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Q1MI52 (HGD_RHIL3) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homogentisate 1,2-dioxygenase

Short name=HGDO
EC=1.13.11.5
Alternative name(s):
Homogentisate oxygenase
Homogentisic acid oxidase
Homogentisicase
Gene names
Name:hmgA
Ordered Locus Names:RL1864
OrganismRhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP]
Taxonomic identifier216596 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Protein attributes

Sequence length453 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the catabolism of homogentisate (2,5-dihydroxyphenylacetate or 2,5-OH-PhAc), a central intermediate in the degradation of phenylalanine and tyrosine. Catalyzes the oxidative ring cleavage of the aromatic ring of homogentisate to yield maleylacetoacetate By similarity. HAMAP-Rule MF_00334

Catalytic activity

Homogentisate + O2 = 4-maleylacetoacetate. HAMAP-Rule MF_00334

Cofactor

Iron By similarity. HAMAP-Rule MF_00334

Pathway

Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP-Rule MF_00334

Subunit structure

Hexamer; dimer of trimers By similarity. HAMAP-Rule MF_00334

Sequence similarities

Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
   Biological processPhenylalanine catabolism
Tyrosine catabolism
   LigandIron
Metal-binding
   Molecular functionDioxygenase
Oxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processL-phenylalanine catabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tyrosine catabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionhomogentisate 1,2-dioxygenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

iron ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 453453Homogentisate 1,2-dioxygenase HAMAP-Rule MF_00334
PRO_1000019539

Sites

Active site3061Proton acceptor By similarity
Metal binding3491Iron By similarity
Metal binding3551Iron By similarity
Metal binding3851Iron By similarity
Binding site3641homogentisate By similarity
Binding site3851homogentisate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1MI52 [UniParc].

Last modified May 30, 2006. Version 1.
Checksum: F38F46C99C31976C

FASTA45350,888
        10         20         30         40         50         60 
MDQTSIQTSD GEAATANTLK YMPGFGNDFE TESLPGALPQ GQNSPQKCNY GLYAEQLSGS 

        70         80         90        100        110        120 
PFTAPRGTNE RSWLYRIRPS VRHTRRFSNA SYPLWKTAPC LDEHSLPLGQ LRWDPIPAPA 

       130        140        150        160        170        180 
ERLTFLEGVR TITTAGDAAT QVGMSAHAYV FNEDMVDDYF FNADGELLIV PQLGAIRVFT 

       190        200        210        220        230        240 
EMGIMDVEPL EICLIPRGMM FKILKSGEQT VWRGYICENY GAKFTLPDRG PIGANCLANP 

       250        260        270        280        290        300 
RDFKTPVAAF EDKETPCRVH VKWCGKFYVT EIGHSPLDVV AWHGNYAPFK YDLRTFSPVG 

       310        320        330        340        350        360 
AIRFDHPDPS IFSVLTAPSE DAGTANVDFV IFPPRWLVAE HTFRPPWYHR NIMSEFMGLI 

       370        380        390        400        410        420 
HGQYDAKEEG FVPGGISLHN MMLPHGPDAL AFEKASNTEL KPVKLDHTMA FMFETRYAQQ 

       430        440        450 
LTKYAAELET LQDNYLECWD GLERKFDGTP GIK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM236080 Genomic DNA. Translation: CAK07358.1.
RefSeqYP_767467.1. NC_008380.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING216596.RL1864.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAK07358; CAK07358; RL1864.
GeneID4402170.
KEGGrle:RL1864.
PATRIC23140329. VBIRhiLeg32091_3098.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3508.
HOGENOMHOG000139824.
KOK00451.
OMATTETHKL.
OrthoDBEOG6D5FZK.

Enzyme and pathway databases

BioCycRETL1328306-WGS:GSTH-1787-MONOMER.
RLEG216596:GKE5-1903-MONOMER.
UniPathwayUPA00139; UER00339.

Family and domain databases

Gene3D2.60.120.10. 2 hits.
HAMAPMF_00334. Homogentis_dioxygen.
InterProIPR005708. Homogentis_dOase.
IPR022950. Homogentis_dOase_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERPTHR11056. PTHR11056. 1 hit.
PfamPF04209. HgmA. 1 hit.
[Graphical view]
SUPFAMSSF51182. SSF51182. 1 hit.
TIGRFAMsTIGR01015. hmgA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHGD_RHIL3
AccessionPrimary (citable) accession number: Q1MI52
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 30, 2006
Last modified: July 9, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways