Q1MH44 (LPXA_RHIL3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
Short name=UDP-N-acetylglucosamine acyltransferase
EC=2.3.1.129

Gene names

Name:	lpxA
Ordered Locus Names:	RL2231

Organism

Rhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP]

Taxonomic identifier

216596 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00387
Catalytic activity	(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetylglucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetylglucosamine. HAMAP MF_00387
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 1/6. HAMAP MF_00387
Subunit structure	Homotrimer By similarity. HAMAP MF_00387
Subcellular location	Cytoplasm By similarity HAMAP MF_00387.
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxA subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase HAMAP MF_00387		PRO_1000013175

Sequences

	Sequence		Length	Mass (Da)
	Q1MH44 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 3F2E8FA0131A1B97	FASTA	272	28,514
	10 20 30 40 50 60 MSTIAESARI HPMAVVEDGA TIGEGVKIGP FCHVGPHVVL HANVELLSHA IVTGRTVIGK 70 80 90 100 110 120 GTRIFPMAVV GGDPQSVHHG GEETTLTVGA NCTIREGVTM NTGTADFGGQ TIVGDNNLFL 130 140 150 160 170 180 ANSHVAHDCR VGNHVIMSNN VMLAGHVVIE DRVILGGGSA VHQFTRVGRQ AFVGGLSAVS 190 200 210 220 230 240 YDVIPYGMLN GNPGLLSGLN VVGMTRAGVD RAVIHRVRRA YKSIFEGPGS VRENAAAIRE 250 260 270 EYADCEQAVH ILDFIAADSD RALSSPTRGQ KG

References

[1]

"The genome of Rhizobium leguminosarum has recognizable core and accessory components." Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F., Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H., East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I., Chillingworth T., Clarke K. , Cronin A., Davis P., Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J. Genome Biol. 7:R34.1-R34.20(2006) [PubMed: 16640791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 3841.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM236080 Genomic DNA. Translation: CAK07723.1.
RefSeq	YP_767825.1. NC_008380.1.
3D structure databases
ProteinModelPortal	Q1MH44.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1MH44.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4403775.
GenomeReviews	Gene locus RL2231 in contig AM236080_GR.
KEGG	rle:RL2231.
NMPDR	fig|216596.1.peg.2312.
PATRIC	23141063. VBIRhiLeg32091_3456.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1043.
HOGENOM	HBG659295.
OMA	REFCTFN.
PhylomeDB	Q1MH44.
ProtClustDB	PRK05289.
Enzyme and pathway databases
BioCyc	RLEG216596:RL2231-MONOMER.
Family and domain databases
HAMAP	MF_00387. LpxA. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR010137. Lipid_A_lpxA. IPR011004. Trimer_LpxA-like. [Graphical view]
KO	K00677.
Pfam	PF00132. Hexapep. 3 hits. [Graphical view]
PIRSF	PIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01852. Lipid_A_lpxA. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXA_RHIL3

Accession

Primary (citable) accession number: Q1MH44

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 30, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program