Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Laccase-16

Gene

LAC16

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Lignin degradation and detoxification of lignin-derived products.By similarity

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi84 – 841Copper 1; type 2By similarity
Metal bindingi86 – 861Copper 2; type 3By similarity
Metal bindingi129 – 1291Copper 2; type 3By similarity
Metal bindingi131 – 1311Copper 3; type 3By similarity
Metal bindingi467 – 4671Copper 4; type 1By similarity
Metal bindingi470 – 4701Copper 1; type 2By similarity
Metal bindingi472 – 4721Copper 3; type 3By similarity
Metal bindingi529 – 5291Copper 3; type 3By similarity
Metal bindingi530 – 5301Copper 4; type 1By similarity
Metal bindingi531 – 5311Copper 2; type 3By similarity
Metal bindingi535 – 5351Copper 4; type 1By similarity

GO - Molecular functioni

  1. copper ion binding Source: InterPro
  2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC

GO - Biological processi

  1. lignin catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G58910-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-16 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase 16
Diphenol oxidase 16
Urishiol oxidase 16
Gene namesi
Name:LAC16
Ordered Locus Names:At5g58910
ORF Names:K19M22.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G58910.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 566539Laccase-16PRO_0000283644Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi389 – 3891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi406 – 4061N-linked (GlcNAc...)Sequence Analysis
Glycosylationi449 – 4491N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Mostly expressed in roots at low level.2 Publications

Gene expression databases

GenevestigatoriQ1PDH6.

Structurei

3D structure databases

ProteinModelPortaliQ1PDH6.
SMRiQ1PDH6. Positions 19-545.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 150114Plastocyanin-like 1Add
BLAST
Domaini160 – 312153Plastocyanin-like 2Add
BLAST
Domaini414 – 550137Plastocyanin-like 3Add
BLAST

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated
Contains 3 plastocyanin-like domains.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG2132.
HOGENOMiHOG000241916.
InParanoidiQ1PDH6.
PhylomeDBiQ1PDH6.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR017761. Laccase.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 4 hits.
TIGRFAMsiTIGR03389. laccase. 1 hit.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q1PDH6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEIPRRFCIC VLTFFVFVLL SPTTVHSIIR HYKFNVMTNT TKLCSSKPIV
60 70 80 90 100
TVNGQFPGPT IVAREGDTIL IKVVNHVKYN VSIHWHGIRQ LRTGWADGPA
110 120 130 140 150
YITQCPIQPG QNYLHNFTLT GQRGTLWWHA HILWLRATVH GAIVILPKLG
160 170 180 190 200
VPYPFPKPYK EKTIVLSEWW KSDVEELINE ASRIGTAPSA SDAHTINGHS
210 220 230 240 250
GSISNCPSQS SYGLPVRAGK TYMLRIINAA LNEELFFKIA GHVLTVVEVD
260 270 280 290 300
AVYTKPYKTD TVFIAPGQTT NVLLTANANA GSNYMVAATT FTDAHIPYDN
310 320 330 340 350
VTATATLHYI GHTSTVSTSK KTVLASLPPQ NATWVATKFT RSLRSLNSLE
360 370 380 390 400
YPARVPTTVE HSLFFTVGLG ANPCQSCNNG VRLVAGINNV TFTMPKTALL
410 420 430 440 450
QAHFFNISGV FTDDFPAKPS NPYDYTAPVK LGVNAATMKG TKLYRLPYNA
460 470 480 490 500
TVQIVLQNTA MILSDNHPFH LHGFNFFEVG RGLGNFNPEK DPKAFNLVDP
510 520 530 540 550
VERNTVGVPA GGWTAIRFIA DNPGVWFMHC HLELHTTWGL KMAFVVDNGH
560
GPDQSLLPPP ADLPKC
Length:566
Mass (Da):62,373
Last modified:April 3, 2007 - v2
Checksum:i4BCFEEC24628B5FB
GO

Sequence cautioni

The sequence ABK28766.1 differs from that shown. Reason: Erroneous termination at position 567. Translated as stop.Curated
The sequence AED97116.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016885 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED97116.1. Sequence problems.
DQ447092 mRNA. Translation: ABE66261.1.
DQ653381 mRNA. Translation: ABK28766.1. Sequence problems.
RefSeqiNP_200699.1. NM_125281.1.
UniGeneiAt.55619.

Genome annotation databases

EnsemblPlantsiAT5G58910.1; AT5G58910.1; AT5G58910.
GeneIDi836008.
KEGGiath:AT5G58910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016885 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED97116.1. Sequence problems.
DQ447092 mRNA. Translation: ABE66261.1.
DQ653381 mRNA. Translation: ABK28766.1. Sequence problems.
RefSeqiNP_200699.1. NM_125281.1.
UniGeneiAt.55619.

3D structure databases

ProteinModelPortaliQ1PDH6.
SMRiQ1PDH6. Positions 19-545.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G58910.1; AT5G58910.1; AT5G58910.
GeneIDi836008.
KEGGiath:AT5G58910.

Organism-specific databases

TAIRiAT5G58910.

Phylogenomic databases

eggNOGiCOG2132.
HOGENOMiHOG000241916.
InParanoidiQ1PDH6.
PhylomeDBiQ1PDH6.

Enzyme and pathway databases

BioCyciARA:AT5G58910-MONOMER.

Gene expression databases

GenevestigatoriQ1PDH6.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR017761. Laccase.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 4 hits.
TIGRFAMsiTIGR03389. laccase. 1 hit.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
    Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
    Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
    Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 37-566.
    Strain: cv. Columbia.
  4. "Gene structure and molecular analysis of the laccase-like multicopper oxidase (LMCO) gene family in Arabidopsis thaliana."
    McCaig B.C., Meagher R.B., Dean J.F.D.
    Planta 221:619-636(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Mutant identification and characterization of the laccase gene family in Arabidopsis."
    Cai X., Davis E.J., Ballif J., Liang M., Bushman E., Haroldsen V., Torabinejad J., Wu Y.
    J. Exp. Bot. 57:2563-2569(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiLAC16_ARATH
AccessioniPrimary (citable) accession number: Q1PDH6
Secondary accession number(s): A0MFQ5, F4KGE3, Q3E893
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: January 7, 2015
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.