Q1MPN3 (CARA_LAWIP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbamoyl-phosphate synthase small chain
EC=6.3.5.5

Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	LI0990

Organism

Lawsonia intracellularis (strain PHE/MN1-00) [Complete proteome] [HAMAP]

Taxonomic identifier

363253 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Lawsonia

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the CarA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_1000138863
Regions
	Domain	189 – 376	188	Glutamine amidotransferase type-1
	Region	1 – 187	187	CPSase HAMAP MF_01209
Sites
	Active site	266	1	Nucleophile By similarity
	Active site	349	1	By similarity
	Active site	351	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1MPN3 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 1A50077C26121F80	FASTA	376	41,736
	10 20 30 40 50 60 MKALLALEDG FILEGQSING HCESSGEVIF NTGMTGYQEI LTDPSYYGQM VCMTWPLIGN 70 80 90 100 110 120 YGINKEDMES EKIHVSALIV KECCRNPSNW RSETSLPKFL QEYNIAGIEG IDTRALTRHI 130 140 150 160 170 180 RINGAMRGII STSITDPNDL ILRVKKVPSM EGQNYVTKVA PNSPWVLYGQ CVVPADIKED 190 200 210 220 230 240 GSFLWKQNKI PLIVYDYGIK WNIIRLLEGA GFDPLMVPPL FSLEQVKASG AKAIFLSNGP 250 260 270 280 290 300 GDPGTLIDEI QIIRELMEYY PIAGICLGHQ LLGHAVGGTT RKLTFGHHGS NHPIKNLATG 310 320 330 340 350 360 HIEISSQNHG FCVNFESNND IEVTHINLND NTLEGFIHKT KPILAVQHHP EASPGPMDSQ 370 YFFTRFKEVV LLKLGC

References

[1]

"The complete genome sequence of Lawsonia intracellularis: the causative agent of proliferative enteropathy." Kaur K., Zhang Q., Beckler D., Munir S., Li L., Kinsley K., Herron L., Peterson A., May B., Singh S., Gebhart C., Kapur V. Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PHE/MN1-00.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM180252 Genomic DNA. Translation: CAJ55044.1.
RefSeq	YP_595365.1. NC_008011.1.
3D structure databases
ProteinModelPortal	Q1MPN3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1MPN3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4060402.
GenomeReviews	Gene locus LI0990 in contig AM180252_GR.
KEGG	lip:LI0990.
PATRIC	22305521. VBILawInt40445_1077.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0505.
HOGENOM	HBG286341.
OMA	FTYPELG.
PhylomeDB	Q1MPN3.
ProtClustDB	PRK12564.
Enzyme and pathway databases
BioCyc	LINT363253:LI0990-MONOMER.
Family and domain databases
HAMAP	MF_01209. CPSase_S_chain. [Tree]
InterPro	IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR017926. GATASE_1. [Graphical view]
Gene3D	G3DSA:3.50.30.20. G3DSA:3.50.30.20. 1 hit.
KO	K01956.
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
SMART	SM01097. CPSase_sm_chain. 1 hit. [Graphical view]
SUPFAM	SSF52021. CP_synthsmall. 1 hit.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_LAWIP

Accession

Primary (citable) accession number: Q1MPN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 30, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program