Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q1MPC4 (MURE_LAWIP)

Last modified November 3, 2009. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase
Gene names
Name: murE
Ordered Locus Names: LI1099
OrganismLawsonia intracellularis (strain PHE/MN1-00) [Complete proteome] [HAMAP]
Taxonomic identifier363253 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Lawsonia

Hide | Top

Protein attributes

Sequence length482 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

Hide | Top

General annotation (Comments)

Function

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208

Subcellular location

Cytoplasm By similarity.

Post-translational modification

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.

Sequence similarities

Belongs to the murCDEF family. MurE subfamily.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 482482UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208
PRO_1000012369

Regions

Nucleotide binding105 – 1117ATP Potential
Region147 – 1482UDP-MurNAc-L-Ala-D-Glu binding By similarity
Region402 – 4054Meso-diaminopimelate binding By similarity
Motif402 – 4054Meso-diaminopimelate recognition motif HAMAP MF_00208

Sites

Binding site241UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1741UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1801UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1821UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site3781Meso-diaminopimelate By similarity
Binding site4531Meso-diaminopimelate; via carbonyl oxygen By similarity
Binding site4571Meso-diaminopimelate By similarity

Amino acid modifications

Modified residue2141N6-carboxylysine By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q1MPC4-1 [UniParc].

Last modified May 30, 2006. Version 1.
Checksum: 6B4DB5FF5EBE96C3

FASTA48253,605
        10         20         30         40         50         60 
MNRYPLKALE SELEKKHMLI CIDSRKVEKG CVFVALPGSS VDGGLFIPDA VSRGAAYIVC 

        70         80         90        100        110        120 
RHNEVEYCGS AIPIVVDDPR YTLGRLARIF YNTGNLSMPI IGVTGTNGKT TITYLLEYLF 

       130        140        150        160        170        180 
RAKGNRTGVI GTIAYRWPGF SKEAPLTTPQ CLDLHAMLAQ MQVDKTEIVF MEVSSHALDQ 

       190        200        210        220        230        240 
RRIAGISFKG VIFTNLTQDH LDYHKDMKEY FHAKARLFFE YPSKNKIMVA SMDNQWGRKL 

       250        260        270        280        290        300 
AKLVPEIIGF GFKNKPTQVS NYLFGKILSS SRAGLHLQMS FKDKVWELCT PLVGVHNAEN 

       310        320        330        340        350        360 
LLAVQAISLQ LGLAPEDFCC FEKFTGVPGR LERIVNKKQL DIFVDYAHTP DALINVLSAL 

       370        380        390        400        410        420 
RDVGFKRIIT VFGCGGNRDK AKRPLMGKAV AKLSDVAVLT SDNPRNEDPE LIMADVLPGL 

       430        440        450        460        470        480 
KKAKQIITEP DREKAIRQAI ELVSPGDALL VAGKGHECTQ QIGFMKYPFS DQSVIRKILG 


CD

« Hide

Hide | Top

References

[1]"The complete genome sequence of Lawsonia intracellularis: the causative agent of proliferative enteropathy."
Kaur K., Zhang Q., Beckler D., Munir S., Li L., Kinsley K., Herron L., Peterson A., May B., Singh S., Gebhart C., Kapur V.
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Hide | Top

Cross-references

Sequence databases

AM180252 Genomic DNA. Translation: CAJ55153.1.
RefSeqYP_595474.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ1MPC4.

Genome annotation databases

GeneID4060077.
GenomeReviewsGene locus LI1099 in contig AM180252_GR.
KEGGlip:LI1099.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ1MPC4.
OMAHTPDGIE.

Family and domain databases

HAMAPMF_00208.
[Tree]
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
PfamPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
TIGRFAMsTIGR01085. murE. 1 hit.
ProtoNetSearch...

Hide | Top

Entry information

Entry nameMURE_LAWIP
AccessionPrimary (citable) accession number: Q1MPC4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 30, 2006
Last modified: November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents