Reviewed, UniProtKB/Swiss-Prot Q1MQ25 (TRMFO_LAWIP)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase
    Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Ordered Locus Names:	LI0848

Organism

Lawsonia intracellularis (strain PHE/MN1-00) [Complete proteome] [HAMAP]

Taxonomic identifier

363253 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Lawsonia

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC tRNA (uracil-5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO HAMAP MF_01037		PRO_0000346349
Regions
	Nucleotide binding	10 – 15	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1MQ25-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: AF1D0E005E6956A3	FASTA	445	49,746
	10 20 30 40 50 60 MNIHNIAIIG GGLSGCECAL TLAKFGFSVT LFEQKPQLFS PAHNTPLLAE LVCSNSLRSN 70 80 90 100 110 120 ELTTGIGLLK QELRELNSPL MAIADTCRVP AGKALAVDRE LFSKQVTQLI ESHPKIHLIR 130 140 150 160 170 180 EEVSSLSTSF LEKYDRIIVA TGPLASPNIS NSLSTLIGDK YLYFYDAIAP IVTADSIDMS 190 200 210 220 230 240 IAFWGSRYEE QGEGDYLNCP MSYEEYQIFY SSLLKGEKVT NSKVEKEIHF EGCMPIEALA 250 260 270 280 290 300 ERGEKTLLFG PFKPVGLINP HTGLRPYAVL QLRPENLNKS MLNLVGCQTK LTYPAQDTIF 310 320 330 340 350 360 RLVPGLSNVE FVRFGSMHRN TYINSPKILT DQLALRNFPC IHLAGQITGV EGYVESIACG 370 380 390 400 410 420 LWVSILLLAL SKDKKILRPP KTCALGGLLE HISCPSKQFQ PSNIHFGLVP EPTEKIKKKE 430 440 RKEWYAQRAR IDFYHWLNNE LIHIM

References

[1]

"The complete genome sequence of Lawsonia intracellularis: the causative agent of proliferative enteropathy." Kaur K., Zhang Q., Beckler D., Munir S., Li L., Kinsley K., Herron L., Peterson A., May B., Singh S., Gebhart C., Kapur V. Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM180252 Genomic DNA. Translation: CAJ54902.1.
RefSeq	YP_595223.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4060322.
GenomeReviews	Gene locus LI0848 in contig AM180252_GR.
KEGG	lip:LI0848.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1MQ25.
OMA	Q1MQ25. MKPVGLT.
Family and domain databases
HAMAP	MF_01037. [Tree]
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004417. Gid. IPR002218. GIDA-rel. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_LAWIP

Accession

Primary (citable) accession number: Q1MQ25

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 30, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)