Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Lawsonia intracellularis (strain PHE/MN1-00)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781Charge relay systemUniRule annotation
Active sitei153 – 1531Charge relay systemUniRule annotation
Active sitei177 – 1771Acyl-ester intermediateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLINT363253:GH6E-918-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:LI0885
OrganismiLawsonia intracellularis (strain PHE/MN1-00)
Taxonomic identifieri363253 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeLawsonia
Proteomesi
  • UP000002430 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485Glutamyl-tRNA(Gln) amidotransferase subunit APRO_1000015851Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi363253.LI0885.

Structurei

3D structure databases

ProteinModelPortaliQ1MPY8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiHASPLEM.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1MPY8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSDIHLSLA QIHNLLISRE KSVEEVTQAC LDRIIATEPT INAFITICAE
60 70 80 90 100
EALSEAKKLD QSTPDPKKPL WGIPIAVKDN ILTKNIPTTA ASCMLKHFIP
110 120 130 140 150
QYDAFIIQQL KNAGAIIIGK TNLDEFAMGS STETSFFGPT YNPWNTKCVP
160 170 180 190 200
GGSSGGSAAS VAAFQCFSAI GTDTGGSIRQ PAALCGCIGL KPTYGRVSRY
210 220 230 240 250
GIIAYASSLD QAGPITRTVE DAAIMLSVLA KHDPQDTTSS FKATDNYYIN
260 270 280 290 300
LKKQDLTGIT IGIPKEFISE HIDPPILDIY QQAIEQAKEL GAKTIDLSLP
310 320 330 340 350
HATDHAIATY YIIATAEASS NLARFDGVRY GYRAKNSHTL EELYINSRTK
360 370 380 390 400
GFGEEVKRRI LLGTHVLSTD YYENYYHKAA QVRYLILQDF LSVFKKCDIL
410 420 430 440 450
LTPVSPITAW EIGSTIKKPI TIYHKDIFTV SLNLAGLPGL SIPAGLVKGL
460 470 480
PVGIQLIGQA FDESTLLSIG NILHKYLGPT SQPNL
Length:485
Mass (Da):52,760
Last modified:May 30, 2006 - v1
Checksum:i2DD52713E4ABCEFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180252 Genomic DNA. Translation: CAJ54939.1.
RefSeqiWP_011526968.1. NC_008011.1.

Genome annotation databases

KEGGilip:LI0885.
PATRICi22305281. VBILawInt40445_0961.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180252 Genomic DNA. Translation: CAJ54939.1.
RefSeqiWP_011526968.1. NC_008011.1.

3D structure databases

ProteinModelPortaliQ1MPY8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi363253.LI0885.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGilip:LI0885.
PATRICi22305281. VBILawInt40445_0961.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiHASPLEM.

Enzyme and pathway databases

BioCyciLINT363253:GH6E-918-MONOMER.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_LAWIP
AccessioniPrimary (citable) accession number: Q1MPY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 30, 2006
Last modified: September 7, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.