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Q1MPH2

- SYI_LAWIP

UniProt

Q1MPH2 - SYI_LAWIP

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lawsonia intracellularis (strain PHE/MN1-00)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (30 May 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei566 – 5661Aminoacyl-adenylateUniRule annotation
    Binding sitei610 – 6101ATPUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation
    Metal bindingi925 – 9251ZincUniRule annotation
    Metal bindingi928 – 9281ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciLINT363253:GH6E-1093-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:LI1051
    OrganismiLawsonia intracellularis (strain PHE/MN1-00)
    Taxonomic identifieri363253 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeLawsonia
    ProteomesiUP000002430: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 937937Isoleucine--tRNA ligasePRO_1000070889Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi363253.LI1051.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1MPH2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi607 – 6115"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q1MPH2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDYKETLNL PNTTFPMKAN LVQREPEIIL WWEENAVYEK MLEASGAKGI    50
    FILHDGPPYA NGHIHLGTAL NKILKDIVIK SRNMQGYRSC YVPGWDCHGL 100
    PIELKVEQEL GKKKQEMPLS LIRNRCREYA EKFLDIQREE FKRLGVFGSW 150
    DHPYQTMDPI YESVITLELA RFVEKGSVIR SKKPIYWCYS CETALAEAEV 200
    EYADHTSSAI FVRFPIHDKR LHTIFPQADL TTTSIVIWTT TPWTLPSNMA 250
    IALNADFDYA LLQYKNEYII IASELVDICL KQFNWEDAKV IKVVQGKDLE 300
    GMKARHPLYD QESMIVLGDH VTLEAGTGCV HTAPGHGPED YEVALRYNLD 350
    VYSPLDDQGR YLDTVKFFAG LRVDQANPVV IQKLEEFHRI IQKNTIQHSY 400
    PHCWRCKSPV IFRATTQWFI SMEKNNLREQ SLKAIKKNIE WIPSWGEDRI 450
    YNMIASRPDW CISRQRIWGV PIVALICESC GEVWNDPSWM KKIAEFFAIH 500
    PRGCDYWYEA KLEDIVPVGL KCPHCEGEQW KRESDILDVW FDSGSSFAAV 550
    LEERPNLGFP ADLYLEGSDQ HRGWFHSSLL ISIGTRGVPP YHAVLTHGYV 600
    VDGDGRKMSK SMGNVTSPQE IISKFGVEIL RLWVSSVDYR EDVRISNEIL 650
    QRLVDAYRRI RNTCRYLLGN INDLTLDELV PVKEMESLDQ YILDVVATAY 700
    TEIQKSYISY DFHTVFHKLH NLCTTDLSAF YLDILKDRLY TSGVRSHKRK 750
    SAQTALFYIL HMLLRSMAPI LSFTAEEVYK YIPDTLKDDN VISVFMLPFF 800
    ETSSFLLDDR VRSYWETLLL IRAEVNQAIE PMRKKGEIGH SLDTHITLYV 850
    APELHTLLLE LNTDLCSLFI VSQLDIMPLS EASVDAAVSK IDGLAVAVNR 900
    AQGNKCQRCW MYKELGSNHQ YPTLCPRCTE VVENMKI 937
    Length:937
    Mass (Da):107,965
    Last modified:May 30, 2006 - v1
    Checksum:i5CCC1C52FF4081CF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM180252 Genomic DNA. Translation: CAJ55105.1.
    RefSeqiWP_011527134.1. NC_008011.1.
    YP_595426.1. NC_008011.1.

    Genome annotation databases

    GeneIDi4059667.
    KEGGilip:LI1051.
    PATRICi22305659. VBILawInt40445_1144.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM180252 Genomic DNA. Translation: CAJ55105.1 .
    RefSeqi WP_011527134.1. NC_008011.1.
    YP_595426.1. NC_008011.1.

    3D structure databases

    ProteinModelPortali Q1MPH2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 363253.LI1051.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4059667.
    KEGGi lip:LI1051.
    PATRICi 22305659. VBILawInt40445_1144.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci LINT363253:GH6E-1093-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Lawsonia intracellularis: the causative agent of proliferative enteropathy."
      Kaur K., Zhang Q., Beckler D., Munir S., Li L., Kinsley K., Herron L., Peterson A., May B., Singh S., Gebhart C., Kapur V.
      Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PHE/MN1-00.

    Entry informationi

    Entry nameiSYI_LAWIP
    AccessioniPrimary (citable) accession number: Q1MPH2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: May 30, 2006
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3