Reviewed, UniProtKB/Swiss-Prot Q1MI52 (HGD_RHIL3)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisicase
    Homogentisate oxygenase
    Homogentisic acid oxidase

Gene names

Name:	hmgA
Ordered Locus Names:	RL1864

Organism

Rhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP]

Taxonomic identifier

216596 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Homogentisate + O(2) = 4-maleylacetoacetate.
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 4/6.
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	Homogentisate 1,2-dioxygenase		PRO_1000019539
Sites
	Metal binding	349	1	Iron By similarity
	Metal binding	355	1	Iron By similarity
	Metal binding	385	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1MI52-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: F38F46C99C31976C	FASTA	453	50,888
	10 20 30 40 50 60 MDQTSIQTSD GEAATANTLK YMPGFGNDFE TESLPGALPQ GQNSPQKCNY GLYAEQLSGS 70 80 90 100 110 120 PFTAPRGTNE RSWLYRIRPS VRHTRRFSNA SYPLWKTAPC LDEHSLPLGQ LRWDPIPAPA 130 140 150 160 170 180 ERLTFLEGVR TITTAGDAAT QVGMSAHAYV FNEDMVDDYF FNADGELLIV PQLGAIRVFT 190 200 210 220 230 240 EMGIMDVEPL EICLIPRGMM FKILKSGEQT VWRGYICENY GAKFTLPDRG PIGANCLANP 250 260 270 280 290 300 RDFKTPVAAF EDKETPCRVH VKWCGKFYVT EIGHSPLDVV AWHGNYAPFK YDLRTFSPVG 310 320 330 340 350 360 AIRFDHPDPS IFSVLTAPSE DAGTANVDFV IFPPRWLVAE HTFRPPWYHR NIMSEFMGLI 370 380 390 400 410 420 HGQYDAKEEG FVPGGISLHN MMLPHGPDAL AFEKASNTEL KPVKLDHTMA FMFETRYAQQ 430 440 450 LTKYAAELET LQDNYLECWD GLERKFDGTP GIK

References

[1]

"The genome of Rhizobium leguminosarum has recognizable core and accessory components." Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F., Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H., East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I., Chillingworth T., Clarke K. , Cronin A., Davis P., Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J. Genome Biol. 7:RESEARCH34.1-RESEARCH34.20(2006) [PubMed: 16640791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM236080 Genomic DNA. Translation: CAK07358.1.
RefSeq	YP_767467.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4402170.
GenomeReviews	Gene locus RL1864 in contig AM236080_GR.
KEGG	rle:RL1864.
NMPDR	fig|216596.1.peg.1951.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1MI52.
Family and domain databases
HAMAP	MF_00334. [Tree]
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_RHIL3

Accession

Primary (citable) accession number: Q1MI52

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 30, 2006
Last modified:	November 4, 2008
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)