Reviewed, UniProtKB/Swiss-Prot Q1MTD3 (MCES_DANRE)

Names and origin

Protein names

Recommended name:
    mRNA cap guanine-N7 methyltransferase
    EC=2.1.1.56
Alternative name(s):
    mRNA (guanine-N(7)-)-methyltransferase
    RG7MT1
    mRNA cap methyltransferase

Gene names

Name:	rnmt
ORF Names:	si:ch211-105d11.3

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC By similarity.
Catalytic activity	S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
Subcellular location	NucleusBy similarity.
Sequence similarities	Belongs to the mRNA cap methyltransferase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	mRNA capping Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	RNA binding Inferred from sequence or structural similarity. Source: UniProtKB mRNA (guanine-N7-)-methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	mRNA cap guanine-N7 methyltransferase		PRO_0000248325
Regions
	Region	100 – 104	5	mRNA cap-binding By similarity
	Region	185 – 186	2	S-adenosyl-L-methionine-binding By similarity
	Region	208 – 210	3	S-adenosyl-L-methionine-binding By similarity
Sites
	Binding site	104	1	S-adenosyl-L-methionine By similarity
	Binding site	128	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	137	1	mRNA cap By similarity
	Binding site	150	1	S-adenosyl-L-methionine By similarity
	Binding site	162	1	mRNA cap By similarity
	Binding site	391	1	mRNA cap By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1MTD3-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 271B592DBCF1663B	FASTA	400	45,961
	10 20 30 40 50 60 MMAQNSRLFE MDSPYADAKV DKDSIGSSFD SSQTSTSSSS SSVKRRRDGD EEDDDHSPSK 70 80 90 100 110 120 KLVTEDSLHS QKVATHYNKI KECGLAERNK SRIVYMRNFN NWLKSVLIAE ILDKVRQKRR 130 140 150 160 170 180 EVTVLDLGCG KGGDLLKWKK GRIDKLVCAD IAAVSIEQCQ QRYNDVRRRG HPNDRTFSAE 190 200 210 220 230 240 FITADCSREL LSEKLQDPEL QFDVCSCQFV YHYSFESESQ ADTMLRNACE RLRPGGFFIG 250 260 270 280 290 300 TTPDAYELVK RLEESDSNSF GNEVFSVTFQ KKGEYPLFGC QYDFSLEGVV NVPEFLVYFP 310 320 330 340 350 360 LFVEMAKKYN MRLVYKKTFK EFFEEKVKDG KNKDLMQWMQ ALEQYPPDER GQLSSSGPGE 370 380 390 400 YDHAKRKAAD PAVRRPLGTL SKSEWEATSI YLVYVFEKMS

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL928820 Genomic DNA. Translation: CAK03687.1.
RefSeq	NP_001038465.1. XP_001923422.1.
UniGene	Dr.68392
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSDARG00000061926. Danio rerio. [Contig view] ENSDARG00000070553. Danio rerio. [Contig view]
GeneID	100000432. 562801.
KEGG	dre:100000432. dre:562801.
NMPDR	fig|7955.3.peg.10345.
Organism-specific databases
ZFIN	ZDB-GENE-041008-25. si:ch211-105d11.3.
Phylogenomic databases
HOVERGEN	Q1MTD3.
Family and domain databases
InterPro	IPR016899. mRNA_G-N7_MeTrfase. IPR004971. Pox_MCEL. [Graphical view]
Pfam	PF03291. Pox_MCEL. 1 hit. [Graphical view]
PIRSF	PIRSF028762. ABD1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCES_DANRE

Accession

Primary (citable) accession number: Q1MTD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	May 30, 2006
Last modified:	September 2, 2008
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project