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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Lawsonia intracellularis (strain PHE/MN1-00)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 15FADUniRule annotation6

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:LI0848
OrganismiLawsonia intracellularis (strain PHE/MN1-00)
Taxonomic identifieri363253 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeLawsonia
Proteomesi
  • UP000002430 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003463491 – 445Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOAdd BLAST445

Proteomic databases

PRIDEiQ1MQ25.

Interactioni

Protein-protein interaction databases

STRINGi363253.LI0848.

Structurei

3D structure databases

ProteinModelPortaliQ1MQ25.
SMRiQ1MQ25.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR016040. NAD(P)-bd_dom.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1MQ25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIHNIAIIG GGLSGCECAL TLAKFGFSVT LFEQKPQLFS PAHNTPLLAE
60 70 80 90 100
LVCSNSLRSN ELTTGIGLLK QELRELNSPL MAIADTCRVP AGKALAVDRE
110 120 130 140 150
LFSKQVTQLI ESHPKIHLIR EEVSSLSTSF LEKYDRIIVA TGPLASPNIS
160 170 180 190 200
NSLSTLIGDK YLYFYDAIAP IVTADSIDMS IAFWGSRYEE QGEGDYLNCP
210 220 230 240 250
MSYEEYQIFY SSLLKGEKVT NSKVEKEIHF EGCMPIEALA ERGEKTLLFG
260 270 280 290 300
PFKPVGLINP HTGLRPYAVL QLRPENLNKS MLNLVGCQTK LTYPAQDTIF
310 320 330 340 350
RLVPGLSNVE FVRFGSMHRN TYINSPKILT DQLALRNFPC IHLAGQITGV
360 370 380 390 400
EGYVESIACG LWVSILLLAL SKDKKILRPP KTCALGGLLE HISCPSKQFQ
410 420 430 440
PSNIHFGLVP EPTEKIKKKE RKEWYAQRAR IDFYHWLNNE LIHIM
Length:445
Mass (Da):49,746
Last modified:May 30, 2006 - v1
Checksum:iAF1D0E005E6956A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180252 Genomic DNA. Translation: CAJ54902.1.
RefSeqiWP_011526931.1. NC_008011.1.

Genome annotation databases

KEGGilip:LI0848.
PATRICi22305195. VBILawInt40445_0920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180252 Genomic DNA. Translation: CAJ54902.1.
RefSeqiWP_011526931.1. NC_008011.1.

3D structure databases

ProteinModelPortaliQ1MQ25.
SMRiQ1MQ25.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi363253.LI0848.

Proteomic databases

PRIDEiQ1MQ25.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGilip:LI0848.
PATRICi22305195. VBILawInt40445_0920.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR016040. NAD(P)-bd_dom.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMFO_LAWIP
AccessioniPrimary (citable) accession number: Q1MQ25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 30, 2006
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.