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Q1M3A7 (GLGB2_RHIL3) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB 2

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2
Alpha-(1->4)-glucan branching enzyme 2
Glycogen branching enzyme 2
Short name=BE 2
Gene names
Name:glgB2
Ordered Locus Names:pRL120710
OrganismRhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP]
Taxonomic identifier216596 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Protein attributes

Sequence length736 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7367361,4-alpha-glucan branching enzyme GlgB 2 HAMAP-Rule MF_00685
PRO_0000260685

Sites

Active site4151Nucleophile By similarity
Active site4681Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1M3A7 [UniParc].

Last modified May 30, 2006. Version 1.
Checksum: F81E22D2BE2DA1CA

FASTA73681,853
        10         20         30         40         50         60 
MNVERSELLA GIGQDALWAL IEGRHGDPFS ILGPHQSGGM TIVRVYLPGA EAVDLIDATS 

        70         80         90        100        110        120 
GRVVAPFSIA HPSGLFAATV ASRTGYRLRI TWPDAVQITE DPYSFGLLLG ELDLHLISEG 

       130        140        150        160        170        180 
THYSLSRTLG AVAMSIDGIS GVRFAVWAPN ARRVSVVGDF NAWDGRRNPM RLRPSAGVWE 

       190        200        210        220        230        240 
LFIPRLAPGE RYKFEIVDAE GTCLPQKADP VARASEAAPS TASIVASSTP FRWTDDGWMK 

       250        260        270        280        290        300 
GRSRQDRLEG AFSVYEVHVG SWLRDQKDGN RSLDWVELSQ RLVPYVSDMG FTHIELLPIM 

       310        320        330        340        350        360 
EHPFGGSWGY QPLGLFAPTG RYGTPEDFAY FVDRCHGAGL GVILDWVPAH FPTDVWGLAR 

       370        380        390        400        410        420 
FDGSALYEHE DPREGFHRDW NTLIYNLGRN EVKGFLIASA LEWLERYHID GLRVDAVASM 

       430        440        450        460        470        480 
LYRDYSRNEG EWIPNQYGGR ENLEAVEFFK HLNSIIHERC PHAMTIAEES TAWPGVTKPP 

       490        500        510        520        530        540 
EQGGLGFDIK WNMGWMHDSL SYIEKDPIYR SYAHGTMTFG MIYAYSERFI LPISHDEVVY 

       550        560        570        580        590        600 
GKGSLLTKMP GDEWQKFANL RSYLAFMWGH PGKKLLFMGS EIAQPSEWNH DGSVTWDVLD 

       610        620        630        640        650        660 
QPQHVGIQRL VKDLNGLYGD EPALQFGDFH SEGFEWAAAD DAVNSVLGML RYAPDRASSV 

       670        680        690        700        710        720 
LVMSNFTPVP RYGYRIGVPS DGVWIERITT DAREYGGSGL VNGAVSSEPV PAHGRPVSLS 

       730 
LTLPPLSTIF LQGPSP 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM236086 Genomic DNA. Translation: CAK12419.1.
RefSeqYP_765213.1. NC_008378.1.

3D structure databases

ProteinModelPortalQ1M3A7.
SMRQ1M3A7. Positions 121-734.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING216596.pRL120710.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAK12419; CAK12419; pRL120710.
GeneID4398207.
KEGGrle:pRL120710.
PATRIC23135507. VBIRhiLeg32091_0715.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAHERCPHA.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycRETL1328306-WGS:GSTH-4883-MONOMER.
RLEG216596:GKE5-5525-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB2_RHIL3
AccessionPrimary (citable) accession number: Q1M3A7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: May 30, 2006
Last modified: July 9, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries