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Q1LZA3

- ASNS_BOVIN

UniProt

Q1LZA3 - ASNS_BOVIN

Protein

Asparagine synthetase [glutamine-hydrolyzing]

Gene

ASNS

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei97 – 971GlutamineBy similarity
    Binding sitei256 – 2561ATP; via carbonyl oxygenBy similarity
    Binding sitei288 – 2881ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Sitei365 – 3651Important for beta-aspartyl-AMP intermediate formationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi363 – 3642ATPBy similarity

    GO - Molecular functioni

    1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
    3. negative regulation of apoptotic process Source: Ensembl
    4. positive regulation of mitotic cell cycle Source: Ensembl

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Asparagine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_204438. ATF4 activates genes.
    REACT_217400. Amino acid synthesis and interconversion (transamination).
    UniPathwayiUPA00134; UER00195.

    Protein family/group databases

    MEROPSiC44.974.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
    Alternative name(s):
    Glutamine-dependent asparagine synthetase
    Gene namesi
    Name:ASNS
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 4

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 561560Asparagine synthetase [glutamine-hydrolyzing]PRO_0000269563Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei385 – 3851N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ1LZA3.
    PRIDEiQ1LZA3.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1LZA3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 191190Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini213 – 536324Asparagine synthetaseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni49 – 535Glutamine bindingBy similarity
    Regioni75 – 773Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 asparagine synthetase domain.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0367.
    GeneTreeiENSGT00390000001994.
    HOGENOMiHOG000027493.
    HOVERGENiHBG003103.
    InParanoidiQ1LZA3.
    KOiK01953.
    OMAiDFINTAM.
    OrthoDBiEOG75MVVS.
    TreeFamiTF300603.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q1LZA3-1 [UniParc]FASTAAdd to Basket

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    MCGIWALFGS DDCLSVQCLS AMKIAHRGPD AFRFENVNGY TNCCFGFHRL    50
    AVVDQLFGMQ PIRVKKYPYL WLCYNGEIYN HKKLQHHFEF EYQTKVDGEI 100
    ILHLYDKGGI EQTVCMLDGV FAFILLDTAN KKVFLGRDTY GVRPLFKAMT 150
    EDGFLAVCSE AKGLVNLKHS MTPFLKVEPF LPGHYEVLDL KPNGKVASVE 200
    MVKHHHCRDE PLHALYDGVE KLFPGFEIET VKSNLRILFD NAVKKRLMTD 250
    RRIGCLLSGG LDSSLVAATL LKQLKEAQVQ YPLQTFAIGM EDSPDLLAAR 300
    KVANHIGSEH HEVLFNSEEG IQVLDEVIFS LETYDITTVR ASVGMYLISK 350
    YIRKNTDSVV IFSGEGSDEL TQGYIYFHKA PSPEKAEEES ERLLRELYLF 400
    DVLRADRTTA AHGLELRVPF LDHRFSSYYL SLPPDMRVPK NGIEKHLLRE 450
    TFEDSNLIPK EILWRPKEAF SDGITSVKNS WFRILQDYIE HQVDDAAMAS 500
    AAQKFPINTP KTKEGYYYRQ IFENHYPGRA DWLPHYWMPR WTNATDPSAR 550
    TLTHYKAAAK A 561
    Length:561
    Mass (Da):64,220
    Last modified:January 23, 2007 - v3
    Checksum:i71379296556DB3D1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC116123 mRNA. Translation: AAI16124.1.
    RefSeqiNP_001069121.1. NM_001075653.1.
    XP_005205202.1. XM_005205145.1.
    XP_005205203.1. XM_005205146.1.
    UniGeneiBt.61275.

    Genome annotation databases

    EnsembliENSBTAT00000004181; ENSBTAP00000004181; ENSBTAG00000003222.
    GeneIDi514209.
    KEGGibta:514209.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC116123 mRNA. Translation: AAI16124.1 .
    RefSeqi NP_001069121.1. NM_001075653.1.
    XP_005205202.1. XM_005205145.1.
    XP_005205203.1. XM_005205146.1.
    UniGenei Bt.61275.

    3D structure databases

    ProteinModelPortali Q1LZA3.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C44.974.

    Proteomic databases

    PaxDbi Q1LZA3.
    PRIDEi Q1LZA3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000004181 ; ENSBTAP00000004181 ; ENSBTAG00000003222 .
    GeneIDi 514209.
    KEGGi bta:514209.

    Organism-specific databases

    CTDi 440.

    Phylogenomic databases

    eggNOGi COG0367.
    GeneTreei ENSGT00390000001994.
    HOGENOMi HOG000027493.
    HOVERGENi HBG003103.
    InParanoidi Q1LZA3.
    KOi K01953.
    OMAi DFINTAM.
    OrthoDBi EOG75MVVS.
    TreeFami TF300603.

    Enzyme and pathway databases

    UniPathwayi UPA00134 ; UER00195 .
    Reactomei REACT_204438. ATF4 activates genes.
    REACT_217400. Amino acid synthesis and interconversion (transamination).

    Miscellaneous databases

    NextBioi 20871226.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Ascending colon.

    Entry informationi

    Entry nameiASNS_BOVIN
    AccessioniPrimary (citable) accession number: Q1LZA3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 12, 2006
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 67 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3