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Q1MPH2

- SYI_LAWIP

UniProt

Q1MPH2 - SYI_LAWIP

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Protein
Isoleucine--tRNA ligase
Gene
ileS, LI1051
Organism
Lawsonia intracellularis (strain PHE/MN1-00)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei566 – 5661Aminoacyl-adenylate By similarity
Binding sitei610 – 6101ATP By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity
Metal bindingi925 – 9251Zinc By similarity
Metal bindingi928 – 9281Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLINT363253:GH6E-1093-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:LI1051
OrganismiLawsonia intracellularis (strain PHE/MN1-00)
Taxonomic identifieri363253 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeLawsonia
ProteomesiUP000002430: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 937937Isoleucine--tRNA ligaseUniRule annotation
PRO_1000070889Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi363253.LI1051.

Structurei

3D structure databases

ProteinModelPortaliQ1MPH2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi607 – 6115"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1MPH2-1 [UniParc]FASTAAdd to Basket

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MTDYKETLNL PNTTFPMKAN LVQREPEIIL WWEENAVYEK MLEASGAKGI    50
FILHDGPPYA NGHIHLGTAL NKILKDIVIK SRNMQGYRSC YVPGWDCHGL 100
PIELKVEQEL GKKKQEMPLS LIRNRCREYA EKFLDIQREE FKRLGVFGSW 150
DHPYQTMDPI YESVITLELA RFVEKGSVIR SKKPIYWCYS CETALAEAEV 200
EYADHTSSAI FVRFPIHDKR LHTIFPQADL TTTSIVIWTT TPWTLPSNMA 250
IALNADFDYA LLQYKNEYII IASELVDICL KQFNWEDAKV IKVVQGKDLE 300
GMKARHPLYD QESMIVLGDH VTLEAGTGCV HTAPGHGPED YEVALRYNLD 350
VYSPLDDQGR YLDTVKFFAG LRVDQANPVV IQKLEEFHRI IQKNTIQHSY 400
PHCWRCKSPV IFRATTQWFI SMEKNNLREQ SLKAIKKNIE WIPSWGEDRI 450
YNMIASRPDW CISRQRIWGV PIVALICESC GEVWNDPSWM KKIAEFFAIH 500
PRGCDYWYEA KLEDIVPVGL KCPHCEGEQW KRESDILDVW FDSGSSFAAV 550
LEERPNLGFP ADLYLEGSDQ HRGWFHSSLL ISIGTRGVPP YHAVLTHGYV 600
VDGDGRKMSK SMGNVTSPQE IISKFGVEIL RLWVSSVDYR EDVRISNEIL 650
QRLVDAYRRI RNTCRYLLGN INDLTLDELV PVKEMESLDQ YILDVVATAY 700
TEIQKSYISY DFHTVFHKLH NLCTTDLSAF YLDILKDRLY TSGVRSHKRK 750
SAQTALFYIL HMLLRSMAPI LSFTAEEVYK YIPDTLKDDN VISVFMLPFF 800
ETSSFLLDDR VRSYWETLLL IRAEVNQAIE PMRKKGEIGH SLDTHITLYV 850
APELHTLLLE LNTDLCSLFI VSQLDIMPLS EASVDAAVSK IDGLAVAVNR 900
AQGNKCQRCW MYKELGSNHQ YPTLCPRCTE VVENMKI 937
Length:937
Mass (Da):107,965
Last modified:May 30, 2006 - v1
Checksum:i5CCC1C52FF4081CF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM180252 Genomic DNA. Translation: CAJ55105.1.
RefSeqiWP_011527134.1. NC_008011.1.
YP_595426.1. NC_008011.1.

Genome annotation databases

GeneIDi4059667.
KEGGilip:LI1051.
PATRICi22305659. VBILawInt40445_1144.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM180252 Genomic DNA. Translation: CAJ55105.1 .
RefSeqi WP_011527134.1. NC_008011.1.
YP_595426.1. NC_008011.1.

3D structure databases

ProteinModelPortali Q1MPH2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 363253.LI1051.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4059667.
KEGGi lip:LI1051.
PATRICi 22305659. VBILawInt40445_1144.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LINT363253:GH6E-1093-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Lawsonia intracellularis: the causative agent of proliferative enteropathy."
    Kaur K., Zhang Q., Beckler D., Munir S., Li L., Kinsley K., Herron L., Peterson A., May B., Singh S., Gebhart C., Kapur V.
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PHE/MN1-00.

Entry informationi

Entry nameiSYI_LAWIP
AccessioniPrimary (citable) accession number: Q1MPH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 30, 2006
Last modified: September 3, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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