glgB2

Functionⁱ

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityⁱ

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayⁱ: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	415	NucleophileUniRule annotation		1
Active siteⁱ	468	Proton donorUniRule annotation		1

GO - Molecular functionⁱ

1,4-alpha-glucan branching enzyme activity Source: UniProtKB-EC
cation binding Source: InterPro
hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

Complete GO annotation...

GO - Biological processⁱ

glycogen biosynthetic process Source: UniProtKB-UniPathway

Complete GO annotation...

Keywordsⁱ

Molecular function	Glycosyltransferase, Transferase
Biological process	Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayⁱ	UPA00164.

UniPathwayⁱ

UPA00164.

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.

CAZyⁱ

CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 1,4-alpha-glucan branching enzyme GlgB 2UniRule annotation (EC:2.4.1.18UniRule annotation) Alternative name(s): 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2UniRule annotation Alpha-(1->4)-glucan branching enzyme 2UniRule annotation Glycogen branching enzyme 2UniRule annotation Short name: BE 2UniRule annotation
Gene namesⁱ	Name:glgB2UniRule annotation Ordered Locus Names:pRL120710
Organismⁱ	Rhizobium leguminosarum bv. viciae (strain 3841)
Taxonomic identifierⁱ	216596 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium › Rhizobium leguminosarum › Rhizobium leguminosarum bv. viciae
Proteomesⁱ	UP000006575 Componentⁱ: Plasmid pRL12

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000260685	1 – 736	1,4-alpha-glucan branching enzyme GlgB 2Add BLAST		736

Interactionⁱ

Subunit structureⁱ

Monomer.UniRule annotation

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q1M3A7.
SMRⁱ	Q1M3A7.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

HOGENOMⁱ	HOG000283037.
KEGG Orthology (KO) More...KOⁱ	K00700.
OMAⁱ	EVVHGKS.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H08D1.

Family and domain databases

Gene3Dⁱ	2.60.40.10. 2 hits.
HAMAPⁱ	MF_00685. GlgB. 1 hit.
InterProⁱ	View protein in InterPro IPR006048. A-amylase/branching_C. IPR006407. GlgB. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit.
PIRSFⁱ	PIRSF000463. GlgB. 1 hit.
SMARTⁱ	View protein in SMART SM00642. Aamy. 1 hit.
SUPFAMⁱ	SSF51445. SSF51445. 1 hit. SSF81296. SSF81296. 2 hits.
TIGRFAMsⁱ	TIGR01515. branching_enzym. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q1M3A7-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNVERSELLA GIGQDALWAL IEGRHGDPFS ILGPHQSGGM TIVRVYLPGA 
        60         70         80         90        100
EAVDLIDATS GRVVAPFSIA HPSGLFAATV ASRTGYRLRI TWPDAVQITE 
       110        120        130        140        150
DPYSFGLLLG ELDLHLISEG THYSLSRTLG AVAMSIDGIS GVRFAVWAPN 
       160        170        180        190        200
ARRVSVVGDF NAWDGRRNPM RLRPSAGVWE LFIPRLAPGE RYKFEIVDAE 
       210        220        230        240        250
GTCLPQKADP VARASEAAPS TASIVASSTP FRWTDDGWMK GRSRQDRLEG 
       260        270        280        290        300
AFSVYEVHVG SWLRDQKDGN RSLDWVELSQ RLVPYVSDMG FTHIELLPIM 
       310        320        330        340        350
EHPFGGSWGY QPLGLFAPTG RYGTPEDFAY FVDRCHGAGL GVILDWVPAH 
       360        370        380        390        400
FPTDVWGLAR FDGSALYEHE DPREGFHRDW NTLIYNLGRN EVKGFLIASA 
       410        420        430        440        450
LEWLERYHID GLRVDAVASM LYRDYSRNEG EWIPNQYGGR ENLEAVEFFK 
       460        470        480        490        500
HLNSIIHERC PHAMTIAEES TAWPGVTKPP EQGGLGFDIK WNMGWMHDSL 
       510        520        530        540        550
SYIEKDPIYR SYAHGTMTFG MIYAYSERFI LPISHDEVVY GKGSLLTKMP 
       560        570        580        590        600
GDEWQKFANL RSYLAFMWGH PGKKLLFMGS EIAQPSEWNH DGSVTWDVLD 
       610        620        630        640        650
QPQHVGIQRL VKDLNGLYGD EPALQFGDFH SEGFEWAAAD DAVNSVLGML 
       660        670        680        690        700
RYAPDRASSV LVMSNFTPVP RYGYRIGVPS DGVWIERITT DAREYGGSGL 
       710        720        730 
VNGAVSSEPV PAHGRPVSLS LTLPPLSTIF LQGPSP

Length:736

Mass (Da):81,853

Last modified:May 30, 2006 - v1

Checksum:ⁱF81E22D2BE2DA1CA

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AM236086 Genomic DNA. Translation: CAK12419.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011649463.1. NC_008378.1.

Genome annotation databases

EnsemblBacteriaⁱ	CAK12419; CAK12419; pRL120710.
KEGGⁱ	rle:pRL120710.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AM236086 Genomic DNA. Translation: CAK12419.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011649463.1. NC_008378.1.

3D structure databases

ProteinModelPortalⁱ	Q1M3A7.
SMRⁱ	Q1M3A7.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.

CAZyⁱ

CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	CAK12419; CAK12419; pRL120710.
KEGGⁱ	rle:pRL120710.

Phylogenomic databases

HOGENOMⁱ	HOG000283037.
KEGG Orthology (KO) More...KOⁱ	K00700.
OMAⁱ	EVVHGKS.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H08D1.

Enzyme and pathway databases

UniPathwayⁱ	UPA00164.

UniPathwayⁱ

UPA00164.

Family and domain databases

Gene3Dⁱ	2.60.40.10. 2 hits.
HAMAPⁱ	MF_00685. GlgB. 1 hit.
InterProⁱ	View protein in InterPro IPR006048. A-amylase/branching_C. IPR006407. GlgB. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit.
PIRSFⁱ	PIRSF000463. GlgB. 1 hit.
SMARTⁱ	View protein in SMART SM00642. Aamy. 1 hit.
SUPFAMⁱ	SSF51445. SSF51445. 1 hit. SSF81296. SSF81296. 2 hits.
TIGRFAMsⁱ	TIGR01515. branching_enzym. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GLGB2_RHIL3
Accessionⁱ	Q1M3A7Primary (citable) accession number: Q1M3A7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
	Last sequence update:	May 30, 2006
	Last modified:	June 7, 2017
	This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome

Documents

Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries
PATHWAY comments
Index of metabolic and biosynthesis pathways
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q1M3A7 (GLGB2_RHIL3)

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1,4-alpha-glucan branching enzyme GlgB 2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glycogen biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

Pathwayⁱ: glycogen biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ