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Protein

Calcium-dependent protein kinase 18

Gene

CPK18

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100ATPPROSITE-ProRule annotation1
Active sitei197Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 85ATPPROSITE-ProRule annotation9
Calcium bindingi387 – 3981PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi424 – 4352PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi466 – 4773PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi496 – 5074PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 18 (EC:2.7.11.1)
Gene namesi
Name:CPK18
Ordered Locus Names:At4g36070
ORF Names:T19K4.200
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G36070.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00003633402 – 534Calcium-dependent protein kinase 18Add BLAST533

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiQ1PE17.
PRIDEiQ1PE17.

PTM databases

iPTMnetiQ1PE17.

Expressioni

Gene expression databases

ExpressionAtlasiQ1PE17. baseline and differential.
GenevisibleiQ1PE17. AT.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT4G36070.2.

Structurei

3D structure databases

ProteinModelPortaliQ1PE17.
SMRiQ1PE17.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini71 – 331Protein kinasePROSITE-ProRule annotationAdd BLAST261
Domaini374 – 410EF-hand 1PROSITE-ProRule annotationAdd BLAST37
Domaini411 – 446EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini447 – 488EF-hand 3PROSITE-ProRule annotationAdd BLAST42
Domaini489 – 518EF-hand 4PROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni337 – 367Autoinhibitory domainBy similarityAdd BLAST31

Domaini

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (337-367) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ1PE17.
KOiK13412.
PhylomeDBiQ1PE17.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q1PE17-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLCFSSPKA TRRGTGSRNP NPDSPTQGKA SEKVSNKNKK NTKKIQLRHQ
60 70 80 90 100
GGIPYGKRID FGYAKDFDNR YTIGKLLGHG QFGFTYVATD NNNGNRVAVK
110 120 130 140 150
RIDKAKMTQP IEVEDVKREV KILQALGGHE NVVGFHNAFE DKTYIYIVME
160 170 180 190 200
LCDGGELLDR ILAKKDSRYT EKDAAVVVRQ MLKVAAECHL RGLVHRDMKP
210 220 230 240 250
ENFLFKSTEE GSSLKATDFG LSDFIKPGVK FQDIVGSAYY VAPEVLKRRS
260 270 280 290 300
GPESDVWSIG VITYILLCGR RPFWDKTQDG IFNEVMRKKP DFREVPWPTI
310 320 330 340 350
SNGAKDFVKK LLVKEPRARL TAAQALSHSW VKEGGEASEV PIDISVLNNM
360 370 380 390 400
RQFVKFSRLK QIALRALAKT INEDELDDLR DQFDAIDIDK NGSISLEEMR
410 420 430 440 450
QALAKDVPWK LKDARVAEIL QANDSNTDGL VDFTEFVVAA LHVNQLEEHD
460 470 480 490 500
SEKWQQRSRA AFDKFDIDGD GFITPEELRL QTGLKGSIEP LLEEADVDED
510 520 530
GRISINEFRR LLRSASLKSK NVKSPPGYQL SQKM
Length:534
Mass (Da):60,214
Last modified:May 16, 2006 - v1
Checksum:i78922D64CB49613F
GO

Sequence cautioni

The sequence CAA18501 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB81516 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022373 Genomic DNA. Translation: CAA18501.1. Sequence problems.
AL161588 Genomic DNA. Translation: CAB81516.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86610.1.
DQ446901 mRNA. Translation: ABE66116.1.
PIRiT05500.
RefSeqiNP_195331.2. NM_119774.3. [Q1PE17-1]
UniGeneiAt.54625.

Genome annotation databases

EnsemblPlantsiAT4G36070.1; AT4G36070.1; AT4G36070. [Q1PE17-1]
GeneIDi829763.
GrameneiAT4G36070.1; AT4G36070.1; AT4G36070.
KEGGiath:AT4G36070.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022373 Genomic DNA. Translation: CAA18501.1. Sequence problems.
AL161588 Genomic DNA. Translation: CAB81516.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86610.1.
DQ446901 mRNA. Translation: ABE66116.1.
PIRiT05500.
RefSeqiNP_195331.2. NM_119774.3. [Q1PE17-1]
UniGeneiAt.54625.

3D structure databases

ProteinModelPortaliQ1PE17.
SMRiQ1PE17.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G36070.2.

PTM databases

iPTMnetiQ1PE17.

Proteomic databases

PaxDbiQ1PE17.
PRIDEiQ1PE17.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G36070.1; AT4G36070.1; AT4G36070. [Q1PE17-1]
GeneIDi829763.
GrameneiAT4G36070.1; AT4G36070.1; AT4G36070.
KEGGiath:AT4G36070.

Organism-specific databases

TAIRiAT4G36070.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ1PE17.
KOiK13412.
PhylomeDBiQ1PE17.

Miscellaneous databases

PROiQ1PE17.

Gene expression databases

ExpressionAtlasiQ1PE17. baseline and differential.
GenevisibleiQ1PE17. AT.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDPKI_ARATH
AccessioniPrimary (citable) accession number: Q1PE17
Secondary accession number(s): O65644
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: May 16, 2006
Last modified: November 30, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.