Q1MKV3 (LPXK_RHIL3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	RL0904

Organism

Rhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP]

Taxonomic identifier

216596 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_0000291232
Regions
	Nucleotide binding	54 – 61	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1MKV3 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 818F77823D8BBD38	FASTA	357	38,370
	10 20 30 40 50 60 MISEAPPFWW RKADWRAWLL LPLSFLYGRI AGHRMAHARR ASVPVPVICV GNFTVGGAGK 70 80 90 100 110 120 TPTAVTIARA AKAKGLKPGF LSRGYGGSLD VTTVVDPDHH RAIAVGDEPL LLAQEALTVI 130 140 150 160 170 180 SRRRVEGAAR LVAEGADLII MDDGFQSARL AIDYALLVID STRGLGNGHI VPGGPVRAPI 190 200 210 220 230 240 GQQLRSATAL LKVGGGNAAD RIVRMAARAA KPYFTASLRV RGDNTLAGTK VLAFAGIADP 250 260 270 280 290 300 AKFFRTVESR GAEIVVAKSF GDHEHLTEEE IDDILTTAER EGLLIVTTSK DFVRLSGHHG 310 320 330 340 350 KAARLVEKSR VIEVDMVFED HLAPSLIIDR AIVACRERRL REMKAGVKAI PAKAEPL

References

[1]

"The genome of Rhizobium leguminosarum has recognizable core and accessory components." Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F., Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H., East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I., Chillingworth T., Clarke K. , Cronin A., Davis P., Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J. Genome Biol. 7:R34.1-R34.20(2006) [PubMed: 16640791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 3841.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM236080 Genomic DNA. Translation: CAK06401.1.
RefSeq	YP_766516.1. NC_008380.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1MKV3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4403780.
GenomeReviews	Gene locus RL0904 in contig AM236080_GR.
KEGG	rle:RL0904.
NMPDR	fig|216596.1.peg.951.
PATRIC	23138239. VBIRhiLeg32091_2064.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1663.
HOGENOM	HBG663914.
OMA	VILMDDG.
PhylomeDB	Q1MKV3.
ProtClustDB	PRK00652.
Enzyme and pathway databases
BioCyc	RLEG216596:RL0904-MONOMER.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_RHIL3

Accession

Primary (citable) accession number: Q1MKV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	May 30, 2006
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program