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Protein

NAD kinase

Gene

nadK

Organism
Lawsonia intracellularis (strain PHE/MN1-00)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei64 – 641Proton acceptorUniRule annotation
Binding sitei149 – 1491NADUniRule annotation
Binding sitei166 – 1661NADUniRule annotation
Binding sitei168 – 1681NADUniRule annotation
Binding sitei176 – 1761NAD; via carbonyl oxygenUniRule annotation
Binding sitei238 – 2381NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 652NADUniRule annotation
Nucleotide bindingi138 – 1392NADUniRule annotation
Nucleotide bindingi179 – 1846NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciLINT363253:GH6E-1049-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:LI1009
OrganismiLawsonia intracellularis (strain PHE/MN1-00)
Taxonomic identifieri363253 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeLawsonia
ProteomesiUP000002430: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285NAD kinasePRO_1000059875Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi363253.LI1009.

Structurei

3D structure databases

ProteinModelPortaliQ1MPL4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiRIAHEIF.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1MPL4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLKHALIV YKVGNPQAEK TAHDIQHWFY NKNVTSNLFS SDIPESQLKQ
60 70 80 90 100
SLVHTQVAII LGGDGTFLSI SRNLIEKQIP VLGINFGQVG FLVEIHPENW
110 120 130 140 150
PQMLEQLYSH KLVLQKKIVL SWSIIRHNQV IKNGFAINDV VVGRGALARV
160 170 180 190 200
LAVDVSINKH HIGVIRSDGI LVSTPLGTSG YTISAHGPLV HPDVQALTLT
210 220 230 240 250
SVSTLFRSTP PLVLPLSTTI TLTPSPHAIE PFLTVDGQEG FVLKPNDSVG
260 270 280
IQGIKSGLLI YTTANYSYLQ HLQKKGLLSS TDFNL
Length:285
Mass (Da):31,242
Last modified:May 30, 2006 - v1
Checksum:i940D160C3526D938
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180252 Genomic DNA. Translation: CAJ55063.1.
RefSeqiYP_595384.1. NC_008011.1.

Genome annotation databases

GeneIDi4059621.
KEGGilip:LI1009.
PATRICi22305563. VBILawInt40445_1098.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM180252 Genomic DNA. Translation: CAJ55063.1.
RefSeqiYP_595384.1. NC_008011.1.

3D structure databases

ProteinModelPortaliQ1MPL4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi363253.LI1009.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4059621.
KEGGilip:LI1009.
PATRICi22305563. VBILawInt40445_1098.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiRIAHEIF.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciLINT363253:GH6E-1049-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Lawsonia intracellularis: the causative agent of proliferative enteropathy."
    Kaur K., Zhang Q., Beckler D., Munir S., Li L., Kinsley K., Herron L., Peterson A., May B., Singh S., Gebhart C., Kapur V.
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PHE/MN1-00.

Entry informationi

Entry nameiNADK_LAWIP
AccessioniPrimary (citable) accession number: Q1MPL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 30, 2006
Last modified: February 4, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.