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Q1M3A7

- GLGB2_RHIL3

UniProt

Q1M3A7 - GLGB2_RHIL3

Protein

1,4-alpha-glucan branching enzyme GlgB 2

Gene

glgB2

Organism
Rhizobium leguminosarum bv. viciae (strain 3841)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (30 May 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei415 – 4151NucleophileUniRule annotation
    Active sitei468 – 4681Proton donorUniRule annotation

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-4883-MONOMER.
    RLEG216596:GKE5-5525-MONOMER.
    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan branching enzyme GlgB 2UniRule annotation (EC:2.4.1.18UniRule annotation)
    Alternative name(s):
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2UniRule annotation
    Alpha-(1->4)-glucan branching enzyme 2UniRule annotation
    Glycogen branching enzyme 2UniRule annotation
    Short name:
    BE 2UniRule annotation
    Gene namesi
    Name:glgB2UniRule annotation
    Ordered Locus Names:pRL120710
    OrganismiRhizobium leguminosarum bv. viciae (strain 3841)
    Taxonomic identifieri216596 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
    ProteomesiUP000006575: Plasmid pRL12

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7367361,4-alpha-glucan branching enzyme GlgB 2PRO_0000260685Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi216596.pRL120710.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1M3A7.
    SMRiQ1M3A7. Positions 121-734.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0296.
    HOGENOMiHOG000283037.
    KOiK00700.
    OMAiHERCPHA.
    OrthoDBiEOG6JX7GT.

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_00685. GlgB.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q1M3A7-1 [UniParc]FASTAAdd to Basket

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    MNVERSELLA GIGQDALWAL IEGRHGDPFS ILGPHQSGGM TIVRVYLPGA    50
    EAVDLIDATS GRVVAPFSIA HPSGLFAATV ASRTGYRLRI TWPDAVQITE 100
    DPYSFGLLLG ELDLHLISEG THYSLSRTLG AVAMSIDGIS GVRFAVWAPN 150
    ARRVSVVGDF NAWDGRRNPM RLRPSAGVWE LFIPRLAPGE RYKFEIVDAE 200
    GTCLPQKADP VARASEAAPS TASIVASSTP FRWTDDGWMK GRSRQDRLEG 250
    AFSVYEVHVG SWLRDQKDGN RSLDWVELSQ RLVPYVSDMG FTHIELLPIM 300
    EHPFGGSWGY QPLGLFAPTG RYGTPEDFAY FVDRCHGAGL GVILDWVPAH 350
    FPTDVWGLAR FDGSALYEHE DPREGFHRDW NTLIYNLGRN EVKGFLIASA 400
    LEWLERYHID GLRVDAVASM LYRDYSRNEG EWIPNQYGGR ENLEAVEFFK 450
    HLNSIIHERC PHAMTIAEES TAWPGVTKPP EQGGLGFDIK WNMGWMHDSL 500
    SYIEKDPIYR SYAHGTMTFG MIYAYSERFI LPISHDEVVY GKGSLLTKMP 550
    GDEWQKFANL RSYLAFMWGH PGKKLLFMGS EIAQPSEWNH DGSVTWDVLD 600
    QPQHVGIQRL VKDLNGLYGD EPALQFGDFH SEGFEWAAAD DAVNSVLGML 650
    RYAPDRASSV LVMSNFTPVP RYGYRIGVPS DGVWIERITT DAREYGGSGL 700
    VNGAVSSEPV PAHGRPVSLS LTLPPLSTIF LQGPSP 736
    Length:736
    Mass (Da):81,853
    Last modified:May 30, 2006 - v1
    Checksum:iF81E22D2BE2DA1CA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM236086 Genomic DNA. Translation: CAK12419.1.
    RefSeqiYP_765213.1. NC_008378.1.

    Genome annotation databases

    EnsemblBacteriaiCAK12419; CAK12419; pRL120710.
    GeneIDi4398207.
    KEGGirle:pRL120710.
    PATRICi23135507. VBIRhiLeg32091_0715.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM236086 Genomic DNA. Translation: CAK12419.1 .
    RefSeqi YP_765213.1. NC_008378.1.

    3D structure databases

    ProteinModelPortali Q1M3A7.
    SMRi Q1M3A7. Positions 121-734.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 216596.pRL120710.

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAK12419 ; CAK12419 ; pRL120710 .
    GeneIDi 4398207.
    KEGGi rle:pRL120710.
    PATRICi 23135507. VBIRhiLeg32091_0715.

    Phylogenomic databases

    eggNOGi COG0296.
    HOGENOMi HOG000283037.
    KOi K00700.
    OMAi HERCPHA.
    OrthoDBi EOG6JX7GT.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .
    BioCyci RETL1328306-WGS:GSTH-4883-MONOMER.
    RLEG216596:GKE5-5525-MONOMER.

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_00685. GlgB.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 3841.

    Entry informationi

    Entry nameiGLGB2_RHIL3
    AccessioniPrimary (citable) accession number: Q1M3A7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: May 30, 2006
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3