Reviewed,
UniProtKB/Swiss-Prot Q1MH21 (ISPDF_RHIL3)
Last modified
November 3, 2009.
Version 23.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (2) |
 Third-party data |
 Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Bifunctional enzyme ispD/ispF Including the following 2 domains: 1- Recommended name: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase EC=2.7.7.60 Alternative name(s): 4-diphosphocytidyl-2C-methyl-D-erythritol synthase MEP cytidylyltransferase Short name=MCT 2- Recommended name: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase Short name=MECPS Short name=MECDP-synthase EC=4.6.1.12 | ||||
| Gene names |
| ||||
| Organism | Rhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 216596 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium |
Protein attributes
| Sequence length | 405 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (ispD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (ispF) By similarity. |
| Catalytic activity | CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520 |
| Cofactor | Divalent metal cations By similarity. |
| Pathway | Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520 |
| Sequence similarities | In the N-terminal section; belongs to the ispD family. In the C-terminal section; belongs to the ispF family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Isoprene biosynthesis |
| Ligand | Metal-binding |
| Molecular function | Lyase Nucleotidyltransferase Transferase |
| Technical term | Complete proteome Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP |
| Molecular function | 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: HAMAP 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activityInferred from electronic annotation. Source: HAMAP metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 405 | 405 | Bifunctional enzyme ispD/ispF HAMAP MF_01520 | PRO_0000296751 | |||||
Regions | |||||||||
| Region | 1 – 246 | 246 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520 | ||||||
| Region | 247 – 405 | 159 | 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520 | ||||||
Sites | |||||||||
| Metal binding | 253 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 255 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 287 | 1 | Divalent metal cation By similarity | ||||||
| Site | 24 | 1 | Transition state stabilizer By similarity | ||||||
| Site | 33 | 1 | Transition state stabilizer By similarity | ||||||
| Site | 167 | 1 | Positions MEP for the nucleophilic attack By similarity | ||||||
| Site | 224 | 1 | Positions MEP for the nucleophilic attack By similarity | ||||||
| Site | 279 | 1 | Transition state stabilizer By similarity | ||||||
| Site | 378 | 1 | Transition state stabilizer By similarity | ||||||
Sequences
| ||||||||||||||||||
References
| [1] | "The genome of Rhizobium leguminosarum has recognizable core and accessory components." Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F., Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H., East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I., Chillingworth T., Clarke K.  Parkhill J.Genome Biol. 7:RESEARCH34.1-RESEARCH34.20(2006) [PubMed: 16640791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| AM236080 Genomic DNA. Translation: CAK07746.1. | |
| RefSeq | YP_767848.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q1MH21. |
Genome annotation databases | |
| GeneID | 4402763. |
| GenomeReviews | Gene locus RL2254 in contig AM236080_GR. |
| KEGG | rle:RL2254. |
| NMPDR | fig|216596.1.peg.2335. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | Q1MH21. |
| OMA | IVLIHDA. |
Family and domain databases | |
| HAMAP | MF_01520. [Tree] |
| InterPro | IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. IPR003526. MECDP_synthase_core. IPR020555. MECDP_synthase_CS. [Graphical view] |
| Pfam | PF01128. IspD. 1 hit. PF02542. YgbB. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00453. ispD. 1 hit. TIGR00151. ispF. 1 hit. |
| PROSITE | PS01295. ISPD. 1 hit. PS01350. ISPF. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ISPDF_RHIL3 | ||||||||
| Accession | Primary (citable) accession number: Q1MH21 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
