ID   L_MABVR                 Reviewed;        2327 AA.
AC   Q1PDC4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=Large structural protein;
DE            Short=Protein L;
DE   AltName: Full=Transcriptase;
DE   AltName: Full=Replicase;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=mRNA (guanine-N(7)-)-methyltransferase;
DE              EC=2.1.1.56;
DE   Includes:
DE     RecName: Full=mRNA guanylyltransferase;
DE              EC=2.7.7.-;
GN   Name=L;
OS   Lake Victoria marburgvirus (strain Ravn-87) (MARV) (Marburg virus
OS   (strain Kenya/Ravn/1987)).
OC   Viruses; ssRNA negative-strand viruses; Mononegavirales; Filoviridae;
OC   Marburgvirus.
OX   NCBI_TaxID=378809;
OH   NCBI_TaxID=9407; Rousettus aegyptiacus (Egyptian rousette) (Egyptian fruit bat).
OH   NCBI_TaxID=9534; Cercopithecus aethiops (Green monkey) (Grivet).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=16775337; DOI=10.1128/JVI.00069-06;
RA   Towner J.S., Khristova M.L., Sealy T.K., Vincent M.J., Erickson B.R.,
RA   Bawiec D.A., Hartman A.L., Comer J.A., Zaki S.R., Stroeher U.,
RA   Gomes da Silva F., del Castillo F., Rollin P.E., Ksiazek T.G.,
RA   Nichol S.T.;
RT   "Marburgvirus genomics and association with a large hemorrhagic fever
RT   outbreak in Angola.";
RL   J. Virol. 80:6497-6516(2006).
CC   -!- FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl
CC       transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A)
CC       synthetase activities. The viral mRNA guanylyl transferase
CC       displays a different biochemical reaction than the cellular
CC       enzyme. The template is composed of the viral RNA tightly
CC       encapsidated by the nucleoprotein (N). Functions either as
CC       transcriptase or as replicase. The transcriptase synthesizes
CC       subsequently subgenomic RNAs, assuring their capping and
CC       polyadenylation by a stuttering mechanism. The replicase mode is
CC       dependent on intracellular N protein concentration. In this mode,
CC       the polymerase replicates the whole viral genome without
CC       recognizing the transcriptional signals (By similarity).
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-
CC       adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm. Virion (By similarity).
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; DQ447649; ABE27074.1; -; Genomic_RNA.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019012; C:virion; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:EC.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006370; P:mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   InterPro; IPR017235; RNA-dir_RNA_pol_filovir.
DR   InterPro; IPR014023; RNA_pol_cat.
DR   InterPro; IPR001016; RNA_pol_L_viral.
DR   Pfam; PF00946; Paramyx_RNA_pol; 1.
DR   PIRSF; PIRSF037548; RNA_pol_Filoviridae; 1.
DR   PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; Methyltransferase;
KW   mRNA capping; mRNA processing; Multifunctional enzyme;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA replication;
KW   RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase;
KW   Virion.
FT   CHAIN         1   2327       Large structural protein.
FT                                /FTId=PRO_0000314972.
FT   DOMAIN      628    812       RdRp catalytic.
SQ   SEQUENCE   2327 AA;  266524 MW;  3F4516E0D40608F1 CRC64;
     MQHPTQYPDA RLSSPIILDQ CDLLTRSLGL YSHYSHNPKL RNCRIPYHIY RLRNSTALKT
     FLQNCSILTV PFHSIWDHII TSIQHDAINH INDFKYLLPS ELIKYANWDN EFLRVFLNKI
     LRLDHAFTNS AKLQCEDFSP KENPYYWGML LLVHLSQLAR RIKGQRGSLR SNWKFIGVDL
     ELFGIADFVI FKVPIKAIIR NATSLQASKP GLKTWYRDQN LTPYLCDDEF VVSIASYECF
     IMIKDVFIER YNTWEICARA WVEDNEEADY PPLGILRDLY NQGDQIITMY LEDGFKLIKH
     LEPLCVSCIQ TYGIFTPRKY WFQSQMIKSY YDELQSLNLK LQIPDNRTEC AQNFIKTIIQ
     AKLTPQQYCE LFSLQKHWGH PVLYNDVALD KVKKHAQSTK ILKPKVMFET FCVFKFIVAK
     NHYHSQGSWY KTTHDLHLTP YLRQHIVSNS FPSQAEIYQH LWEWYFVEHE PLFSTKIISD
     LSIFIKDRAT AVNRECWDSV FDRSVLGYNP PVRFQSKRVP EQFLGQADFS LNQILDFAEK
     LEYLAPSYRN FSFSLKEKEL NIGRTFGKLP YRVRNVQTLA EALLADGLAK AFPSNMMVVT
     EREQKEALLH QASWHHNSAS IGENAIVRGA SFVTDLEKYN LAFRYEFTRH FIDYCNRCYG
     VKNLFDWMHF LIPLCYMHVS DFYSPPHCVT ENNRNNPPDC ANAYHYHLGG IEGLQQKLWT
     CISCAQITLV ELKTKLKLKS SVMGDNQCIT TLSLFPIDAP NDYQENEAEL NAARVAVELA
     ITTGYSGIFL KPEETFVHSG FIYFGKKQYL NGVQLPQSLK TMARCGPLSD SIFDDLQGSL
     ASIGTSFERG ASETRHIFPS RWIAAFHSML AVNLLNQNHL GFPLGFSIDV SCFKKPLTFS
     EKLIALITPQ VLGGLSFLNP EKLFYRNISD PLTSGLFQLR NALEFLRKEE LFYILIAKKP
     GLADASDFVM NPLGLNVPGS REIITFLRQT VRENITITSQ NRIINSLFHI GSDLEDQRVC
     EWLLSSNPVM SRFAADIFSR TPSGKRLQVL GYLEGTRTLL ASRTISLTTE GTMLMRLREL
     TKSRWKSWFS YIDALDDDLS ESLEKFICTV DVANFLRAYS WSDVLKGKRL IGATLPCLLE
     QFNVKWVNLS EDLKEQFKLS SDLGSPTDLL QYDCNGLHSK GADNAELNYV SCALDRKIVQ
     KHPSDNRLAW TIGNRAPYIG SRTEDKIGYP PLRVNCPSAA LKEAIEMVSR LLWVTQGTAD
     REKLLIPLLN SRVNLDYQTV LNFLPTHYSG NIVHRYNDQY GQHSFMANRM SNTSTRAIIS
     TNTLGKYAGG GQAAVDSNII FQNTINLGVA VLDITLSLSK LSSTSNVSFR LMLSKCCTRH
     VPSEYLFFDK PLDVDLNKYM DNELVYDNDP LCSGIKGRLG RVSRSTLSLS LNVSDIGSYD
     FPTIAAWTLG ETIIGSIFSD ESSQSTDPIS SGCTKTFVTH FLVYPVESIF YAFGANLIVE
     SLSLSRINSI KSLSDLTFLI SSTIRNLSHR SLRILQSTFR HELVLTRLAH HIPLISLMLG
     GSAGEKSSSD AVRLFLTASY QNFINNFSCL MRKNQSPLPV WLYFPSEGQQ LKPILKILQR
     LSCLLTTKKV QNHRPVADTC FLTDNFWVYP SKSTRTNHYY ASLNYWRDKA NKIKNTSFSH
     LINYSFSEPS LHASSISSSQ EVVNLKHTSR LDETPNMSER AQSTNHEPTA LQEVCTEIPY
     SEQDPAKSYL LLENTRFRDD QKILRHDQKA ERGEPLSLQV SSRGCLQALT CPHHPSPSQT
     TTEPLSMLRN CDAIKAALRS ETNDPRLMSS ILDMRSLKTP MRIESRNTSL LQPSECLSTS
     KGKSVLSREQ ASYLYVDCSN ISSISLDSGF RNMSDRNQVQ MLINTYKRDL YTCFDSNQFC
     RFTGVVSSMH YKLYDLLPAG KLGKAICLAE GEGSGARLLL KWKETDYLFF NTLATDSQQE
     AEILSGRVIP RMLYNIDKLS VLLESRKLIL NNLTIQITDI TNPLWLDSVI QYLPEDSDIL
     TMDAETTKEE TREQLYKTII NIWARTSPNI PKTSIIKVFL LDYGGTLFLM KNAIQYYGQV
     QLKKPYSSNA KNSEWYLCCG KRRVQRLRVD FPDQVGIFLI CKAMSRQRQA IPYWLKHIEK
     NYPASLHEFF ITLGFPSLES SFCHRYTIPF TEGTALFHKV QSYVRQGRQH LHSLMLDYEN
     NSPLLDLRNH FICSLRGKIT KYYNDILKLN LVVRAVERGK NWSQLVESLP NMHSVCITHV
     DHECIGCERR LLLKLDFVRN TKIAEQKLLN RVIGYILFFP FGFSRPK
//