Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q1PEF8 (INV2_ARATH)

Last modified November 3, 2009. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Beta-fructofuranosidase, insoluble isoenzyme CWINV2
    EC=3.2.1.26
Alternative name(s):
    Cell wall invertase 2
      Short name=AtcwINV2
    Sucrose hydrolase 2
    Cell wall beta-fructosidase 2
      Short name=AtbetaFRUCT2
Gene names
Name: CWINV2
Synonyms: BFRUCT2
Ordered Locus Names: At3g52600
ORF Names: F22O6.20, F3C22.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Hide | Top

Protein attributes

Sequence length590 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

Hide | Top

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.

Subcellular location

Secreted › extracellular space › apoplast Probable. Secreted › cell wall Probable. Note: Associated to the cell wall Probable.

Tissue specificity

Expressed in flowers, and seeds. Ref.5 Ref.6

Induction

By aeroponic growth condition, darkness, sucrose, glucose and mannitol. Ref.5

Sequence similarities

Belongs to the glycosyl hydrolase 32 family.

Sequence caution

The sequence ABK28599.1 differs from that shown. Reason: Erroneous termination at position 591. Translated as stop.

The sequence CAB43403.1 differs from that shown. Reason: Erroneous gene model prediction.

Hide | Top

Ontologies

Keywords
   Cellular componentApoplast
Cell wall
Secreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionbeta-fructofuranosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Hide | Top

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q1PEF8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 590565Beta-fructofuranosidase, insoluble isoenzyme CWINV2
PRO_0000348348

Regions

Region59 – 624Substrate binding By similarity
Region121 – 1222Substrate binding By similarity
Region185 – 1862Substrate binding By similarity

Sites

Active site621 By similarity
Binding site781Substrate By similarity
Binding site861Substrate By similarity
Binding site2411Substrate By similarity
Binding site2751Substrate By similarity

Amino acid modifications

Glycosylation1181N-linked (GlcNAc...) Potential
Glycosylation1431N-linked (GlcNAc...) Potential
Glycosylation1801N-linked (GlcNAc...) Probable
Glycosylation3351N-linked (GlcNAc...) Probable
Glycosylation5641N-linked (GlcNAc...) Potential
Disulfide bond435 ↔ 483 By similarity

Experimental info

Sequence conflict2561G → R in AAA63802. Ref.1
Sequence conflict2981G → S in BAC42957. Ref.3
Sequence conflict5901K → KG in ABK28599. Ref.4

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 16, 2006. Version 1.
Checksum: 6B2336244D065E06

FASTA59066,823
        10         20         30         40         50         60 
MSAPKFGYVL LLIVLINISN NGVDAFHKVF KKLQSKSTSL ESVSPLHRTA YHFQPPRHWI 

        70         80         90        100        110        120 
NDPNAPMLYK GVYHLFYQYN PKGAVWGNIV WAHSVSKDLI NWEALEPAIY PSKWFDINGT 

       130        140        150        160        170        180 
WSGSATHVPG KGPVILYTGI TENQTQIQNY AIPQDLSDPY LKTWIKPDDN PIVKPDNGEN 

       190        200        210        220        230        240 
GSAFRDPTTA WFNKKDGYWR MLVGSKRKNR GIAYMYKSRD FKKWVKSKRP IHSRKKTGMW 

       250        260        270        280        290        300 
ECPDFFPVSV TDKKNGLDFS YDGPNAKHVL KVSLDLTRYE YYTLGTYDTK KDRYRPDGYT 

       310        320        330        340        350        360 
PDGWDGLRFD YGNYYASKTF FDDKTNRRIL WGWANESDTV QDDTVKGWAG IQLIPRTILL 

       370        380        390        400        410        420 
DSSGKQLVFW PIEEIESLRG KNVQMTNQKM EMGQRFEVQG ITPAQVDVDV TFNVGNLEKA 

       430        440        450        460        470        480 
EKFDESFATK PLELCNLKGS NVNGGVGPFG LITLATSDLE EYTPVFFRVF KDAASNKPKV 

       490        500        510        520        530        540 
LMCSDAKPSS LKKDTGTDAK ERMYKPSFAG FVDVGLLDGK ISLRSLIDHS VVESFGAKGK 

       550        560        570        580        590 
TVITSRVYPT KAVGEKAHLF VFNNGSQPVT VESLNAWNMQ KPLKMNQGAK

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"A second cell wall invertase encoding gene in Arabidopsis thaliana."
Mercier R.W., Gogarten J.
Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed: 11910074] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
Plant Biotechnol. J. 4:317-324(2006) [PubMed: 17147637] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Expression of the Arabidopsis thaliana invertase gene family."
Tymowska-Lalanne Z., Kreis M.
Planta 207:259-265(1998) [PubMed: 9951726] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
[6]"Roles of cell-wall invertases and monosaccharide transporters in the growth and development of Arabidopsis."
Sherson S.M., Alford H.L., Forbes S.M., Wallace G., Smith S.M.
J. Exp. Bot. 54:525-531(2003) [PubMed: 12508063] [Abstract]
Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

U11033 Genomic DNA. Translation: AAA63802.1.
AL050300 Genomic DNA. Translation: CAB43403.1. Sequence problems.
AK118343 mRNA. Translation: BAC42957.1.
DQ446760 mRNA. Translation: ABE66012.1.
DQ653148 mRNA. Translation: ABK28599.1. Sequence problems.
IPIIPI00523446.
PIRT08439.
RefSeqNP_190828.2.
UniGeneAt.35280

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGH32. Glycoside Hydrolase Family 32.

Proteomic databases

PRIDEQ1PEF8.

Genome annotation databases

GeneID824426.
GenomeReviewsGene locus AT3G52600 in contig BA000014_GR.
NMPDRfig|3702.1.peg.16530.

Organism-specific databases

TAIRAt3g52600.

Phylogenomic databases

OMAINWIALE.

Gene expression databases

GenevestigatorQ1PEF8.

Family and domain databases

InterProIPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
[Graphical view]
PfamPF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTSM00640. Glyco_32. 1 hit.
[Graphical view]
PROSITEPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameINV2_ARATH
AccessionPrimary (citable) accession number: Q1PEF8
Secondary accession number(s): A0MF24 expand/collapse secondary AC list , Q38801, Q8GXA3, Q9SVE0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 16, 2006
Last modified: November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Hide | Top

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents