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Q1MR76 (ISPDF_LAWIP) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional enzyme IspD/IspF

Including the following 2 domains:

  1. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
    EC=2.7.7.60
    Alternative name(s):
    4-diphosphocytidyl-2C-methyl-D-erythritol synthase
    MEP cytidylyltransferase
    Short name=MCT
  2. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
    Short name=MECDP-synthase
    Short name=MECPS
    EC=4.6.1.12
Gene names
Name:ispDF
Ordered Locus Names:LI0446
OrganismLawsonia intracellularis (strain PHE/MN1-00) [Complete proteome] [HAMAP]
Taxonomic identifier363253 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Lawsonia

Protein attributes

Sequence length399 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (IspF) By similarity. HAMAP MF_01520

Catalytic activity

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520

2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520

Cofactor

Divalent metal cations By similarity. HAMAP MF_01520

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.

Sequence similarities

In the N-terminal section; belongs to the IspD family.

In the C-terminal section; belongs to the IspF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 399399Bifunctional enzyme IspD/IspF HAMAP MF_01520
PRO_0000296748

Regions

Region1 – 2352352-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
Region236 – 3991642-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520

Sites

Metal binding2421Divalent metal cation By similarity
Metal binding2441Divalent metal cation By similarity
Metal binding2831Divalent metal cation By similarity
Site151Transition state stabilizer By similarity
Site241Transition state stabilizer By similarity
Site1601Positions MEP for the nucleophilic attack By similarity
Site2161Positions MEP for the nucleophilic attack By similarity
Site2751Transition state stabilizer By similarity
Site3741Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1MR76 [UniParc].

Last modified May 30, 2006. Version 1.
Checksum: 5DA3E66FA1C0378C

FASTA39943,819
        10         20         30         40         50         60 
METWALILAA GQGSRLLSTI GIAKQFFVWK GIPLYWQSVL QFMHCARIRG VVLVFPSEVK 

        70         80         90        100        110        120 
DNEEKVVDRL AAQYDLRLPY IVISGGKLRQ DSVKNALNTL PKNCSHVLIH DAARPFISPK 

       130        140        150        160        170        180 
LINNVIEVLE AGAVGVVPGI SVTDTIKQVI QGEVIVTRPR EQLIAVQTPQ GFHLKTIVEG 

       190        200        210        220        230        240 
HNRATLEKWT VTDDASLLEL CGHTVQVING EIENRKISFP QDLLYMVEQP KTTVPIVGYG 

       250        260        270        280        290        300 
YDVHKYVTED QINQPIRSMR LGGISIPNAP NVVAHSDGDV LLHALMDALL GCIGGGDIGL 

       310        320        330        340        350        360 
HFPDSDKRYD GINSAILLDH VLTKVLESSI KIVHMDATIV AQLPKISQYR EAIRSNLSRL 

       370        380        390 
LDLDLANVNI KATTEEGLGF TGECKGIKAI VIVTAIRIS

« Hide

References

[1]"The complete genome sequence of Lawsonia intracellularis: the causative agent of proliferative enteropathy."
Kaur K., Zhang Q., Beckler D., Munir S., Li L., Kinsley K., Herron L., Peterson A., May B., Singh S., Gebhart C., Kapur V.
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PHE/MN1-00.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM180252 Genomic DNA. Translation: CAJ54500.1.
RefSeqYP_594822.1. NC_008011.1.

3D structure databases

ProteinModelPortalQ1MR76.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ1MR76.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4060193.
GenomeReviewsGene locus LI0446 in contig AM180252_GR.
KEGGlip:LI0446.
PATRIC22304301. VBILawInt40445_0490.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1211.
HOGENOMHBG672839.
OMAIVLIHDA.
PhylomeDBQ1MR76.
ProtClustDBCLSK705254.

Enzyme and pathway databases

BioCycLINT363253:LI0446-MONOMER.

Family and domain databases

HAMAPMF_01520. IspDF.
[Tree]
InterProIPR023423. IpsF_dom.
IPR001228. ISPD_synthase.
IPR018294. ISPD_synthase_CS.
IPR003526. MECDP_synthase.
IPR020555. MECDP_synthase_CS.
[Graphical view]
Gene3DG3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.
KOK12506.
PfamPF01128. IspD. 1 hit.
PF02542. YgbB. 1 hit.
[Graphical view]
SUPFAMSSF69765. YgbB_synth. 1 hit.
TIGRFAMsTIGR00453. IspD. 1 hit.
TIGR00151. IspF. 1 hit.
PROSITEPS01295. ISPD. 1 hit.
PS01350. ISPF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameISPDF_LAWIP
AccessionPrimary (citable) accession number: Q1MR76
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: May 30, 2006
Last modified: January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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