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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Lawsonia intracellularis (strain PHE/MN1-00)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei590 – 5901ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLINT363253:GH6E-159-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:LI0152
OrganismiLawsonia intracellularis (strain PHE/MN1-00)
Taxonomic identifieri363253 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeLawsonia
ProteomesiUP000002430: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 835835Leucine--tRNA ligasePRO_0000334768Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi363253.LI0152.

Structurei

3D structure databases

ProteinModelPortaliQ1MS17.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi44 – 5411"HIGH" regionAdd
BLAST
Motifi587 – 5915"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1MS17-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIMHSNYDPN IIEKKWQRYW EEQHAYACDI DPNKKKYYVL EMWPYPSGNI
60 70 80 90 100
HMGHVRNYSI GDVIARIKRM QGYNVLHPMG WDAFGLPAEL AAIKHHTQPA
110 120 130 140 150
DWTYTNINEM RAQLKRLGYS YDWQREIATC DPDYYQWEQL FFVDCFEKGL
160 170 180 190 200
VYRKKAPQNW CPSCNTVLAN EQVIEGSCWR CDTSIIQKEL TQWFLKITDY
210 220 230 240 250
AEELLDDLTT LKYSWPDRVI TMQENWLGKS TGVEISFPIE SSQDFITIFT
260 270 280 290 300
TRPDTIFGAT AIILAPEHPI LETLLNDTPQ KEKLYNVIEK MRNMDRLERQ
310 320 330 340 350
SNLLTKEGIF IDRYCINPCS GDKIPIWIGN FVLADYGTGA IMAVPAHDQR
360 370 380 390 400
DFDFCKKYNL PISIVIQPDE LQINEHTIVE PWTGLGTLIH SGQFTGLTNK
410 420 430 440 450
EAKEKITQYL EKIGKATRVV SWRLRDWNIS RQRYWGTPIP IIYCKNCGVV
460 470 480 490 500
SVPKNELPVI LPTNITINED GSSPLPQESS FIDCMCPQCG GIAKRETDTM
510 520 530 540 550
DTFVDSSWYF ARFVDPKNTK QPFDTSIAKY WLNVDQYIGG VEHAILHLLY
560 570 580 590 600
SRFFTKLLRD LGYLPSNINE PFAKLLTQGM VIKDGSKMSK SKGNIVDPNQ
610 620 630 640 650
MINIYGADTI RLFCLFAAPP ERDFDWSDSG IEGSYRFLHR VWRLFIDVQP
660 670 680 690 700
HIHTTHAGNS TVIETTTALT KDVKRKEHIT IKKVSEDIEN KYQFNTAIAS
710 720 730 740 750
IMEFVNTLYL SKNELILTVE GKNILSSAIA TVLTLLSPMT PHICEELWSY
760 770 780 790 800
LGHSTSISLE PWPKWDPQAL LKDVVTIVVQ INGKVRNKFE VPMDLSKEEL
810 820 830
EHIIFNDTKI TQYLEGKTIQ KHIIIPNKII NIVTT
Length:835
Mass (Da):96,315
Last modified:May 30, 2006 - v1
Checksum:i98E033A6AB677868
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180252 Genomic DNA. Translation: CAJ54208.1.
RefSeqiWP_011526235.1. NC_008011.1.
YP_594530.1. NC_008011.1.

Genome annotation databases

GeneIDi4059645.
KEGGilip:LI0152.
PATRICi22303643. VBILawInt40445_0170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180252 Genomic DNA. Translation: CAJ54208.1.
RefSeqiWP_011526235.1. NC_008011.1.
YP_594530.1. NC_008011.1.

3D structure databases

ProteinModelPortaliQ1MS17.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi363253.LI0152.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4059645.
KEGGilip:LI0152.
PATRICi22303643. VBILawInt40445_0170.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciLINT363253:GH6E-159-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Lawsonia intracellularis: the causative agent of proliferative enteropathy."
    Kaur K., Zhang Q., Beckler D., Munir S., Li L., Kinsley K., Herron L., Peterson A., May B., Singh S., Gebhart C., Kapur V.
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PHE/MN1-00.

Entry informationi

Entry nameiSYL_LAWIP
AccessioniPrimary (citable) accession number: Q1MS17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 30, 2006
Last modified: February 4, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.