Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

mRNA cap guanine-N7 methyltransferase

Gene

rnmt

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the mRNA-capping methyltransferase RNMT:RAM/FAM103A1 complex that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.By similarity

Catalytic activityi

S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.PROSITE-ProRule annotationBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei128S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation1
Sitei131mRNA cap bindingPROSITE-ProRule annotation1
Sitei137mRNA cap bindingPROSITE-ProRule annotation1
Binding sitei150S-adenosyl-L-methioninePROSITE-ProRule annotation1
Sitei162mRNA cap bindingPROSITE-ProRule annotation1
Binding sitei185S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei208S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei212mRNA capPROSITE-ProRule annotation1
Binding sitei213S-adenosyl-L-methionineBy similarity1
Binding sitei294mRNA capPROSITE-ProRule annotation1
Binding sitei391mRNA capPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processmRNA capping, mRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-DRE-72086. mRNA Capping.
R-DRE-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA cap guanine-N7 methyltransferase (EC:2.1.1.56By similarity)
Alternative name(s):
RG7MT1
mRNA (guanine-N(7)-)-methyltransferase
mRNA cap methyltransferase
Gene namesi
Name:rnmt
ORF Names:si:ch211-105d11.3
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 19

Organism-specific databases

ZFINiZDB-GENE-041008-25. rnmt.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002483251 – 400mRNA cap guanine-N7 methyltransferaseAdd BLAST400

Proteomic databases

PaxDbiQ1MTD3.
PRIDEiQ1MTD3.

Expressioni

Gene expression databases

BgeeiENSDARG00000070553.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000083350.

Structurei

3D structure databases

ProteinModelPortaliQ1MTD3.
SMRiQ1MTD3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 400mRNA cap 0 methyltransferasePROSITE-ProRule annotationAdd BLAST349

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 101mRNA cap bindingPROSITE-ProRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1975. Eukaryota.
ENOG410Y7HG. LUCA.
GeneTreeiENSGT00390000002368.
HOGENOMiHOG000242614.
HOVERGENiHBG081963.
InParanoidiQ1MTD3.
KOiK00565.
OMAiLNLVSCQ.
OrthoDBiEOG091G0C9Y.
PhylomeDBiQ1MTD3.
TreeFamiTF314347.

Family and domain databases

InterProiView protein in InterPro
IPR016899. mRNA_G-N7_MeTrfase.
IPR004971. mRNA_G-N7_MeTrfase_dom.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF03291. Pox_MCEL. 1 hit.
PIRSFiPIRSF028762. ABD1. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiView protein in PROSITE
PS51562. RNA_CAP0_MT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1MTD3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMAQNSRLFE MDSPYADAKV DKDSIGSSFD SSQTSTSSSS SSVKRRRDGD
60 70 80 90 100
EEDDDHSPSK KLVTEDSLHS QKVATHYNKI KECGLAERNK SRIVYMRNFN
110 120 130 140 150
NWLKSVLIAE ILDKVRQKRR EVTVLDLGCG KGGDLLKWKK GRIDKLVCAD
160 170 180 190 200
IAAVSIEQCQ QRYNDVRRRG HPNDRTFSAE FITADCSREL LSEKLQDPEL
210 220 230 240 250
QFDVCSCQFV YHYSFESESQ ADTMLRNACE RLRPGGFFIG TTPDAYELVK
260 270 280 290 300
RLEESDSNSF GNEVFSVTFQ KKGEYPLFGC QYDFSLEGVV NVPEFLVYFP
310 320 330 340 350
LFVEMAKKYN MRLVYKKTFK EFFEEKVKDG KNKDLMQWMQ ALEQYPPDER
360 370 380 390 400
GQLSSSGPGE YDHAKRKAAD PAVRRPLGTL SKSEWEATSI YLVYVFEKMS
Length:400
Mass (Da):45,961
Last modified:May 30, 2006 - v1
Checksum:i271B592DBCF1663B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL928820 Genomic DNA. Translation: CAK03687.1.
RefSeqiNP_001038465.1. NM_001045000.1.
UniGeneiDr.68392.

Genome annotation databases

EnsembliENSDART00000088917; ENSDARP00000083350; ENSDARG00000070553.
GeneIDi562801.
KEGGidre:562801.

Similar proteinsi

Entry informationi

Entry nameiMCES_DANRE
AccessioniPrimary (citable) accession number: Q1MTD3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: May 30, 2006
Last modified: August 30, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families