Reviewed, UniProtKB/Swiss-Prot Q1MTI4 (TPISA_DANRE)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase A
      Short name=TIM-A
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase A

Gene names

Name:	tpi1a
ORF Names:	si:dkey-89b17.2

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate.
Pathway	Carbohydrate biosynthesis; gluconeogenesis. Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Gluconeogenesis Glycolysis Lipid synthesis Pentose shunt
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 248	247	Triosephosphate isomerase A		PRO_0000345140
Sites
	Active site	95	1	Electrophile By similarity
	Active site	165	1	Proton acceptor By similarity
	Binding site	11	1	Substrate By similarity
	Binding site	13	1	Substrate By similarity
Experimental info
	Sequence conflict	36	1	E → K in AAK85203. Ref.1
	Sequence conflict	134	1	R → K in AAH49500. Ref.2
	Sequence conflict	179	1	E → G in AAH49500. Ref.2
	Sequence conflict	190	1	G → E in AAK85203. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q1MTI4-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 546B461C6CBD1610	FASTA	248	26,853
	10 20 30 40 50 60 MSSRKFFVGG NWKMNGDKES LGELIMTLNT ASLNDETDVV CGAPSIYLDY ARSKLDQRIG 70 80 90 100 110 120 VAAQNCYKVP KGAFTGEISP AMIKDCGIDW VILGHSERRH VFGESDELIG QKVAHCLESD 130 140 150 160 170 180 LGVIACIGEK LEEREAGTTE DVVFEQTKVI ADNVKDWTRV VLAYEPVWAI GTGKTASPEQ 190 200 210 220 230 240 AQEVHEKLRG WLRANVSDAV ADSVRIIYGG SVTGGNCKEL AAQADVDGFL VGGASLKPEF VDIINARS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for a period of directional selection following gene duplication in a neurally expressed locus of triosephosphate isomerase." Merritt T.J.S., Quattro J.M. Genetics 159:689-697(2001) [PubMed: 11606544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	The Danio rerio sequencing project at the Sanger Institute Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF387820 mRNA. Translation: AAK85203.1. AL772314 Genomic DNA. Translation: CAK11229.1. BC049500 mRNA. Translation: AAH49500.1.
IPI	IPI00498329.
RefSeq	NP_705953.1.
UniGene	Dr.82679
3D structure databases
SMR	Q1MTI4. Positions 2-247.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1MTI4.
Genome annotation databases
Ensembl	ENSDART00000037007; ENSDARP00000033907; ENSDARG00000025012; Danio rerio. [Genome view]
GeneID	192309.
KEGG	dre:192309.
NMPDR	fig|7955.3.peg.9678.
Organism-specific databases
CTD	192309.
ZFIN	ZDB-GENE-020416-3. tpi1a.
Phylogenomic databases
eggNOG	fiNOG12007.
HOGENOM	HBG708281.
HOVERGEN	Q1MTI4.
InParanoid	Q1MTI4.
OMA	HSERANE.
OrthoDB	EOG9S4S28.
PhylomeDB	Q1MTI4.
Gene expression databases
ArrayExpress	Q1MTI4.
Bgee	Q1MTI4.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPISA_DANRE

Accession

Primary (citable) accession number: Q1MTI4
Secondary accession number(s): Q7ZWB0, Q90XF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	May 30, 2006
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project