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Q1M0V6

- GCNT3_BUBBU

UniProt

Q1M0V6 - GCNT3_BUBBU

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Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Gene

GCNT3

Organism
Bubalus bubalis (Domestic water buffalo)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

Pathwayi

GO - Molecular functioni

  1. beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
  2. N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (EC:2.4.1.102, EC:2.4.1.150)
Alternative name(s):
C2GnT-mucin type
Short name:
C2GnT-M
Gene namesi
Name:GCNT3
OrganismiBubalus bubalis (Domestic water buffalo)
Taxonomic identifieri89462 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBubalus

Subcellular locationi

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3PRO_0000288544Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi73 ↔ 230By similarity
Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi164 ↔ 384By similarity
Disulfide bondi185 ↔ 212By similarity
Disulfide bondi393 ↔ 425By similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ1M0V6.
SMRiQ1M0V6. Positions 70-435.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini31 – 440410LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei13 – 3018Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 14 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG051711.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1M0V6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKMTGWKKKL CRGHHLWALG CYMLLAVVAL RLSLRLKCDV DSLDLESRDF
60 70 80 90 100
QSQRCRDVLY KNLKLPAKRS INCSGITRGD QEAVVQALLD NLEVKKKRLP
110 120 130 140 150
FTDTYYLNIT RDCERFKAQR KFIQFPLSKE ELDFPIAYSM VVHEKIENFE
160 170 180 190 200
RLLRAVYAPQ NIYCVHVDVK SPEAFKEAVK AIISCFPNVF MASKLVPVVY
210 220 230 240 250
ASWSRVQADL NCMEDLLQSS VPWKYLLNTC GTDFPIKTNA EMVLALKMLN
260 270 280 290 300
GKNSMESEIP SEYKKNRWKY RYEVTDRLYL TSKMKDPPPD NLPMFTGNAY
310 320 330 340 350
FVASRAFVQH VLENPKSQRL IEWVKDTYSP DEHLWATLQR APWMPGSVPY
360 370 380 390 400
HPKYHISDMT AIARLVKWQN HEGDVSMGAP YAPCSGIHQR AICIYGAGDL
410 420 430 440
HWILQNHHLL ANKFDPRVDD NVLQCLEEYL RHKAIYGTEL
Length:440
Mass (Da):51,008
Last modified:May 30, 2006 - v1
Checksum:i8F25E256DC7A5291
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti115 – 1151R → Q in strain: River-type.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY847312 Genomic DNA. Translation: AAY21831.1.
AY847313 Genomic DNA. Translation: AAY21832.1.
AY847321 Genomic DNA. Translation: AAY21840.1.
RefSeqiXP_006072400.1. XM_006072338.1.

Genome annotation databases

GeneIDi102398238.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY847312 Genomic DNA. Translation: AAY21831.1 .
AY847313 Genomic DNA. Translation: AAY21832.1 .
AY847321 Genomic DNA. Translation: AAY21840.1 .
RefSeqi XP_006072400.1. XM_006072338.1.

3D structure databases

ProteinModelPortali Q1M0V6.
SMRi Q1M0V6. Positions 70-435.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT14. Glycosyltransferase Family 14.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 102398238.

Organism-specific databases

CTDi 9245.

Phylogenomic databases

HOVERGENi HBG051711.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Family and domain databases

InterProi IPR003406. Glyco_trans_14.
[Graphical view ]
Pfami PF02485. Branch. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Phylogeographical analysis of bovine herpesvirus 4: demonstration that inter-strain recombination events took place after acquisition of the Bo17 gene from an ancestor of the African buffalo."
    Dewals B., Markine-Goriaynoff N., de Fays K., Minner F., Daix V., Vercammen F., Gaillard C., Sharp P.M., Vanderplasschen A.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Murrah, River-type and Swamp-type.

Entry informationi

Entry nameiGCNT3_BUBBU
AccessioniPrimary (citable) accession number: Q1M0V6
Secondary accession number(s): Q1M0W4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 30, 2006
Last modified: October 29, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3