Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

mRNA cap guanine-N7 methyltransferase

Gene

rnmt

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei128 – 1281S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Sitei131 – 1311mRNA cap bindingPROSITE-ProRule annotation
Sitei137 – 1371mRNA cap bindingPROSITE-ProRule annotation
Binding sitei150 – 1501S-adenosyl-L-methioninePROSITE-ProRule annotation
Sitei162 – 1621mRNA cap bindingPROSITE-ProRule annotation
Binding sitei212 – 2121mRNA capPROSITE-ProRule annotation
Binding sitei294 – 2941mRNA capPROSITE-ProRule annotation
Binding sitei391 – 3911mRNA capPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-DRE-72086. mRNA Capping.
R-DRE-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA cap guanine-N7 methyltransferase (EC:2.1.1.56)
Alternative name(s):
RG7MT1
mRNA (guanine-N(7)-)-methyltransferase
mRNA cap methyltransferase
Gene namesi
Name:rnmt
ORF Names:si:ch211-105d11.3
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 19

Organism-specific databases

ZFINiZDB-GENE-041008-25. rnmt.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400mRNA cap guanine-N7 methyltransferasePRO_0000248325Add
BLAST

Proteomic databases

PaxDbiQ1MTD3.
PRIDEiQ1MTD3.

Expressioni

Gene expression databases

BgeeiENSDARG00000070553.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000083350.

Structurei

3D structure databases

ProteinModelPortaliQ1MTD3.
SMRiQ1MTD3. Positions 93-398.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 400349mRNA cap 0 methyltransferasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni100 – 1012mRNA cap bindingPROSITE-ProRule annotation
Regioni185 – 1862S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
Regioni208 – 2103S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.PROSITE-ProRule annotation
Contains 1 mRNA cap 0 methyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1975. Eukaryota.
ENOG410Y7HG. LUCA.
GeneTreeiENSGT00390000002368.
HOGENOMiHOG000242614.
HOVERGENiHBG081963.
InParanoidiQ1MTD3.
KOiK00565.
OMAiCGQVDTP.
OrthoDBiEOG091G0C9Y.
PhylomeDBiQ1MTD3.
TreeFamiTF314347.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR016899. mRNA_G-N7_MeTrfase.
IPR004971. mRNA_G-N7_MeTrfase_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF03291. Pox_MCEL. 1 hit.
[Graphical view]
PIRSFiPIRSF028762. ABD1. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51562. RNA_CAP0_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1MTD3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMAQNSRLFE MDSPYADAKV DKDSIGSSFD SSQTSTSSSS SSVKRRRDGD
60 70 80 90 100
EEDDDHSPSK KLVTEDSLHS QKVATHYNKI KECGLAERNK SRIVYMRNFN
110 120 130 140 150
NWLKSVLIAE ILDKVRQKRR EVTVLDLGCG KGGDLLKWKK GRIDKLVCAD
160 170 180 190 200
IAAVSIEQCQ QRYNDVRRRG HPNDRTFSAE FITADCSREL LSEKLQDPEL
210 220 230 240 250
QFDVCSCQFV YHYSFESESQ ADTMLRNACE RLRPGGFFIG TTPDAYELVK
260 270 280 290 300
RLEESDSNSF GNEVFSVTFQ KKGEYPLFGC QYDFSLEGVV NVPEFLVYFP
310 320 330 340 350
LFVEMAKKYN MRLVYKKTFK EFFEEKVKDG KNKDLMQWMQ ALEQYPPDER
360 370 380 390 400
GQLSSSGPGE YDHAKRKAAD PAVRRPLGTL SKSEWEATSI YLVYVFEKMS
Length:400
Mass (Da):45,961
Last modified:May 30, 2006 - v1
Checksum:i271B592DBCF1663B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL928820 Genomic DNA. Translation: CAK03687.1.
RefSeqiNP_001038465.1. NM_001045000.1.
UniGeneiDr.68392.

Genome annotation databases

EnsembliENSDART00000088917; ENSDARP00000083350; ENSDARG00000070553.
GeneIDi562801.
KEGGidre:562801.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL928820 Genomic DNA. Translation: CAK03687.1.
RefSeqiNP_001038465.1. NM_001045000.1.
UniGeneiDr.68392.

3D structure databases

ProteinModelPortaliQ1MTD3.
SMRiQ1MTD3. Positions 93-398.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000083350.

Proteomic databases

PaxDbiQ1MTD3.
PRIDEiQ1MTD3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000088917; ENSDARP00000083350; ENSDARG00000070553.
GeneIDi562801.
KEGGidre:562801.

Organism-specific databases

CTDi8731.
ZFINiZDB-GENE-041008-25. rnmt.

Phylogenomic databases

eggNOGiKOG1975. Eukaryota.
ENOG410Y7HG. LUCA.
GeneTreeiENSGT00390000002368.
HOGENOMiHOG000242614.
HOVERGENiHBG081963.
InParanoidiQ1MTD3.
KOiK00565.
OMAiCGQVDTP.
OrthoDBiEOG091G0C9Y.
PhylomeDBiQ1MTD3.
TreeFamiTF314347.

Enzyme and pathway databases

ReactomeiR-DRE-72086. mRNA Capping.
R-DRE-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

PROiQ1MTD3.

Gene expression databases

BgeeiENSDARG00000070553.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR016899. mRNA_G-N7_MeTrfase.
IPR004971. mRNA_G-N7_MeTrfase_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF03291. Pox_MCEL. 1 hit.
[Graphical view]
PIRSFiPIRSF028762. ABD1. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51562. RNA_CAP0_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCES_DANRE
AccessioniPrimary (citable) accession number: Q1MTD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: May 30, 2006
Last modified: September 7, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.