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Protein

Protein inscuteable homolog

Gene

INSC

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex (PubMed:16458856). Involved in spindle orientation during mitosis. May regulate cell proliferation and differentiation in the developing nervous system. May play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium (By similarity).By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Protein inscuteable homolog
Gene namesi
Name:INSC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:33116. INSC.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cytoplasmcell cortex 1 Publication

  • Note: Uniformly distributed in the cytoplasm during interphase. During metaphase, detected in the cell cortex, adjacent to the mitotic spindle poles.1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi78 – 781W → A: Abolishes interaction with GPSM2. 1 Publication
Mutagenesisi89 – 891E → R: Strongly reduces interaction with GPSM2. Abolishes interaction with GPSM2 and GPSM1; when associated with D-97. 1 Publication
Mutagenesisi97 – 971K → D: Abolishes interaction with GPSM2 and GPSM1; when associated with R-89. 1 Publication

Organism-specific databases

PharmGKBiPA162392191.

Polymorphism and mutation databases

DMDMi116248172.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 579579Protein inscuteable homologPRO_0000252405Add
BLAST

Proteomic databases

PaxDbiQ1MX18.
PRIDEiQ1MX18.

PTM databases

iPTMnetiQ1MX18.
PhosphoSiteiQ1MX18.

Expressioni

Tissue specificityi

Isoform 1 is expressed in various tissues with stronger expression in liver, kidney and small intestine. Isoform 2 is abundantly expressed in small intestine and to a lower extent in lung and pancreas.2 Publications

Developmental stagei

Expressed in fetal cochlea.1 Publication

Gene expression databases

BgeeiQ1MX18.
CleanExiHS_INSC.
ExpressionAtlasiQ1MX18. baseline and differential.
GenevisibleiQ1MX18. HS.

Organism-specific databases

HPAiHPA039769.

Interactioni

Subunit structurei

Interacts with ALS2CR19/PAR3-beta and F2RL2/PAR3 (PubMed:16458856). Interacts with GPSM1/AGS3 and GPSM2/LGN (via TPR repeat region) (PubMed:16458856, PubMed:22074847). Identified in a complex with GPSM2 and F2RL2 (PubMed:16458856).2 Publications

Protein-protein interaction databases

BioGridi132426. 1 interaction.
STRINGi9606.ENSP00000368872.

Structurei

Secondary structure

1
579
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi73 – 8210Combined sources
Beta strandi91 – 955Combined sources
Helixi103 – 1053Combined sources
Beta strandi109 – 1124Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SF4X-ray2.60D/E/F70-116[»]
ProteinModelPortaliQ1MX18.
SMRiQ1MX18. Positions 70-115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni74 – 8916Important for interaction with GPSM21 PublicationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi576 – 5794PDZ-binding

Phylogenomic databases

eggNOGiENOG410IHN9. Eukaryota.
ENOG410XSNH. LUCA.
GeneTreeiENSGT00390000001511.
HOGENOMiHOG000060207.
HOVERGENiHBG079606.
InParanoidiQ1MX18.
OMAiRANCHVR.
OrthoDBiEOG7SN8D4.
PhylomeDBiQ1MX18.
TreeFamiTF323559.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR031938. INSC_LBD.
[Graphical view]
PfamiPF16748. INSC_LBD. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q1MX18-1) [UniParc]FASTAAdd to basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRPPGNGEA ASEGPGGWGL WGVQESRRLC CAGHDRCKQA LLQIGINMMA
60 70 80 90 100
LPGGRHLDSV TLPGQRLHLM QVDSVQRWME DLKLMTECEC MCVLQAKPIS
110 120 130 140 150
LEEDAQGDLI LAGGPGPGDP LQLLLKRGWV ISTELRRIGQ KLAQDRWARV
160 170 180 190 200
HSMSVRLTCH ARSMVSEYSA VSRNSLKEMG EIEKLLMEKC SELSAVTERC
210 220 230 240 250
LQVENEHVLK SMKACVSETL SMLGQHFGQL LELALTREVQ ALVRKIDASD
260 270 280 290 300
NIYTTESTTG NLFSLTQEGA PLCRIIAKEG GVVALFKVCR QDSFRCLYPQ
310 320 330 340 350
ALRTLASICC VEEGVHQLEK VDGVLCLADI LTDNSHSEAT RAEAAAVVAQ
360 370 380 390 400
VTSPHLPVTQ HLSSFLESME EIVTALVKLC QEASSGEVFL LASAALANIT
410 420 430 440 450
FFDTMACEML LQLNAIRVLL EACSDKQRVD TPYTRDQIVT ILANMSVLEQ
460 470 480 490 500
CASDIIQENG VQLIMGMLSE KPRSGTPAEV AACERVQQKA AVTLARLSRD
510 520 530 540 550
PDVAREAVRL SCMSRLIELC RSPSERNSSD AVLVACLAAL RRLAGVCPEG
560 570
LQDSDFQQLV QPRLVDSFLL CSNMEESFV
Length:579
Mass (Da):63,469
Last modified:May 30, 2006 - v1
Checksum:i7CD8D19409FC3266
GO
Isoform 2 (identifier: Q1MX18-2) [UniParc]FASTAAdd to basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.

Show »
Length:532
Mass (Da):58,454
Checksum:i46C413794E504E42
GO
Isoform 3 (identifier: Q1MX18-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-203: Missing.
     204-238: ENEHVLKSMKACVSETLSMLGQHFGQLLELALTRE → MRREWEARMETHSPIWQNLLMRLDNCLALRLQSPL
     278-319: Missing.

Note: No experimental confirmation available.
Show »
Length:334
Mass (Da):36,669
Checksum:i1B97112A17E1ADE4
GO
Isoform 4 (identifier: Q1MX18-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.
     278-319: Missing.

Note: No experimental confirmation available.
Show »
Length:490
Mass (Da):53,774
Checksum:i00FB28A1F62B99FA
GO
Isoform 5 (identifier: Q1MX18-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.
     239-239: V → VQVESHSVTRLECSGAISAQCNLRLPGSSDSPASAS

Note: No experimental confirmation available.
Show »
Length:567
Mass (Da):61,981
Checksum:i33D225D70DCD9A67
GO
Isoform 6 (identifier: Q1MX18-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.
     539-579: ALRRLAGVCPEGLQDSDFQQLVQPRLVDSFLLCSNMEESFV → MGIQLQVKKTNISALLLWFLFWHIQGCSA

Note: No experimental confirmation available.
Show »
Length:520
Mass (Da):57,188
Checksum:i73DB667C8BEE3113
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti212 – 2121M → T in BAE17134 (Ref. 2) Curated
Sequence conflicti216 – 2161V → M in BAE17135 (Ref. 2) Curated
Sequence conflicti226 – 2261H → R in BAE17133 (Ref. 2) Curated
Sequence conflicti251 – 2511N → D in BAE17134 (Ref. 2) Curated
Sequence conflicti513 – 5131M → T in BAE17135 (Ref. 2) Curated
Sequence conflicti577 – 5782SF → GS in BAE17136 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti333 – 3331D → N.
Corresponds to variant rs17507577 [ dbSNP | Ensembl ].
VAR_051073
Natural varianti450 – 4501Q → R.
Corresponds to variant rs7123855 [ dbSNP | Ensembl ].
VAR_027852

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 203203Missing in isoform 3. 1 PublicationVSP_020946Add
BLAST
Alternative sequencei1 – 4747Missing in isoform 2, isoform 4, isoform 5 and isoform 6. 3 PublicationsVSP_020947Add
BLAST
Alternative sequencei204 – 23835ENEHV…ALTRE → MRREWEARMETHSPIWQNLL MRLDNCLALRLQSPL in isoform 3. 1 PublicationVSP_020948Add
BLAST
Alternative sequencei239 – 2391V → VQVESHSVTRLECSGAISAQ CNLRLPGSSDSPASAS in isoform 5. 1 PublicationVSP_020949
Alternative sequencei278 – 31942Missing in isoform 3 and isoform 4. 1 PublicationVSP_020950Add
BLAST
Alternative sequencei539 – 57941ALRRL…EESFV → MGIQLQVKKTNISALLLWFL FWHIQGCSA in isoform 6. 1 PublicationVSP_054159Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB236158 mRNA. Translation: BAE93463.1.
AB236159 mRNA. Translation: BAE93464.1.
AB231744 mRNA. Translation: BAE46891.1.
AB231745 mRNA. Translation: BAE17133.1.
AB231746 mRNA. Translation: BAE17134.1.
AB231747 mRNA. Translation: BAE17135.1.
AB231748 mRNA. Translation: BAE17136.1.
AC090744 Genomic DNA. No translation available.
BC127700 mRNA. Translation: AAI27701.1.
CCDSiCCDS41621.1. [Q1MX18-1]
CCDS41622.1. [Q1MX18-2]
CCDS60735.1. [Q1MX18-6]
CCDS60736.1. [Q1MX18-4]
RefSeqiNP_001027024.3. NM_001031853.4. [Q1MX18-1]
NP_001036001.1. NM_001042536.2. [Q1MX18-2]
NP_001265242.1. NM_001278313.1. [Q1MX18-2]
NP_001265243.1. NM_001278314.1.
NP_001265244.1. NM_001278315.1. [Q1MX18-6]
NP_001265245.1. NM_001278316.1. [Q1MX18-4]
XP_006718290.1. XM_006718227.2. [Q1MX18-2]
XP_011518384.1. XM_011520082.1. [Q1MX18-2]
XP_011518385.1. XM_011520083.1. [Q1MX18-2]
XP_011518386.1. XM_011520084.1. [Q1MX18-2]
XP_011518388.1. XM_011520086.1. [Q1MX18-6]
UniGeneiHs.591997.

Genome annotation databases

EnsembliENST00000379554; ENSP00000368872; ENSG00000188487. [Q1MX18-1]
ENST00000379556; ENSP00000368874; ENSG00000188487. [Q1MX18-2]
ENST00000525218; ENSP00000436113; ENSG00000188487. [Q1MX18-4]
ENST00000528567; ENSP00000435022; ENSG00000188487. [Q1MX18-6]
ENST00000530161; ENSP00000436194; ENSG00000188487. [Q1MX18-2]
GeneIDi387755.
KEGGihsa:387755.
UCSCiuc001mly.5. human. [Q1MX18-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB236158 mRNA. Translation: BAE93463.1.
AB236159 mRNA. Translation: BAE93464.1.
AB231744 mRNA. Translation: BAE46891.1.
AB231745 mRNA. Translation: BAE17133.1.
AB231746 mRNA. Translation: BAE17134.1.
AB231747 mRNA. Translation: BAE17135.1.
AB231748 mRNA. Translation: BAE17136.1.
AC090744 Genomic DNA. No translation available.
BC127700 mRNA. Translation: AAI27701.1.
CCDSiCCDS41621.1. [Q1MX18-1]
CCDS41622.1. [Q1MX18-2]
CCDS60735.1. [Q1MX18-6]
CCDS60736.1. [Q1MX18-4]
RefSeqiNP_001027024.3. NM_001031853.4. [Q1MX18-1]
NP_001036001.1. NM_001042536.2. [Q1MX18-2]
NP_001265242.1. NM_001278313.1. [Q1MX18-2]
NP_001265243.1. NM_001278314.1.
NP_001265244.1. NM_001278315.1. [Q1MX18-6]
NP_001265245.1. NM_001278316.1. [Q1MX18-4]
XP_006718290.1. XM_006718227.2. [Q1MX18-2]
XP_011518384.1. XM_011520082.1. [Q1MX18-2]
XP_011518385.1. XM_011520083.1. [Q1MX18-2]
XP_011518386.1. XM_011520084.1. [Q1MX18-2]
XP_011518388.1. XM_011520086.1. [Q1MX18-6]
UniGeneiHs.591997.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SF4X-ray2.60D/E/F70-116[»]
ProteinModelPortaliQ1MX18.
SMRiQ1MX18. Positions 70-115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi132426. 1 interaction.
STRINGi9606.ENSP00000368872.

PTM databases

iPTMnetiQ1MX18.
PhosphoSiteiQ1MX18.

Polymorphism and mutation databases

DMDMi116248172.

Proteomic databases

PaxDbiQ1MX18.
PRIDEiQ1MX18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379554; ENSP00000368872; ENSG00000188487. [Q1MX18-1]
ENST00000379556; ENSP00000368874; ENSG00000188487. [Q1MX18-2]
ENST00000525218; ENSP00000436113; ENSG00000188487. [Q1MX18-4]
ENST00000528567; ENSP00000435022; ENSG00000188487. [Q1MX18-6]
ENST00000530161; ENSP00000436194; ENSG00000188487. [Q1MX18-2]
GeneIDi387755.
KEGGihsa:387755.
UCSCiuc001mly.5. human. [Q1MX18-1]

Organism-specific databases

CTDi387755.
GeneCardsiINSC.
HGNCiHGNC:33116. INSC.
HPAiHPA039769.
MIMi610668. gene.
neXtProtiNX_Q1MX18.
PharmGKBiPA162392191.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHN9. Eukaryota.
ENOG410XSNH. LUCA.
GeneTreeiENSGT00390000001511.
HOGENOMiHOG000060207.
HOVERGENiHBG079606.
InParanoidiQ1MX18.
OMAiRANCHVR.
OrthoDBiEOG7SN8D4.
PhylomeDBiQ1MX18.
TreeFamiTF323559.

Miscellaneous databases

GenomeRNAii387755.
NextBioi101596.
PROiQ1MX18.
SOURCEiSearch...

Gene expression databases

BgeeiQ1MX18.
CleanExiHS_INSC.
ExpressionAtlasiQ1MX18. baseline and differential.
GenevisibleiQ1MX18. HS.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR031938. INSC_LBD.
[Graphical view]
PfamiPF16748. INSC_LBD. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two forms of human Inscuteable-related protein that links Par3 to the Pins homologues LGN and AGS3."
    Izaki T., Kamakura S., Kohjima M., Sumimoto H.
    Biochem. Biophys. Res. Commun. 341:1001-1006(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH ALS2CR19; F2RL2; GPSM1 AND GPSM2.
    Tissue: Kidney and Small intestine.
  2. "Identification of novel human genes predicted by combining multiple gene finders."
    Totoki Y., Yada T., Sakaki Y., Takeda T.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3; 4 AND 5).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 6).
  5. "Identification and characterization of human Inscuteable gene in silico."
    Katoh M., Katoh M.
    Int. J. Mol. Med. 11:111-116(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  6. "Structural basis for interaction between the conserved cell polarity proteins Inscuteable and Leu-Gly-Asn repeat-enriched protein (LGN)."
    Yuzawa S., Kamakura S., Iwakiri Y., Hayase J., Sumimoto H.
    Proc. Natl. Acad. Sci. U.S.A. 108:19210-19215(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 70-116 IN COMPLEX WITH GPSM2, INTERACTION WITH GPSM2 AND GPSM1, SUBCELLULAR LOCATION, MUTAGENESIS OF TRP-78; GLU-89 AND LYS-97.

Entry informationi

Entry nameiINSC_HUMAN
AccessioniPrimary (citable) accession number: Q1MX18
Secondary accession number(s): A0PJX5
, Q1MX19, Q3C1V6, Q4AC95, Q4AC96, Q4AC97, Q4AC98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: May 30, 2006
Last modified: May 11, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.