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Protein

L-ascorbate peroxidase 2, cytosolic

Gene

APX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Plays a key role in hydrogen peroxide removal.By similarity

Miscellaneous

Binds one cation per subunit; probably K+, but might also be Ca2+.By similarity

Catalytic activityi

2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.

Cofactori

heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei39Transition state stabilizerPROSITE-ProRule annotation1
Active sitei43Proton acceptorPROSITE-ProRule annotation1
Metal bindingi163Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi164Potassium or calciumBy similarity1
Metal bindingi180Potassium or calciumBy similarity1
Metal bindingi182Potassium or calciumBy similarity1
Metal bindingi185Potassium or calcium; via carbonyl oxygenBy similarity1
Metal bindingi187Potassium or calciumBy similarity1

GO - Molecular functioni

  • cytochrome-c peroxidase activity Source: GO_Central
  • heme binding Source: InterPro
  • L-ascorbate peroxidase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processHydrogen peroxide
LigandCalcium, Heme, Iron, Metal-binding, Potassium

Enzyme and pathway databases

BioCyciARA:AT3G09640-MONOMER

Protein family/group databases

PeroxiBasei1888 AtAPx02

Names & Taxonomyi

Protein namesi
Recommended name:
L-ascorbate peroxidase 2, cytosolic (EC:1.11.1.11)
Alternative name(s):
L-ascorbate peroxidase 1b
Short name:
APX1b
Short name:
AtAPx02
Gene namesi
Name:APX2
Synonyms:APX1B
Ordered Locus Names:At3g09640
ORF Names:F11F8_23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G09640
TAIRilocus:2074914 AT3G09640

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002613221 – 251L-ascorbate peroxidase 2, cytosolicAdd BLAST251

Proteomic databases

PaxDbiQ1PER6
PRIDEiQ1PER6

Expressioni

Tissue specificityi

Detected in bundle sheath cells, the photosynthetic cells that surround the phloem and xylem.1 Publication

Inductioni

By excess light treatment, by wounding and by heat-shock stress.4 Publications

Gene expression databases

ExpressionAtlasiQ1PER6 differential
GenevisibleiQ1PER6 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G09640.1

Structurei

3D structure databases

ProteinModelPortaliQ1PER6
SMRiQ1PER6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IF5T Eukaryota
COG0376 LUCA
InParanoidiQ1PER6
KOiK00434
OMAiHDANNGL
OrthoDBiEOG09360KYY
PhylomeDBiQ1PER6

Family and domain databases

InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR002016 Haem_peroxidase_pln/fun/bac
IPR002207 Peroxidase_I
IPR019794 Peroxidases_AS
IPR019793 Peroxidases_heam-ligand_BS
PfamiView protein in Pfam
PF00141 peroxidase, 1 hit
PRINTSiPR00459 ASPEROXIDASE
PR00458 PEROXIDASE
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS00435 PEROXIDASE_1, 1 hit
PS00436 PEROXIDASE_2, 1 hit
PS50873 PEROXIDASE_4, 1 hit

Sequencei

Sequence statusi: Complete.

Q1PER6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKKSYPEVK EEYKKAVQRC KRKLRGLIAE KHCAPIVLRL AWHSAGTFDV
60 70 80 90 100
KTKTGGPFGT IRHPQELAHD ANNGLDIAVR LLDPIKELFP ILSYADFYQL
110 120 130 140 150
AGVVAVEITG GPEIPFHPGR LDKVEPPPEG RLPQATKGVD HLRDVFGRMG
160 170 180 190 200
LNDKDIVALS GGHTLGRCHK ERSGFEGAWT PNPLIFDNSY FKEILSGEKE
210 220 230 240 250
GLLQLPTDKA LLDDPLFLPF VEKYAADEDA FFEDYTEAHL KLSELGFADK

E
Length:251
Mass (Da):28,006
Last modified:January 23, 2007 - v3
Checksum:iFFC7F6D82A4EF3E0
GO

Sequence cautioni

The sequence AAF23294 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6Y → F in ABE65932 (PubMed:17147637).Curated1
Sequence conflicti231F → S in CAA66925 (PubMed:8580771).Curated1
Sequence conflicti231F → S in CAA56340 (PubMed:9144965).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80036 Genomic DNA Translation: CAA56340.1
X98275 mRNA Translation: CAA66925.1
AC016661 Genomic DNA Translation: AAF23294.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE74791.1
CP002686 Genomic DNA Translation: AEE74792.1
AK176821 mRNA Translation: BAD44584.1
AK176908 mRNA Translation: BAD44671.1
DQ446651 mRNA Translation: ABE65932.1
RefSeqiNP_001030664.1, NM_001035587.3
NP_187575.2, NM_111798.4
UniGeneiAt.129

Genome annotation databases

EnsemblPlantsiAT3G09640.1; AT3G09640.1; AT3G09640
AT3G09640.2; AT3G09640.2; AT3G09640
GeneIDi820121
GrameneiAT3G09640.1; AT3G09640.1; AT3G09640
AT3G09640.2; AT3G09640.2; AT3G09640
KEGGiath:AT3G09640

Similar proteinsi

Entry informationi

Entry nameiAPX2_ARATH
AccessioniPrimary (citable) accession number: Q1PER6
Secondary accession number(s): Q39006, Q67XB1, Q9SF39
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 85 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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