "Crystal structure of poliovirus 3CD protein: virally encoded protease and precursor to the RNA-dependent RNA polymerase."
    Marcotte L.L., Wass A.B., Gohara D.W., Pathak H.B., Arnold J.J., Filman D.J., Cameron C.E., Hogle J.M.
    J. Virol. 81:3583-3596(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.40 ANGSTROMS) OF 1027-1670 IN COMPLEX WITH ZINC.

Entry informationi

Entry nameiQ1PHW8_9ENTO
AccessioniPrimary (citable) accession number: Q1PHW8
Entry historyi
Integrated into UniProtKB/TrEMBL: May 16, 2006
Last sequence update: May 16, 2006
Last modified: November 26, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3