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Q1PHW8

- Q1PHW8_9ENTO

UniProt

Q1PHW8 - Q1PHW8_9ENTO

Protein
Submitted name:

Polyprotein

Gene
N/A
Organism
Human poliovirus 1
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (16 May 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    NTP + H2O = NDP + phosphate.SAAS annotation
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SAAS annotation
    Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.SAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi1472 – 14721ZincImported
    Metal bindingi1479 – 14791Zinc; via tele nitrogenImported
    Metal bindingi1481 – 14811Zinc; via pros nitrogenImported
    Metal bindingi1490 – 14901ZincImported

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. ion channel activity Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW
    5. RNA binding Source: UniProtKB-KW
    6. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    7. RNA helicase activity Source: InterPro
    8. structural molecule activity Source: InterPro

    GO - Biological processi

    1. pore formation by virus in membrane of host cell Source: UniProtKB-KW
    2. protein oligomerization Source: UniProtKB-KW
    3. RNA-protein covalent cross-linking Source: InterPro
    4. transcription, DNA-templated Source: InterPro
    5. viral entry into host cell Source: UniProtKB-KW
    6. viral RNA genome replication Source: InterPro
    7. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    HelicaseSAAS annotation, Hydrolase, Ion channel, Nucleotidyltransferase, Protease, RNA-directed RNA polymeraseSAAS annotation, Thiol proteaseSAAS annotation, Transferase, Viral ion channelSAAS annotation

    Keywords - Biological processi

    Host-virus interaction, Ion transport, Transport, Viral attachment to host cellSAAS annotation, Viral RNA replicationSAAS annotation, Virus entry into host cell

    Keywords - Ligandi

    ATP-binding, Metal-bindingImported, Nucleotide-binding, RNA-bindingSAAS annotation, ZincImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    PolyproteinImported
    OrganismiHuman poliovirus 1Imported
    Taxonomic identifieri12080 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeEnterovirusEnterovirus C

    Subcellular locationi

    Host cytoplasmic vesicle membrane SAAS annotation; Peripheral membrane protein SAAS annotation; Cytoplasmic side SAAS annotation

    GO - Cellular componenti

    1. host cell cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    2. integral to membrane of host cell Source: UniProtKB-KW
    3. membrane Source: UniProtKB-KW
    4. viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid proteinSAAS annotation, Host cytoplasmSAAS annotation, Host cytoplasmic vesicleSAAS annotation, Host membraneSAAS annotation, Membrane, Virion

    PTM / Processingi

    Keywords - PTMi

    PhosphoproteinSAAS annotation

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2IJDX-ray3.401/21027-1670[»]
    ProteinModelPortaliQ1PHW8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains RdRp catalytic domain.SAAS annotation
    Contains SF3 helicase domain.SAAS annotation
    Contains SFhelicase domain.SAAS annotation

    Family and domain databases

    Gene3Di2.60.120.20. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR014838. P3A.
    IPR000081. Peptidase_C3.
    IPR000199. Peptidase_C3A/C3B_picornavir.
    IPR002527. Pico_P2B.
    IPR001676. Picornavirus_capsid.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view]
    PfamiPF08727. P3A. 1 hit.
    PF00548. Peptidase_C3. 1 hit.
    PF00947. Pico_P2A. 1 hit.
    PF01552. Pico_P2B. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00073. Rhv. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view]
    ProDomiPD001306. Peptidase_C3. 1 hit.
    PD649346. Pico_P2B. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF89043. SSF89043. 1 hit.
    PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Q1PHW8-1 [UniParc]FASTAAdd to Basket

    « Hide

    VVPLSTPREM DILGFVSACN DFSVRLLRDT THIEQKALAQ GLGQMLESMI     50
    DNTVRETVGA ATSRDALPNT EASGPAHSKE IPALTAVETG ATNPLVPSDT 100
    VQTRHVIQHR SRSESSIESF FARGACVTIM TVDNSASTGS KDKLFTVWKI 150
    TYKDTVQLRR KLEFFTYSRF DMEFTFVVTA NFTETNNGHA LNQVYQIMYV 200
    PPGAPVPKEW DDYTWQTSSN PSIFYTYGTA PARISIPYVG ISNAYSHFYD 250
    GFSKIPLKDQ TAALGDSLYG AASLNDFGIL AVRVVNDHNP TKVTSKIRVY 300
    LKPKHIRVWC PRPPRAVAYY GPGVDYKDGT LTPLPTKDLT TYGFGHQNKA 350
    VYTAGYKICN YHLATQEDLQ NAVNVMWDRD LLVTESRSQG TDSIARCNCN 400
    TGVYYCESRR KYYPVSFVGP TFQYMEANDY YPARYQSHML IGHGFASPGD 450
    CGGILRCRHG VIGIITAGGE GLVAFSDIRD LYAYEEEAME QGITNYIESL 500
    GAAFGSGFTQ QIGDKITELT NMVTSTITEK LLKNLIKIIS SLVIITRNYE 550
    DTTTVLATLA LLGCDISPWQ WLKKKACDVL EIPYVIKQGD SWLKKFTEAC 600
    NAAKGLEWVS NKISKFIDWL KEKIIPQARD KLEFVTKLRQ LEMLENQIST 650
    IHQSCPSQEH QEILFNNVRW LSIQSKRFAP LYAVEAKRIQ KLEHTINNYI 700
    QFKSKHRIEP VCLLVHGSPG TGKSVATNLI ARAIAERENT STYSLPPDPS 750
    HFDGYKQQGV VIMDDLNQNP DGADMKLFCQ MVSTVEFIPP MASLEEKGIL 800
    FTSNYVLAST NSSRISPPTV AHSDALARRF AFDMDIQVMN EYSRDGKLNM 850
    AMATEMCKNC HQPANFKRCC PLVCGKAIQL MDKSSRVRYS IDQITTMIIN 900
    ERNRRSNIGN CMEALFQGPL QYKDLKIDIK TSPPPECIND LLQAVDSQEV 950
    RDYCEKKGWI VNITSQVQTE RNINRAMTIL QAVTTFAAVA GVVYVMYKLF 1000
    AGHQGAYTGL PNKKPNVPTI RTAKVQGPGF DYAVAMAKRN IVTATTSKGE 1050
    FTMLGVHDNV AILPTHASPG ESIVIDGKEV EILDAKALED QAGTNLEITI 1100
    ITLKRNEKFR DIRPHIPTQI TETNDGVLIV NTSKYPNMYV PVGAVTEQGY 1150
    LNLGGRQTAR TLMYNFPTRA GQCGGVITCT GKVIGMHVGG NGSHGFAAAL 1200
    KRSYFTQSQG EIQWMRPSKE VGYPIINAPS KTKLEPSAFH YVFEGVKEPA 1250
    VLTKNDPRLK TDFEEAIFSK YVGNKITEVD EYMKEAVDHY AGQLMSLDIN 1300
    TEQMCLEDAM YGTDGLEALD LSTSAGYPYV AMGKKKRDIL NKQTRDTKEM 1350
    QKLLDTYGIN LPLVTYVKDE LRSKTKVEMG KSRLIEASSL NDSVAMRMAF 1400
    GNLYAAFHKN PGVVTGSAVG CDPDLFWSKI PVLMEEKLFA FDYTGYDASL 1450
    SPAWFEALKM VLEKIGFGDR VDYIDYLNHS HHLYKNKTYC VKGGMPSGCS 1500
    GTSIFNSMIN NLIIRTLLLK TYKGIDLDHL KMIAYGDDVI ASYPHEVDAS 1550
    LLAQSGKDYG LTMTPADKSA TFETVTWENV TFLKRFFRAD EKYPFLIHPV 1600
    MPMKEIHESI RWTKDPRNTQ DHVRSLCLLA WHNGEEEYNK FLAKIRSVPI 1650
    GRALLLPEYS TLYRRWLDSF 1670
    Length:1,670
    Mass (Da):186,996
    Last modified:May 16, 2006 - v1
    Checksum:i31D825913C635FCE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11Imported

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ411563 Genomic RNA. Translation: ABE02680.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ411563 Genomic RNA. Translation: ABE02680.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2IJD X-ray 3.40 1/2 1027-1670 [» ]
    ProteinModelPortali Q1PHW8.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.20. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR014838. P3A.
    IPR000081. Peptidase_C3.
    IPR000199. Peptidase_C3A/C3B_picornavir.
    IPR002527. Pico_P2B.
    IPR001676. Picornavirus_capsid.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view ]
    Pfami PF08727. P3A. 1 hit.
    PF00548. Peptidase_C3. 1 hit.
    PF00947. Pico_P2A. 1 hit.
    PF01552. Pico_P2B. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00073. Rhv. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view ]
    ProDomi PD001306. Peptidase_C3. 1 hit.
    PD649346. Pico_P2B. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF89043. SSF89043. 1 hit.
    PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genetic drift of poliovirus type 1 during 51 months of prolonged intestinal replication in an immunodeficient patient."
      Vollnberg B., Diedrich S., Schreier E.
      Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: PV1/00-87/DEUImported.
    2. "Crystal structure of poliovirus 3CD protein: virally encoded protease and precursor to the RNA-dependent RNA polymerase."
      Marcotte L.L., Wass A.B., Gohara D.W., Pathak H.B., Arnold J.J., Filman D.J., Cameron C.E., Hogle J.M.
      J. Virol. 81:3583-3596(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.40 ANGSTROMS) OF 1027-1670 IN COMPLEX WITH ZINC.

    Entry informationi

    Entry nameiQ1PHW8_9ENTO
    AccessioniPrimary (citable) accession number: Q1PHW8
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 16, 2006
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3