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Protein

Lipoyl synthase

Gene

lipA

Organism
Rhizobium leguminosarum bv. viciae (strain 3841)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Catalytic activityi

Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.

Cofactori

[4Fe-4S] clusterNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61Iron-sulfur 1 (4Fe-4S)1
Metal bindingi66Iron-sulfur 1 (4Fe-4S)1
Metal bindingi72Iron-sulfur 1 (4Fe-4S)1
Metal bindingi87Iron-sulfur 2 (4Fe-4S-S-AdoMet)1
Metal bindingi91Iron-sulfur 2 (4Fe-4S-S-AdoMet)1
Metal bindingi94Iron-sulfur 2 (4Fe-4S-S-AdoMet)1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:RL2249
OrganismiRhizobium leguminosarum bv. viciae (strain 3841)
Taxonomic identifieri216596 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000006575 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000122601 – 323Lipoyl synthaseAdd BLAST323

Interactioni

Protein-protein interaction databases

STRINGi216596.RL2249.

Structurei

3D structure databases

ProteinModelPortaliQ1MH26.
SMRiQ1MH26.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Phylogenomic databases

eggNOGiENOG4105C0G. Bacteria.
COG0320. LUCA.
HOGENOMiHOG000235997.
KOiK03644.
OMAiPYCDIDF.
OrthoDBiPOG091H069D.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_00206. Lipoyl_synth. 1 hit.
InterProiView protein in InterPro
IPR006638. Elp3/MiaB/NifB.
IPR031691. LIAS_N.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
PfamiView protein in Pfam
PF16881. LIAS_N. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SFLDiSFLDG01058. lipoyl_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1MH26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTILDTINP DAKRVRHPEK AHRPDTEVMR KPDWIRVKAP TSKGYAETRA
60 70 80 90 100
IVKEHKLVTV CEEAGCPNIG ECWDKKHATF MIMGEICTRA CAFCNVATGK
110 120 130 140 150
PNALDMAEPE NVAKAVKEMG LSHVVITSVD RDDLEDGGAE HFEKVIWAIR
160 170 180 190 200
AASPMTTIEI LTPDFLKKPG ALERVVAAKP DVFNHNMETV PGNYLTVRPG
210 220 230 240 250
ARYFHSVRLL QRVKELDPTM FTKSGIMVGL GEERNEVLQL MDDLRTADVD
260 270 280 290 300
FLTIGQYLQP TRKHHKVESF VTPDEFKSYE TVAYSKGFLM VASSPLTRSS
310 320
HHAGDDFARL RAAREKKLLM AAE
Length:323
Mass (Da):36,188
Last modified:May 30, 2006 - v1
Checksum:iB173D74405BD0073
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM236080 Genomic DNA. Translation: CAK07741.1.
RefSeqiWP_003559117.1. NC_008380.1.

Genome annotation databases

EnsemblBacteriaiCAK07741; CAK07741; RL2249.
KEGGirle:RL2249.

Similar proteinsi

Entry informationi

Entry nameiLIPA_RHIL3
AccessioniPrimary (citable) accession number: Q1MH26
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 30, 2006
Last modified: October 25, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families