Reviewed, UniProtKB/Swiss-Prot Q1MAN9 (ASSY2_RHIL3)

Names and origin

Protein names

Recommended name:
    Argininosuccinate synthase 2
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase 2

Gene names

Name:	argG2
Ordered Locus Names:	RL4515

Organism

Rhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP]

Taxonomic identifier

216596 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005
Subunit structure	Homotetramer By similarity. HAMAP MF_00005
Subcellular location	Cytoplasm Probable HAMAP MF_00005.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP argininosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Argininosuccinate synthase 2 HAMAP MF_00005		PRO_0000263962
Regions
	Nucleotide binding	13 – 21	9	ATP By similarity
Sites
	Binding site	40	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	91	1	Citrulline By similarity
	Binding site	96	1	Citrulline By similarity
	Binding site	121	1	ATP; via amide nitrogen By similarity
	Binding site	123	1	Aspartate By similarity
	Binding site	127	1	Aspartate By similarity
	Binding site	127	1	Citrulline By similarity
	Binding site	128	1	Aspartate By similarity
	Binding site	131	1	Citrulline By similarity
	Binding site	182	1	Citrulline By similarity
	Binding site	191	1	Citrulline By similarity
	Binding site	267	1	Citrulline By similarity
	Binding site	279	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1MAN9-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: FD48DFBDB2D285DD	FASTA	407	45,177
	10 20 30 40 50 60 MASYKDVKKV VLAYSGGLDT SIILKWLQTE LGAEVVTFTA DLGQGEELEP ARKKAEMLGI 70 80 90 100 110 120 KEIYIEDVRE EFVRDFVFPM FRANAVYEGV YLLGTSIARP LISKHLIDIA KKTGADAIAH 130 140 150 160 170 180 GATGKGNDQV RFELSAYALN PDIKIIAPWR DWAFKSRTDL LAFAEQHQIP VAKDKMGEAP 190 200 210 220 230 240 FSVDANLLHS SSEGKVLEDP SQEAPEYVHM RTISPEAAPD KATTIKVGFE KGDAVSINGV 250 260 270 280 290 300 RMSPATLLAA LNNYGRDNGI GRLDLVENRF VGMKSRGVYE TPGGTILLTA HRAIESITLD 310 320 330 340 350 360 RGAAHLKDDI MPRYAELIYY GFWFSPEREM LQALIDKSQE HVEGEVTLKL YKGNVMVIGR 370 380 390 400 ESDKSLYSDK LVTFEDDQGA YDQKDAAGFI KLNALRLRTL AKRNLVK

References

[1]

"The genome of Rhizobium leguminosarum has recognizable core and accessory components." Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F., Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H., East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I., Chillingworth T., Clarke K. , Cronin A., Davis P., Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J. Genome Biol. 7:R34.1-R34.20(2006) [PubMed: 16640791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM236080 Genomic DNA. Translation: CAK09999.1.
RefSeq	YP_770080.1.
3D structure databases
SMR	Q1MAN9. Positions 9-401.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1MAN9.
Genome annotation databases
GeneID	4400741.
GenomeReviews	Gene locus RL4515 in contig AM236080_GR.
KEGG	rle:RL4515.
NMPDR	fig|216596.1.peg.4648.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0137.
HOGENOM	HBG335267.
OMA	PLIAKRQ.
PhylomeDB	Q1MAN9.
Family and domain databases
HAMAP	MF_00005. Arg_succ_synth_type1. [Tree]
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY2_RHIL3

Accession

Primary (citable) accession number: Q1MAN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	May 30, 2006
Last modified:	February 9, 2010
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)