Reviewed, UniProtKB/Swiss-Prot Q1MNB0 (MASZ_RHIL3)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	RL0054

Organism

Rhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP]

Taxonomic identifier

216596 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 723	723	Malate synthase G HAMAP MF_00641		PRO_1000056922
Sites
	Active site	338	1	Proton acceptor By similarity
	Active site	629	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1MNB0-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 5761ACD754F724D7	FASTA	723	79,684
	10 20 30 40 50 60 MSRVDKNGLA IETVLHDFLV EEVLPGLAVD ADKFFADFSA IVHDLAPKNC ALLAKRDELQ 70 80 90 100 110 120 VKIDDWYRRH GAPADMDEYQ SFLREIGYLL PEGSDFQVST QNVDPEIASI AGPQLVVPVM 130 140 150 160 170 180 NARYALNAAN ARWGSLYDAL YGTDAIPESD GAEKGKSYNP KRGEKVIAWV RDFLDTSAPL 190 200 210 220 230 240 QDCRWKDVGS FAVKDGALVV RSIDGEQAML TDGKHFAGYR GDAAAPTHIL LKNNGIHIEI 250 260 270 280 290 300 VIDAATTIGK ADPAHISDVW LESAITTIMD CEDSIAAVDA EDKVVVYRNW LGLMKGDLQE 310 320 330 340 350 360 EVAKGGTSFI RTLNPDLQYA GPDGAAFEVH RRSLMLVRNV GHLMISPAIL DRDGNEVPEG 370 380 390 400 410 420 IMDAAITGLI ALYDIGPSGR RKNSRTGSMY VVKPKMHGPE EVAFAVEIFS RVEDALGLPR 430 440 450 460 470 480 NTIKMGIMDE ERRTTVNLKE CIRAARERVV FINTGFLDRT GDEIHTSMEA GPMIRKGDMR 490 500 510 520 530 540 QAAWISAYEN WNVDIGLECG LAGHAQIGKG MWAMPDLMAA MLEQKIAHPK AGANTAWVPS 550 560 570 580 590 600 PTAATLHATH YHRVNVARVQ QGLKDRARAK LSDILSVPVA VRPNWTPEEI QRELDNNAQG 610 620 630 640 650 660 ILGYVVRWVD QGVGCSKVPD INNVGLMEDR ATLRISAQHM ANWLHHKVVT EAQIIETMRR 670 680 690 700 710 720 MAAVVDRQNA SDPAYQPMAG NFDDSIAFQA ALDLVLKGRE QPNGYTEPVL HRRRLELKAK HTA

References

[1]

"The genome of Rhizobium leguminosarum has recognizable core and accessory components." Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F., Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H., East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I., Chillingworth T., Clarke K. , Cronin A., Davis P., Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J. Genome Biol. 7:RESEARCH34.1-RESEARCH34.20(2006) [PubMed: 16640791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM236080 Genomic DNA. Translation: CAK05542.1.
RefSeq	YP_765658.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1MNB0.
Genome annotation databases
GeneID	4400340.
GenomeReviews	Gene locus RL0054 in contig AM236080_GR.
KEGG	rle:RL0054.
NMPDR	fig|216596.1.peg.66.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1MNB0.
OMA	PKMHGPD.
Family and domain databases
HAMAP	MF_00641. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_RHIL3

Accession

Primary (citable) accession number: Q1MNB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 30, 2006
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)