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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Lawsonia intracellularis (strain PHE/MN1-00)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99ZincUniRule annotation1
Metal bindingi101ZincUniRule annotation1
Metal bindingi126ZincUniRule annotation1
Metal bindingi128ZincUniRule annotation1
Binding sitei239ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLINT363253:G1GJC-512-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:LI0462
OrganismiLawsonia intracellularis (strain PHE/MN1-00)
Taxonomic identifieri363253 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeLawsonia
Proteomesi
  • UP000002430 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000019161 – 465Glutamate--tRNA ligaseAdd BLAST465

Proteomic databases

PRIDEiQ1MR60

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi363253.LI0462

Structurei

3D structure databases

ProteinModelPortaliQ1MR60
SMRiQ1MR60
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 20"HIGH" regionUniRule annotationAdd BLAST11
Motifi236 – 240"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OMAiAYYAFDT

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q1MR60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKVVTRFAP SPTGQLHIGG ARTALFSWLL ARHFDGIFHL RIEDTDLERS
60 70 80 90 100
KQEYTDTILE SMSWLGLNWD GEIVYQSKRN ERYHEVIDIL LQTGHAYWCH
110 120 130 140 150
CSIEQIEKMR EEARQKGEKP RYNGCCREKK LSANPSGVVR LKVPSAGQVT
160 170 180 190 200
FNDLVKGQVT VQNTELDDMV LQRSDGTPTY QLAVVVDDHD MQVTHIIRGD
210 220 230 240 250
DHVSNTPKQI LIYNALGWEI PIFGHIPMIL GSDRQKLSKR HGARAVIEYQ
260 270 280 290 300
EDGFLPEAML NYLVRLGWSY GDQEIFTLQE LISFFDGTNL HSAPAAFNIE
310 320 330 340 350
KLLWLNAHYL RRLPVSQIAN LMLPFVRKEG FTHITKERIE AVLPLYVERA
360 370 380 390 400
DTLKELVYLM KPVLISASDL TYKEDDIKKI VTNESKEHLD RLRNLLEAEI
410 420 430 440 450
NFEPLYIEQL IHEYITSNNL KFKEIGPILR LAVSGVLGGP SLQELIVVIG
460
KKEVLARLDK LRFQL
Length:465
Mass (Da):53,398
Last modified:May 30, 2006 - v1
Checksum:i069CC723DBC98F2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180252 Genomic DNA Translation: CAJ54516.1
RefSeqiWP_011526546.1, NC_008011.1

Genome annotation databases

KEGGilip:LI0462

Similar proteinsi

Entry informationi

Entry nameiSYE_LAWIP
AccessioniPrimary (citable) accession number: Q1MR60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 30, 2006
Last modified: May 23, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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