Reviewed, UniProtKB/Swiss-Prot Q1MBS9 (GLGB1_RHIL3)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme 1
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme 1
      Short name=BE 1
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 1

Gene names

Name:	glgB1
Ordered Locus Names:	RL4115

Organism

Rhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP]

Taxonomic identifier

216596 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	1,4-alpha-glucan-branching enzyme 1 HAMAP MF_00685		PRO_0000260684
Sites
	Active site	313	1	By similarity
	Active site	348	1	By similarity
	Active site	353	1	By similarity
	Active site	416	1	By similarity
	Active site	418	1	By similarity
	Active site	471	1	By similarity
	Active site	538	1	By similarity
	Active site	539	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1MBS9-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: C5146A79B7FCD295	FASTA	735	83,250
	10 20 30 40 50 60 MKTPKTVPEV KLSWEISADE ITAILAGSHS NPFAVLGVHQ AGDAFVARCF IPGAEEVTAM 70 80 90 100 110 120 TLDGGVIGEL KQLHADGVFA GPVSLTKLQP VRYRARRGDA EWAVTDPYSF GPVLGPMDDY 130 140 150 160 170 180 FAREGSHLRL FDKMGAHLIK HDGAQGIHFA VWAPNAQRVS VVGDFNNWDG RRHVMRFRSD 190 200 210 220 230 240 SGIWEIFAPD VPIGVAYKFE IRGQDGVLLP LKADPFARRS ELRPKTASVT AAELEQEWED 250 260 270 280 290 300 EAHLKHWRET DKRRQPISIY EVHAASWQRR QDGTMLSWDE LASSLIPYCA DMGFTHIEFL 310 320 330 340 350 360 PITEYPYDPS WGYQTTGLYA PTARFGEPEG FARFVNGCHK VGIGVILDWV PAHFPTDEHG 370 380 390 400 410 420 LGWFDGTALY EHEDPRKGFH PDWSTAIYNF GRTEVVSYLV NNALYWAEKF HLDGLRVDAV 430 440 450 460 470 480 ASMLYLDYSR KHGEWIPNEY GGNENLEAVR FLQDLNIRIY GKNSNVMTIA EESTSWPKVS 490 500 510 520 530 540 QPVHEGGLGF GFKWNMGFMH DTLSYMSRDP IYRGHHHNEL TFGLLYAYSE NFVLPLSHDE 550 560 570 580 590 600 VVHGKGSLIA KMPGDDWQKF ANLRAYYAYM WGYPGKKLLF MGQEFAQWSE WSEEKALDWN 610 620 630 640 650 660 LLQYRMHEGM RRLVRDLNFT YRSKPALHAR DCEGEGFEWL VADDHQNSVF AWLRKAPGQK 670 680 690 700 710 720 PVAVITNFTP IYRENYSIRL PSAGRWREIL NTDADIYGGS GKGNGGRVQA VDAGGNITCS 730 ITLPPLATIM LEPEN

References

[1]

"The genome of Rhizobium leguminosarum has recognizable core and accessory components." Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F., Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H., East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I., Chillingworth T., Clarke K. , Cronin A., Davis P., Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J. Genome Biol. 7:R34.1-R34.20(2006) [PubMed: 16640791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM236080 Genomic DNA. Translation: CAK09604.1.
RefSeq	YP_769690.1.
3D structure databases
SMR	Q1MBS9. Positions 126-735.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1MBS9.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	4400345.
GenomeReviews	Gene locus RL4115 in contig AM236080_GR.
KEGG	rle:RL4115.
NMPDR	fig|216596.1.peg.4249.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	PPLATIM.
PhylomeDB	Q1MBS9.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB1_RHIL3

Accession

Primary (citable) accession number: Q1MBS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	May 30, 2006
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)