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Protein

Leucine--tRNA ligase 2

Gene

leuS2

Organism
Rhizobium leguminosarum bv. viciae (strain 3841)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei635 – 6351ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRLEG216596:GKE5-4809-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase 2UniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetase 2UniRule annotation
Short name:
LeuRS 2UniRule annotation
Gene namesi
Name:leuS2UniRule annotation
Ordered Locus Names:RL4732
OrganismiRhizobium leguminosarum bv. viciae (strain 3841)
Taxonomic identifieri216596 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
ProteomesiUP000006575 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 876876Leucine--tRNA ligase 2PRO_0000334803Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi216596.RL4732.

Structurei

3D structure databases

ProteinModelPortaliQ1MA25.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi632 – 6365"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiFFDSAWY.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1MA25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATERYNPRD AEPRWQQKWN EDKVFETDNA DPREKYYVLE MFPYPSGRIH
60 70 80 90 100
MGHVRNYAMG DVVARYKRAR GYNVLHPMGW DAFGMPAENA AMERGVHPAS
110 120 130 140 150
WTYQNIGSMK AQLKAMGLSL DWSREFATCD VEYYQHQQHL FLDFLEKGLV
160 170 180 190 200
YRKQSKVNWD PVDNTVLANE QVIDGRGWRS GALVEQRELT QWFFKITDFS
210 220 230 240 250
QDLLDALDTL DQWPEKVRLM QKNWIGRSEG LTIRWEIVPE TAPAGESEVT
260 270 280 290 300
VYTTRPDTLF GASFLAIAAD HPLAKDAAAK NPEIEAFCDE CRRAGTSLAA
310 320 330 340 350
LETAEKKGMD TGIRVRHPLD PSWELPVYIA NFVLMDYGTG AIFGCPSGDQ
360 370 380 390 400
RDLDFARKYG LPVVAVVMPR DGDAASFSVG DTAYDGDGVM INSRFLDGKT
410 420 430 440 450
TEEAFNIVAD RLSAASLGNA PQGERKVNFR LRDWGISRQR YWGCPIPVIH
460 470 480 490 500
CDDCGVVPVP KADLPVKLPD DVTFDQPGNP LDRHPTWRHV SCPNCGKDAR
510 520 530 540 550
RETDTMDTFV DSSWYFTRFT APWEDKPTDP EAANRWLPVD QYIGGIEHAI
560 570 580 590 600
LHLLYSRFFT RAMRETGHVA ATEPFKGLFT QGMVVHETYS RGAGASREWV
610 620 630 640 650
APADIRIDEL DGKRRAFLLT NNEEVSIGSI EKMSKSKKNV VDPDDIIASY
660 670 680 690 700
GADTARFFVL SDSPPERDVI WSEAGVEGAH RFTQRLWRLI SEAADALSAV
710 720 730 740 750
APAPATDGEA LSISQAAHKT LKAVQNDYDK LWFNKAVARI YELVNALAAP
760 770 780 790 800
MTKVAAGEGD ATYRAAVRDA AEILIQLVSP MTPHLAEECW AALGNEGLLA
810 820 830 840 850
RASWPQYDET LVIENSVVLP VQINGKKRAE LTISRDADQN TVTDAVLDLD
860 870
AVKNALNGQA PKKIIVVPQR IVNIVV
Length:876
Mass (Da):97,967
Last modified:May 30, 2006 - v1
Checksum:i65A2A38FF62B870A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM236080 Genomic DNA. Translation: CAK10215.1.
RefSeqiYP_770294.1. NC_008380.1.

Genome annotation databases

EnsemblBacteriaiCAK10215; CAK10215; RL4732.
KEGGirle:RL4732.
PATRICi23146360. VBIRhiLeg32091_6079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM236080 Genomic DNA. Translation: CAK10215.1.
RefSeqiYP_770294.1. NC_008380.1.

3D structure databases

ProteinModelPortaliQ1MA25.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216596.RL4732.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAK10215; CAK10215; RL4732.
KEGGirle:RL4732.
PATRICi23146360. VBIRhiLeg32091_6079.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiFFDSAWY.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciRLEG216596:GKE5-4809-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 3841.

Entry informationi

Entry nameiSYL2_RHIL3
AccessioniPrimary (citable) accession number: Q1MA25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 30, 2006
Last modified: April 29, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.