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Q1MPH2

- SYI_LAWIP

UniProt

Q1MPH2 - SYI_LAWIP

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lawsonia intracellularis (strain PHE/MN1-00)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei566 – 5661Aminoacyl-adenylateUniRule annotation
Binding sitei610 – 6101ATPUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation
Metal bindingi928 – 9281ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLINT363253:GH6E-1093-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LI1051
OrganismiLawsonia intracellularis (strain PHE/MN1-00)
Taxonomic identifieri363253 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeLawsonia
ProteomesiUP000002430: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 937937Isoleucine--tRNA ligasePRO_1000070889Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi363253.LI1051.

Structurei

3D structure databases

ProteinModelPortaliQ1MPH2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi607 – 6115"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1MPH2-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MTDYKETLNL PNTTFPMKAN LVQREPEIIL WWEENAVYEK MLEASGAKGI
60 70 80 90 100
FILHDGPPYA NGHIHLGTAL NKILKDIVIK SRNMQGYRSC YVPGWDCHGL
110 120 130 140 150
PIELKVEQEL GKKKQEMPLS LIRNRCREYA EKFLDIQREE FKRLGVFGSW
160 170 180 190 200
DHPYQTMDPI YESVITLELA RFVEKGSVIR SKKPIYWCYS CETALAEAEV
210 220 230 240 250
EYADHTSSAI FVRFPIHDKR LHTIFPQADL TTTSIVIWTT TPWTLPSNMA
260 270 280 290 300
IALNADFDYA LLQYKNEYII IASELVDICL KQFNWEDAKV IKVVQGKDLE
310 320 330 340 350
GMKARHPLYD QESMIVLGDH VTLEAGTGCV HTAPGHGPED YEVALRYNLD
360 370 380 390 400
VYSPLDDQGR YLDTVKFFAG LRVDQANPVV IQKLEEFHRI IQKNTIQHSY
410 420 430 440 450
PHCWRCKSPV IFRATTQWFI SMEKNNLREQ SLKAIKKNIE WIPSWGEDRI
460 470 480 490 500
YNMIASRPDW CISRQRIWGV PIVALICESC GEVWNDPSWM KKIAEFFAIH
510 520 530 540 550
PRGCDYWYEA KLEDIVPVGL KCPHCEGEQW KRESDILDVW FDSGSSFAAV
560 570 580 590 600
LEERPNLGFP ADLYLEGSDQ HRGWFHSSLL ISIGTRGVPP YHAVLTHGYV
610 620 630 640 650
VDGDGRKMSK SMGNVTSPQE IISKFGVEIL RLWVSSVDYR EDVRISNEIL
660 670 680 690 700
QRLVDAYRRI RNTCRYLLGN INDLTLDELV PVKEMESLDQ YILDVVATAY
710 720 730 740 750
TEIQKSYISY DFHTVFHKLH NLCTTDLSAF YLDILKDRLY TSGVRSHKRK
760 770 780 790 800
SAQTALFYIL HMLLRSMAPI LSFTAEEVYK YIPDTLKDDN VISVFMLPFF
810 820 830 840 850
ETSSFLLDDR VRSYWETLLL IRAEVNQAIE PMRKKGEIGH SLDTHITLYV
860 870 880 890 900
APELHTLLLE LNTDLCSLFI VSQLDIMPLS EASVDAAVSK IDGLAVAVNR
910 920 930
AQGNKCQRCW MYKELGSNHQ YPTLCPRCTE VVENMKI
Length:937
Mass (Da):107,965
Last modified:May 30, 2006 - v1
Checksum:i5CCC1C52FF4081CF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM180252 Genomic DNA. Translation: CAJ55105.1.
RefSeqiYP_595426.1. NC_008011.1.

Genome annotation databases

GeneIDi4059667.
KEGGilip:LI1051.
PATRICi22305659. VBILawInt40445_1144.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM180252 Genomic DNA. Translation: CAJ55105.1 .
RefSeqi YP_595426.1. NC_008011.1.

3D structure databases

ProteinModelPortali Q1MPH2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 363253.LI1051.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4059667.
KEGGi lip:LI1051.
PATRICi 22305659. VBILawInt40445_1144.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LINT363253:GH6E-1093-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Lawsonia intracellularis: the causative agent of proliferative enteropathy."
    Kaur K., Zhang Q., Beckler D., Munir S., Li L., Kinsley K., Herron L., Peterson A., May B., Singh S., Gebhart C., Kapur V.
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PHE/MN1-00.

Entry informationi

Entry nameiSYI_LAWIP
AccessioniPrimary (citable) accession number: Q1MPH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 30, 2006
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3