Q1MIW9 (PUR5_RHIL3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	RL1596

Organism

Rhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP]

Taxonomic identifier

216596 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium › Rhizobium leguminosarum › Rhizobium leguminosarum bv. viciae

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00741.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741		PRO_0000258392

Sequences

	Sequence		Length	Mass (Da)
	Q1MIW9 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: CF0D5FF7BCB12FF3	FASTA	357	36,743
	10 20 30 40 50 60 MSQSGKNGLT YSDAGVDIDA GNLLVEKIKP AVRSTRRPGA DGEIGGFGGL FDLKAAGFTD 70 80 90 100 110 120 PVLVAANDGV GTKLKIAIDA DYHDTVGIDL VAMCVNDLVV QGAEPLFFLD YFATGKLDPD 130 140 150 160 170 180 QGAAIVGGIA AGCREAGCAL IGGETAEMPG MYSSGDYDLA GFAVGAAERG KLLPSGDIAE 190 200 210 220 230 240 GDVILGLASS GVHSNGFSLV RKIVELSGLD WDSPAPFTED KKLGEALLEP TRIYVKPLLK 250 260 270 280 290 300 AIRETGAIKA LAHITGGGFP ENIPRVLPKH LAAEIDLATV KVPPVFSWLA KTGGVEAKEM 310 320 330 340 350 LRTFNCGVGM IAVVAGENVA TVSAALEAEG ETVITLGRMI AREEGAAGTV YKGTLAI

References

[1]

"The genome of Rhizobium leguminosarum has recognizable core and accessory components." Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F., Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H., East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I., Chillingworth T., Clarke K. , Cronin A., Davis P., Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J. Genome Biol. 7:R34.1-R34.20(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 3841.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM236080 Genomic DNA. Translation: CAK07091.1.
RefSeq	YP_767200.1. NC_008380.1.
3D structure databases
ProteinModelPortal	Q1MIW9.
SMR	Q1MIW9. Positions 8-344.
ModBase	Search...
Protein-protein interaction databases
STRING	216596.RL1596.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAK07091; CAK07091; RL1596.
GeneID	4399233.
KEGG	rle:RL1596.
PATRIC	23139727. VBIRhiLeg32091_2805.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0150.
HOGENOM	HOG000229090.
KO	K01933.
OMA	PRVLPKH.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	RLEG216596:GKE5-1628-MONOMER.
UniPathway	UPA00074; UER00129.
Family and domain databases
Gene3D	3.90.650.10. 1 hit.
HAMAP	MF_00741_B. AIRS_B.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_RHIL3

Accession

Primary (citable) accession number: Q1MIW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	May 30, 2006
Last modified:	May 29, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program