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Q1MMC3 (TRMB_RHIL3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:RL0388
OrganismRhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP]
Taxonomic identifier216596 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Protein attributes

Sequence length233 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 233233tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288210

Regions

Region212 – 2154Substrate binding By similarity

Sites

Active site1381 By similarity
Binding site641S-adenosyl-L-methionine By similarity
Binding site891S-adenosyl-L-methionine By similarity
Binding site1161S-adenosyl-L-methionine By similarity
Binding site1381S-adenosyl-L-methionine By similarity
Binding site1421Substrate By similarity
Binding site1741Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1MMC3 [UniParc].

Last modified May 30, 2006. Version 1.
Checksum: 8B25F6B0E0355941

FASTA23326,637
        10         20         30         40         50         60 
MTDMERRGRA TEAFFGRRKG KALREQQAET LNSLLPAFLI DLSAAPPEPL TSLFPVPVER 

        70         80         90        100        110        120 
LRLEIGFGGG EHLIHRALET PSTGFIGVEP FVNSMQKLLS RIGETGASNI RVYNDDATQL 

       130        140        150        160        170        180 
LDWLPDGALD QIDLLYPDPW PKRKHWKRRF VSKTNLDRFH RVLKPGGLFC FASDIDTYVN 

       190        200        210        220        230 
WTLIKCRDHG GFEWIADNAA DWLTPYEGWP STRYEAKARR EGRSSAYLTF RKV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM236080 Genomic DNA. Translation: CAK05879.1.
RefSeqYP_765995.1. NC_008380.1.

3D structure databases

ProteinModelPortalQ1MMC3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING216596.RL0388.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAK05879; CAK05879; RL0388.
GeneID4399088.
KEGGrle:RL0388.
PATRIC23137163. VBIRhiLeg32091_1533.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMAIGCEPFV.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycRLEG216596:GKE5-403-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_RHIL3
AccessionPrimary (citable) accession number: Q1MMC3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 30, 2006
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways