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Q1MFI6 (SYL1_RHIL3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase 1

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase 1
Short name=LeuRS 1
Gene names
Name:leuS1
Ordered Locus Names:RL2798
OrganismRhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP]
Taxonomic identifier216596 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Protein attributes

Sequence length838 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 838838Leucine--tRNA ligase 1 HAMAP-Rule MF_00049
PRO_0000334802

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif608 – 6125"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6111ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1MFI6 [UniParc].

Last modified May 30, 2006. Version 1.
Checksum: 7C2AC9CEACE16F9F

FASTA83894,069
        10         20         30         40         50         60 
MPYDPTKIEP KWQAYWAGHH IFRVEIDPAR PKFYALDMFP YPSGAGLHVG HPLGYTATDI 

        70         80         90        100        110        120 
MCRYKRMRGF NVLHPMGWDS FGLPAERHAM RTGVHPDITT KRNIETFRGQ VQRLGFSYDW 

       130        140        150        160        170        180 
SREFATTDPA YVRWTQWIFL KLFENGLAYQ AEVAVNWCPA QNAVLADEEV KDGRYVETGD 

       190        200        210        220        230        240 
PVIRRRMRQW MLRITAYADR LLQGLDGLDW PENLKAMQRN WIGRSEGAEI RFPLEHGKGV 

       250        260        270        280        290        300 
ITVFTTRPET LFGASYILLA PEHPAVAAIV EPEMSEAVAA YIAEAEGLEE TVRADAGREK 

       310        320        330        340        350        360 
TGVFTGAYAI NPANGARLPV WVADYVLAGY GTGALMAVPA HDARDYAFAH SHELPIIRVI 

       370        380        390        400        410        420 
DSEADIEKGA YEGEGAMVNS GFLSGLGSPE ARSAIVAWLQ ANGTGWPKVM YRLRDWLFSR 

       430        440        450        460        470        480 
QRYWGEPIPV LHLADGSVMP LPEECLPLLP PELDDYAPTP DGEPPLARAQ AWVETIVPGT 

       490        500        510        520        530        540 
DIPARRETNT MPQWAGSCWY YLRFLDPENT SEPVGREAER YWMPVDLYVG GAEHAVLHLL 

       550        560        570        580        590        600 
YARFWHKVLY DIGVVSTEEP FQRLFNQGMI LAHSYRDAAG RYYAPSSVVE EEGRWFAGSV 

       610        620        630        640        650        660 
EVQRAVEKMS KSQLNVVNPD DVVHQFGADA LRLYEMFMGP LDAAKPWQTA GVIGVRRFLE 

       670        680        690        700        710        720 
RAWRIVCDES DGLSPVVLEA APSPQLLRLR HQTVKTVTAD IEAIRFNTAV SRLMELANAL 

       730        740        750        760        770        780 
TAEAARPREV VETFVLLLAP FAPHIAEELW SKLGHGETLT WVSWPTFDPA LIEMETREYV 

       790        800        810        820        830 
VQINGKVRHR FEAAADLGEA LLAAARSEPS VMALLDGKTV VKEVLVPGRL VNFVVEDL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM236080 Genomic DNA. Translation: CAK08288.1.
RefSeqYP_768383.1. NC_008380.1.

3D structure databases

ProteinModelPortalQ1MFI6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING216596.RL2798.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAK08288; CAK08288; RL2798.
GeneID4400999.
KEGGrle:RL2798.
PATRIC23142269. VBIRhiLeg32091_4056.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAWVETIVP.
OrthoDBEOG63Z74X.
ProtClustDBCLSK2504562.

Enzyme and pathway databases

BioCycRLEG216596:GKE5-2847-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL1_RHIL3
AccessionPrimary (citable) accession number: Q1MFI6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 30, 2006
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries