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Protein

Calcium-dependent protein kinase 18

Gene

CPK18

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100ATPPROSITE-ProRule annotation1
Active sitei197Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 85ATPPROSITE-ProRule annotation9
Calcium bindingi387 – 3981PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi424 – 4352PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi466 – 4773PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi496 – 5074PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-111932 CaMK IV-mediated phosphorylation of CREB
R-ATH-442745 Activation of CaMK IV
R-ATH-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-ATH-69473 G2/M DNA damage checkpoint

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 18 (EC:2.7.11.1)
Gene namesi
Name:CPK18
Ordered Locus Names:At4g36070
ORF Names:T19K4.200
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G36070

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00003633402 – 534Calcium-dependent protein kinase 18Add BLAST533

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1
Modified residuei237PhosphoserineBy similarity1
Modified residuei504PhosphoserineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ1PE17
PRIDEiQ1PE17

PTM databases

iPTMnetiQ1PE17

Expressioni

Gene expression databases

ExpressionAtlasiQ1PE17 differential
GenevisibleiQ1PE17 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT4G36070.2

Structurei

3D structure databases

ProteinModelPortaliQ1PE17
SMRiQ1PE17
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini71 – 331Protein kinasePROSITE-ProRule annotationAdd BLAST261
Domaini374 – 410EF-hand 1PROSITE-ProRule annotationAdd BLAST37
Domaini411 – 446EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini447 – 488EF-hand 3PROSITE-ProRule annotationAdd BLAST42
Domaini489 – 518EF-hand 4PROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni337 – 367Autoinhibitory domainBy similarityAdd BLAST31

Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (337-367) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032 Eukaryota
ENOG410XRMJ LUCA
HOGENOMiHOG000233030
InParanoidiQ1PE17
KOiK13412
PhylomeDBiQ1PE17

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF13499 EF-hand_7, 2 hits
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 4 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 4 hits
PS50222 EF_HAND_2, 4 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q1PE17-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLCFSSPKA TRRGTGSRNP NPDSPTQGKA SEKVSNKNKK NTKKIQLRHQ
60 70 80 90 100
GGIPYGKRID FGYAKDFDNR YTIGKLLGHG QFGFTYVATD NNNGNRVAVK
110 120 130 140 150
RIDKAKMTQP IEVEDVKREV KILQALGGHE NVVGFHNAFE DKTYIYIVME
160 170 180 190 200
LCDGGELLDR ILAKKDSRYT EKDAAVVVRQ MLKVAAECHL RGLVHRDMKP
210 220 230 240 250
ENFLFKSTEE GSSLKATDFG LSDFIKPGVK FQDIVGSAYY VAPEVLKRRS
260 270 280 290 300
GPESDVWSIG VITYILLCGR RPFWDKTQDG IFNEVMRKKP DFREVPWPTI
310 320 330 340 350
SNGAKDFVKK LLVKEPRARL TAAQALSHSW VKEGGEASEV PIDISVLNNM
360 370 380 390 400
RQFVKFSRLK QIALRALAKT INEDELDDLR DQFDAIDIDK NGSISLEEMR
410 420 430 440 450
QALAKDVPWK LKDARVAEIL QANDSNTDGL VDFTEFVVAA LHVNQLEEHD
460 470 480 490 500
SEKWQQRSRA AFDKFDIDGD GFITPEELRL QTGLKGSIEP LLEEADVDED
510 520 530
GRISINEFRR LLRSASLKSK NVKSPPGYQL SQKM
Length:534
Mass (Da):60,214
Last modified:May 16, 2006 - v1
Checksum:i78922D64CB49613F
GO

Sequence cautioni

The sequence CAA18501 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB81516 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022373 Genomic DNA Translation: CAA18501.1 Sequence problems.
AL161588 Genomic DNA Translation: CAB81516.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE86610.1
DQ446901 mRNA Translation: ABE66116.1
PIRiT05500
RefSeqiNP_195331.2, NM_119774.3 [Q1PE17-1]
UniGeneiAt.54625

Genome annotation databases

EnsemblPlantsiAT4G36070.1; AT4G36070.1; AT4G36070 [Q1PE17-1]
GeneIDi829763
GrameneiAT4G36070.1; AT4G36070.1; AT4G36070 [Q1PE17-1]
KEGGiath:AT4G36070

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCDPKI_ARATH
AccessioniPrimary (citable) accession number: Q1PE17
Secondary accession number(s): O65644
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: May 16, 2006
Last modified: May 23, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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