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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfp

Organism
Lawsonia intracellularis (strain PHE/MN1-00)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei84 – 841ATP; via amide nitrogenUniRule annotation
Metal bindingi176 – 1761Magnesium; catalyticUniRule annotation
Sitei177 – 1771Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei206 – 2061Proton acceptorUniRule annotation
Binding sitei305 – 3051SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi150 – 1512ATPUniRule annotation
Nucleotide bindingi175 – 1784ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciLINT363253:GH6E-54-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfpImported
Synonyms:pfkAUniRule annotation
Ordered Locus Names:LI0052Imported
OrganismiLawsonia intracellularis (strain PHE/MN1-00)Imported
Taxonomic identifieri363253 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeLawsonia
ProteomesiUP000002430 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi363253.LI0052.

Structurei

3D structure databases

ProteinModelPortaliQ1MSB7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni204 – 2063Substrate bindingUniRule annotation
Regioni249 – 2513Substrate bindingUniRule annotation
Regioni357 – 3604Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
KOiK00850.
OMAiFGFQTAF.
OrthoDBiEOG6T7N42.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1MSB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNTHYSSLS VTLLPIKTLG EAAIPSPLPY CDIKKNEYIH LCLNQEQIED
60 70 80 90 100
LGSSPTLSLE LAGPRQDLYF EPQTTRCAIV TCGGLCPGLN DVIRAIVLEA
110 120 130 140 150
HYAYSVASVI GIRYGLEGFI PKFNHTPIEL TPDVVKNIHC FGGTILGSSR
160 170 180 190 200
GPQQPEEIVA SLVHHNINIL FVIGGDGSMK AANSITKEIH QHNLRISVIG
210 220 230 240 250
IPKTIDNDIN FIPKSFGFDT AVEKATEAIG CAHVEASGAK NGIGIVKLMG
260 270 280 290 300
RESGFIAAQS ALALKEANFV LIPEVQFTLH GNGGLLPALE KRLSERSHAV
310 320 330 340 350
IIVAEGAGQH LLDSQDETDP SGNPVLADIS TLLKNSIKDY FSKKDIPIIL
360 370 380 390 400
KLIDPSYIIR SVPANANDRV YCGFLGQYAV HAAMAGKTNM VVANIMDNYV
410 420 430 440 450
HIPLNMVIKK RRKLNTKSGL WRAVLESTGQ GRLTGMLPHC NPLTNEENEH

TKVII
Length:455
Mass (Da):49,345
Last modified:May 30, 2006 - v1
Checksum:iBE35B1DF19116242
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180252 Genomic DNA. Translation: CAJ54108.1.
RefSeqiWP_011526135.1. NC_008011.1.
YP_594430.1. NC_008011.1.

Genome annotation databases

KEGGilip:LI0052.
PATRICi22303411. VBILawInt40445_0059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180252 Genomic DNA. Translation: CAJ54108.1.
RefSeqiWP_011526135.1. NC_008011.1.
YP_594430.1. NC_008011.1.

3D structure databases

ProteinModelPortaliQ1MSB7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi363253.LI0052.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGilip:LI0052.
PATRICi22303411. VBILawInt40445_0059.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
KOiK00850.
OMAiFGFQTAF.
OrthoDBiEOG6T7N42.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciLINT363253:GH6E-54-MONOMER.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Lawsonia intracellularis: the causative agent of proliferative enteropathy."
    Kaur K., Zhang Q., Beckler D., Munir S., Li L., Kinsley K., Herron L., Peterson A., May B., Singh S., Gebhart C., Kapur V.
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PHE/MN1-00Imported.

Entry informationi

Entry nameiQ1MSB7_LAWIP
AccessioniPrimary (citable) accession number: Q1MSB7
Entry historyi
Integrated into UniProtKB/TrEMBL: May 30, 2006
Last sequence update: May 30, 2006
Last modified: May 27, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.