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Q1M3A7

- GLGB2_RHIL3

UniProt

Q1M3A7 - GLGB2_RHIL3

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Protein
1,4-alpha-glucan branching enzyme GlgB 2
Gene
glgB2, pRL120710
Organism
Rhizobium leguminosarum bv. viciae (strain 3841)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei415 – 4151Nucleophile By similarity
Active sitei468 – 4681Proton donor By similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-4883-MONOMER.
RLEG216596:GKE5-5525-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB 2 (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2
Alpha-(1->4)-glucan branching enzyme 2
Glycogen branching enzyme 2
Short name:
BE 2
Gene namesi
Name:glgB2
Ordered Locus Names:pRL120710
OrganismiRhizobium leguminosarum bv. viciae (strain 3841)
Taxonomic identifieri216596 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
ProteomesiUP000006575: Plasmid pRL12

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7367361,4-alpha-glucan branching enzyme GlgB 2UniRule annotation
PRO_0000260685Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi216596.pRL120710.

Structurei

3D structure databases

ProteinModelPortaliQ1M3A7.
SMRiQ1M3A7. Positions 121-734.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiHERCPHA.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1M3A7-1 [UniParc]FASTAAdd to Basket

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MNVERSELLA GIGQDALWAL IEGRHGDPFS ILGPHQSGGM TIVRVYLPGA    50
EAVDLIDATS GRVVAPFSIA HPSGLFAATV ASRTGYRLRI TWPDAVQITE 100
DPYSFGLLLG ELDLHLISEG THYSLSRTLG AVAMSIDGIS GVRFAVWAPN 150
ARRVSVVGDF NAWDGRRNPM RLRPSAGVWE LFIPRLAPGE RYKFEIVDAE 200
GTCLPQKADP VARASEAAPS TASIVASSTP FRWTDDGWMK GRSRQDRLEG 250
AFSVYEVHVG SWLRDQKDGN RSLDWVELSQ RLVPYVSDMG FTHIELLPIM 300
EHPFGGSWGY QPLGLFAPTG RYGTPEDFAY FVDRCHGAGL GVILDWVPAH 350
FPTDVWGLAR FDGSALYEHE DPREGFHRDW NTLIYNLGRN EVKGFLIASA 400
LEWLERYHID GLRVDAVASM LYRDYSRNEG EWIPNQYGGR ENLEAVEFFK 450
HLNSIIHERC PHAMTIAEES TAWPGVTKPP EQGGLGFDIK WNMGWMHDSL 500
SYIEKDPIYR SYAHGTMTFG MIYAYSERFI LPISHDEVVY GKGSLLTKMP 550
GDEWQKFANL RSYLAFMWGH PGKKLLFMGS EIAQPSEWNH DGSVTWDVLD 600
QPQHVGIQRL VKDLNGLYGD EPALQFGDFH SEGFEWAAAD DAVNSVLGML 650
RYAPDRASSV LVMSNFTPVP RYGYRIGVPS DGVWIERITT DAREYGGSGL 700
VNGAVSSEPV PAHGRPVSLS LTLPPLSTIF LQGPSP 736
Length:736
Mass (Da):81,853
Last modified:May 30, 2006 - v1
Checksum:iF81E22D2BE2DA1CA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM236086 Genomic DNA. Translation: CAK12419.1.
RefSeqiYP_765213.1. NC_008378.1.

Genome annotation databases

EnsemblBacteriaiCAK12419; CAK12419; pRL120710.
GeneIDi4398207.
KEGGirle:pRL120710.
PATRICi23135507. VBIRhiLeg32091_0715.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM236086 Genomic DNA. Translation: CAK12419.1 .
RefSeqi YP_765213.1. NC_008378.1.

3D structure databases

ProteinModelPortali Q1M3A7.
SMRi Q1M3A7. Positions 121-734.
ModBasei Search...

Protein-protein interaction databases

STRINGi 216596.pRL120710.

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAK12419 ; CAK12419 ; pRL120710 .
GeneIDi 4398207.
KEGGi rle:pRL120710.
PATRICi 23135507. VBIRhiLeg32091_0715.

Phylogenomic databases

eggNOGi COG0296.
HOGENOMi HOG000283037.
KOi K00700.
OMAi HERCPHA.
OrthoDBi EOG6JX7GT.

Enzyme and pathway databases

UniPathwayi UPA00164 .
BioCyci RETL1328306-WGS:GSTH-4883-MONOMER.
RLEG216596:GKE5-5525-MONOMER.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 3841.

Entry informationi

Entry nameiGLGB2_RHIL3
AccessioniPrimary (citable) accession number: Q1M3A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: May 30, 2006
Last modified: July 9, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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