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Q1MS17 (SYL_LAWIP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:LI0152
OrganismLawsonia intracellularis (strain PHE/MN1-00) [Complete proteome] [HAMAP]
Taxonomic identifier363253 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeLawsonia

Protein attributes

Sequence length835 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 835835Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334768

Regions

Motif44 – 5411"HIGH" region HAMAP-Rule MF_00049
Motif587 – 5915"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5901ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1MS17 [UniParc].

Last modified May 30, 2006. Version 1.
Checksum: 98E033A6AB677868

FASTA83596,315
        10         20         30         40         50         60 
MIMHSNYDPN IIEKKWQRYW EEQHAYACDI DPNKKKYYVL EMWPYPSGNI HMGHVRNYSI 

        70         80         90        100        110        120 
GDVIARIKRM QGYNVLHPMG WDAFGLPAEL AAIKHHTQPA DWTYTNINEM RAQLKRLGYS 

       130        140        150        160        170        180 
YDWQREIATC DPDYYQWEQL FFVDCFEKGL VYRKKAPQNW CPSCNTVLAN EQVIEGSCWR 

       190        200        210        220        230        240 
CDTSIIQKEL TQWFLKITDY AEELLDDLTT LKYSWPDRVI TMQENWLGKS TGVEISFPIE 

       250        260        270        280        290        300 
SSQDFITIFT TRPDTIFGAT AIILAPEHPI LETLLNDTPQ KEKLYNVIEK MRNMDRLERQ 

       310        320        330        340        350        360 
SNLLTKEGIF IDRYCINPCS GDKIPIWIGN FVLADYGTGA IMAVPAHDQR DFDFCKKYNL 

       370        380        390        400        410        420 
PISIVIQPDE LQINEHTIVE PWTGLGTLIH SGQFTGLTNK EAKEKITQYL EKIGKATRVV 

       430        440        450        460        470        480 
SWRLRDWNIS RQRYWGTPIP IIYCKNCGVV SVPKNELPVI LPTNITINED GSSPLPQESS 

       490        500        510        520        530        540 
FIDCMCPQCG GIAKRETDTM DTFVDSSWYF ARFVDPKNTK QPFDTSIAKY WLNVDQYIGG 

       550        560        570        580        590        600 
VEHAILHLLY SRFFTKLLRD LGYLPSNINE PFAKLLTQGM VIKDGSKMSK SKGNIVDPNQ 

       610        620        630        640        650        660 
MINIYGADTI RLFCLFAAPP ERDFDWSDSG IEGSYRFLHR VWRLFIDVQP HIHTTHAGNS 

       670        680        690        700        710        720 
TVIETTTALT KDVKRKEHIT IKKVSEDIEN KYQFNTAIAS IMEFVNTLYL SKNELILTVE 

       730        740        750        760        770        780 
GKNILSSAIA TVLTLLSPMT PHICEELWSY LGHSTSISLE PWPKWDPQAL LKDVVTIVVQ 

       790        800        810        820        830 
INGKVRNKFE VPMDLSKEEL EHIIFNDTKI TQYLEGKTIQ KHIIIPNKII NIVTT 

« Hide

References

[1]"The complete genome sequence of Lawsonia intracellularis: the causative agent of proliferative enteropathy."
Kaur K., Zhang Q., Beckler D., Munir S., Li L., Kinsley K., Herron L., Peterson A., May B., Singh S., Gebhart C., Kapur V.
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PHE/MN1-00.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM180252 Genomic DNA. Translation: CAJ54208.1.
RefSeqYP_594530.1. NC_008011.1.

3D structure databases

ProteinModelPortalQ1MS17.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING363253.LI0152.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4059645.
KEGGlip:LI0152.
PATRIC22303643. VBILawInt40445_0170.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycLINT363253:GH6E-159-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LAWIP
AccessionPrimary (citable) accession number: Q1MS17
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 30, 2006
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries