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Protein

Lipopolysaccharide core galacturonosyltransferase RgtA

Gene

rgtA

Organism
Rhizobium leguminosarum bv. viciae (strain 3841)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the modification of the lipopolysaccharide (LPS) inner core. Catalyzes the transfer of a galacturonic acid (GalA) residue to the 4-position of the outer Kdo (3-deoxy-D-manno-octulosonic acid) residue of the LPS inner core, using dodecaprenyl phosphate-GalA as the donor substrate. GalA addition by RgtA is required for RgtB activity.3 Publications

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.1 Publication1 Publication
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

  • galactosyltransferase activity Source: CACAO
  • transferase activity, transferring hexosyl groups Source: UniProtKB

GO - Biological processi

  • lipopolysaccharide biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciRLEG216596:GKE5-1500-MONOMER.
UniPathwayiUPA00958.

Protein family/group databases

CAZyiGT83. Glycosyltransferase Family 83.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide core galacturonosyltransferase RgtA1 Publication (EC:2.4.1.-1 Publication)
Short name:
LPS core GalA transferase RgtA1 Publication
Alternative name(s):
Dodecaprenyl phosphate-beta-galacturonate:lipopolysaccharide core alpha-galacturonosyltransferase RgtA1 Publication
Short name:
Dodecaprenyl-P-GalA:LPS core galacturonosyltransferase RgtA2 Publications
Galacturonic acid transferase RgtA1 Publication
Short name:
GalAT RgtA1 Publication
Gene namesi
Name:rgtA1 PublicationImported
Ordered Locus Names:RL1469Imported
OrganismiRhizobium leguminosarum bv. viciae (strain 3841)
Taxonomic identifieri216596 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000006575 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei11 – 3121HelicalSequence analysisAdd
BLAST
Transmembranei74 – 9421HelicalSequence analysisAdd
BLAST
Transmembranei103 – 12321HelicalSequence analysisAdd
BLAST
Transmembranei125 – 14521HelicalSequence analysisAdd
BLAST
Transmembranei165 – 18521HelicalSequence analysisAdd
BLAST
Transmembranei199 – 21921HelicalSequence analysisAdd
BLAST
Transmembranei248 – 26821HelicalSequence analysisAdd
BLAST
Transmembranei291 – 31121HelicalSequence analysisAdd
BLAST
Transmembranei316 – 33621HelicalSequence analysisAdd
BLAST
Transmembranei351 – 37121HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene produce a normal lipid A structure with GalA at the 4'-position but a modified LPS core structure as this gene deletion results in the complete absence of GalA on both the 4- and 5-positions of the outer Kdo residue and incomplete addition of GalA to the Man residue. The mutant cells are also more susceptible to deoxycholic acid when compared with the parent strain, and more resistant to the polycationic antimicrobial peptide PmxB.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Lipopolysaccharide core galacturonosyltransferase RgtAPRO_0000436509Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi216596.RL1469.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 83 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108VDT. Bacteria.
COG1807. LUCA.
HOGENOMiHOG000295384.
OMAiTTVRERW.
OrthoDBiPOG091H2BMQ.

Sequencei

Sequence statusi: Complete.

Q1MJ96-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLERATRTIK TAGLLLAAYF VLNIVLRIVL PHSLELDEAE QSFFSQYLLA
60 70 80 90 100
GYGPQPPFYN WMQYAVVSVT GISIGALIVP KNILLFLSYL FYGLAGRRVL
110 120 130 140 150
KDEALAAVGM LALITLPQVS YMAQQDLTHT TALLFASSLF LYGFFRTLDR
160 170 180 190 200
PDMASYLLLG LATGIGLISK YNFALMPVVA LIAILPDAEW RRRALDWRML
210 220 230 240 250
AAITVALVIV LPHAVWLQGN LAFASSDTLV KMAAGSEPAG AVRIGKGLLA
260 270 280 290 300
FLVAIIAFAA LPVVIFAATF RRDFVRALSA GNRWTGMMER MMLASLAGIA
310 320 330 340 350
LIVLFTGSTT VRERWLDPFL LVLPIYFLAK MQAAGLDLSA GLRRFRPVLP
360 370 380 390 400
VLMACVLIAL GFRVVGAGLI GTYSRPNVPM AGFAREMTRQ AEPALVIASD
410 420 430 440 450
TYIGGNMRLQ FPDVPVVIPD FPAPGIPAYA EAKGPVLIVW RGKKTATAAD
460 470 480 490
AVMPERFSSA LTAAGIALQE IGSLSLPYYF GRQGDNFALG YAWVRPETR
Length:499
Mass (Da):54,261
Last modified:May 30, 2006 - v1
Checksum:i818A47F74B248CE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ298016 Genomic DNA. Translation: ABC02169.1.
AM236080 Genomic DNA. Translation: CAK06964.1.
RefSeqiWP_011651167.1. NC_008380.1.

Genome annotation databases

EnsemblBacteriaiCAK06964; CAK06964; RL1469.
KEGGirle:RL1469.
PATRICi23139445. VBIRhiLeg32091_2665.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ298016 Genomic DNA. Translation: ABC02169.1.
AM236080 Genomic DNA. Translation: CAK06964.1.
RefSeqiWP_011651167.1. NC_008380.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216596.RL1469.

Protein family/group databases

CAZyiGT83. Glycosyltransferase Family 83.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAK06964; CAK06964; RL1469.
KEGGirle:RL1469.
PATRICi23139445. VBIRhiLeg32091_2665.

Phylogenomic databases

eggNOGiENOG4108VDT. Bacteria.
COG1807. LUCA.
HOGENOMiHOG000295384.
OMAiTTVRERW.
OrthoDBiPOG091H2BMQ.

Enzyme and pathway databases

UniPathwayiUPA00958.
BioCyciRLEG216596:GKE5-1500-MONOMER.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiRGTA_RHIL3
AccessioniPrimary (citable) accession number: Q1MJ96
Secondary accession number(s): Q20DQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 6, 2016
Last sequence update: May 30, 2006
Last modified: September 7, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.