Reviewed, UniProtKB/Swiss-Prot Q1MFI6 (SYL1_RHIL3)

Names and origin

Protein names

Recommended name:
    Leucyl-tRNA synthetase 1
    EC=6.1.1.4
Alternative name(s):
    Leucine--tRNA ligase 1
      Short name=LeuRS 1

Gene names

Name:	leuS1
Ordered Locus Names:	RL2798

Organism

Rhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP]

Taxonomic identifier

216596 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	838 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP MF_00049
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 838	838	Leucyl-tRNA synthetase 1 HAMAP MF_00049		PRO_0000334802
Regions
	Motif	40 – 51	12	"HIGH" region HAMAP MF_00049
	Motif	608 – 612	5	"KMSKS" region HAMAP MF_00049
Sites
	Binding site	611	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1MFI6-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: 7C2AC9CEACE16F9F	FASTA	838	94,069
	10 20 30 40 50 60 MPYDPTKIEP KWQAYWAGHH IFRVEIDPAR PKFYALDMFP YPSGAGLHVG HPLGYTATDI 70 80 90 100 110 120 MCRYKRMRGF NVLHPMGWDS FGLPAERHAM RTGVHPDITT KRNIETFRGQ VQRLGFSYDW 130 140 150 160 170 180 SREFATTDPA YVRWTQWIFL KLFENGLAYQ AEVAVNWCPA QNAVLADEEV KDGRYVETGD 190 200 210 220 230 240 PVIRRRMRQW MLRITAYADR LLQGLDGLDW PENLKAMQRN WIGRSEGAEI RFPLEHGKGV 250 260 270 280 290 300 ITVFTTRPET LFGASYILLA PEHPAVAAIV EPEMSEAVAA YIAEAEGLEE TVRADAGREK 310 320 330 340 350 360 TGVFTGAYAI NPANGARLPV WVADYVLAGY GTGALMAVPA HDARDYAFAH SHELPIIRVI 370 380 390 400 410 420 DSEADIEKGA YEGEGAMVNS GFLSGLGSPE ARSAIVAWLQ ANGTGWPKVM YRLRDWLFSR 430 440 450 460 470 480 QRYWGEPIPV LHLADGSVMP LPEECLPLLP PELDDYAPTP DGEPPLARAQ AWVETIVPGT 490 500 510 520 530 540 DIPARRETNT MPQWAGSCWY YLRFLDPENT SEPVGREAER YWMPVDLYVG GAEHAVLHLL 550 560 570 580 590 600 YARFWHKVLY DIGVVSTEEP FQRLFNQGMI LAHSYRDAAG RYYAPSSVVE EEGRWFAGSV 610 620 630 640 650 660 EVQRAVEKMS KSQLNVVNPD DVVHQFGADA LRLYEMFMGP LDAAKPWQTA GVIGVRRFLE 670 680 690 700 710 720 RAWRIVCDES DGLSPVVLEA APSPQLLRLR HQTVKTVTAD IEAIRFNTAV SRLMELANAL 730 740 750 760 770 780 TAEAARPREV VETFVLLLAP FAPHIAEELW SKLGHGETLT WVSWPTFDPA LIEMETREYV 790 800 810 820 830 VQINGKVRHR FEAAADLGEA LLAAARSEPS VMALLDGKTV VKEVLVPGRL VNFVVEDL

References

[1]

"The genome of Rhizobium leguminosarum has recognizable core and accessory components." Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F., Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H., East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I., Chillingworth T., Clarke K. , Cronin A., Davis P., Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J. Genome Biol. 7:RESEARCH34.1-RESEARCH34.20(2006) [PubMed: 16640791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM236080 Genomic DNA. Translation: CAK08288.1.
RefSeq	YP_768383.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4400999.
GenomeReviews	Gene locus RL2798 in contig AM236080_GR.
KEGG	rle:RL2798.
NMPDR	fig|216596.1.peg.2894.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1MFI6.
OMA	Q1MFI6. YYLRFID.
Family and domain databases
HAMAP	MF_00049. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-synth_Ia_bac/mito. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF7. Leu_tRNAsyn_1a. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL1_RHIL3

Accession

Primary (citable) accession number: Q1MFI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 30, 2006
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)