ID   DNM3A_RAT               Reviewed;         908 AA.
AC   Q1LZ53; Q1LZ52;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   16-JUN-2009, entry version 25.
DE   RecName: Full=DNA (cytosine-5)-methyltransferase 3A;
DE            Short=Dnmt3a;
DE            EC=2.1.1.37;
GN   Name=Dnmt3a;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RX   PubMed=15203217; DOI=10.1016/j.ygeno.2004.02.004;
RA   Lees-Murdock D.J., McLoughlin G.A., McDaid J.R., Quinn L.M.,
RA   O'Doherty A., Hiripi L., Hack C.J., Walsh C.P.;
RT   "Identification of 11 pseudogenes in the DNA methyltransferase gene
RT   family in rodents and humans and implications for the functional
RT   loci.";
RL   Genomics 84:193-204(2004).
CC   -!- FUNCTION: Required for genome wide de novo methylation and is
CC       essential for development. DNA methylation is coordinated with
CC       methylation of histones. It modifies DNA in a non-processive
CC       manner and also methylates non-CpG sites. Acts as a
CC       transcriptional corepressor for ZNF238. Can actively repress
CC       transcription through the recruitment of HDAC activity (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + DNA = S-adenosyl-L-
CC       homocysteine + DNA containing 5-methylcytosine.
CC   -!- SUBUNIT: Interacts with DNMT1 and DNMT3B. Binds the ZNF238
CC       transcriptional repressor. Interacts with SETDB1. Associates with
CC       HDAC1 through its ADD-type zinc-finger (By similarity). Interacts
CC       with the PRC2/EED-EZH2 complex (By similarity).
CC   -!- SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By
CC       similarity).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative promoter usage; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q1LZ53-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q1LZ53-2; Sequence=VSP_029986;
CC   -!- SIMILARITY: Belongs to the C5-methyltransferase family.
CC   -!- SIMILARITY: Contains 1 ADD-type zinc finger.
CC   -!- SIMILARITY: Contains 1 PWWP domain.
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DR   EMBL; BN000395; CAE52317.1; -; mRNA.
DR   EMBL; BN000396; CAE52318.1; -; mRNA.
DR   IPI; IPI00387877; -.
DR   IPI; IPI00760141; -.
DR   RefSeq; NP_001003957.1; -.
DR   RefSeq; NP_001003958.1; -.
DR   UniGene; Rn.92659; -.
DR   PhosphoSite; Q1LZ53; -.
DR   Ensembl; ENSRNOG00000026649; Rattus norvegicus.
DR   GeneID; 444984; -.
DR   KEGG; rno:444984; -.
DR   RGD; 1303336; Dnmt3a.
DR   HOVERGEN; Q1LZ53; -.
DR   BRENDA; 2.1.1.37; 248.
DR   NextBio; 697058; -.
DR   ArrayExpress; Q1LZ53; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IDA:RGD.
DR   GO; GO:0051718; F:DNA (cytosine-5-)-methyltransferase activit...; IDA:RGD.
DR   GO; GO:0045322; F:unmethylated CpG binding; IDA:RGD.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0010424; P:DNA methylation on cytosine within a CG seq...; IDA:RGD.
DR   GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
DR   GO; GO:0046498; P:S-adenosylhomocysteine metabolic process; IDA:RGD.
DR   GO; GO:0046499; P:S-adenosylmethioninamine metabolic process; IDA:RGD.
DR   GO; GO:0006350; P:transcription; IEA:UniProtKB-KW.
DR   InterPro; IPR001525; C5_DNA_meth.
DR   InterPro; IPR018117; C5_DNA_meth_AS.
DR   InterPro; IPR000313; PWWP.
DR   Pfam; PF00145; DNA_methylase; 1.
DR   Pfam; PF00855; PWWP; 1.
DR   SMART; SM00293; PWWP; 1.
DR   PROSITE; PS00094; C5_MTASE_1; 1.
DR   PROSITE; PS00095; C5_MTASE_2; FALSE_NEG.
DR   PROSITE; PS50812; PWWP; 1.
PE   2: Evidence at transcript level;
KW   Alternative promoter usage; Cytoplasm; DNA-binding; Metal-binding;
KW   Methyltransferase; Nucleus; Phosphoprotein; Repressor;
KW   S-adenosyl-L-methionine; Transcription; Transcription regulation;
KW   Transferase; Zinc; Zinc-finger.
FT   CHAIN         1    908       DNA (cytosine-5)-methyltransferase 3A.
FT                                /FTId=PRO_0000313030.
FT   DOMAIN      257    315       PWWP.
FT   ZN_FING     490    582       ADD-type (By similarity).
FT   REGION      195    399       Interaction with DNMT1 and DNMT3B (By
FT                                similarity).
FT   REGION      490    582       Interaction with the PRC2/EED-EZH2
FT                                complex (By similarity).
FT   COMPBIAS    479    527       Cys-rich.
FT   ACT_SITE    706    706       By similarity.
FT   MOD_RES     102    102       Phosphoserine (By similarity).
FT   VAR_SEQ       1    219       Missing (in isoform 2).
FT                                /FTId=VSP_029986.
SQ   SEQUENCE   908 AA;  101669 MW;  6CCE9EB4E9CEB409 CRC64;
     MPSSGPGDTS ISSLEREDDR KEGEEQEENR GKEERQEPSA TARKVGRPGR KRKHPPVESS
     DTPKDPAVTT KSQPTAQDSG PSDLLPNGDL EKRSEPQPEE GSPAAGQKGG APAEGEGTET
     PPEASRAVEN GCCVTKEGRG ASAGEGKEQK QTNIESMKME GSRGRLRGGL GWESSLRQRP
     MPRLTFQAGD PYYISKRKRD EWLARWKREA EKKAKVIAVM NAVEESQASG ESQKVEEASP
     PAVQQPTDPA SPTVATTPEP VGADAGDKNA TKAADDEPEY EDGRGFGIGE LVWGKLRGFS
     WWPGRIVSWW MTGRSRAAEG TRWVMWFGDG KFSVVCVEKL MPLSSFCSAF HQATYNKQPM
     YRKAIYEVLQ VASSRAGKLF PACHDSDESD TGKAVEVQNK QMIEWALGGF QPSGPKGLEP
     PEEEKNPYKE VYTDMWVEPE AAAYAPPPPA KKPRKSTTEK PKVKEIIDER TRERLVYEVR
     QKCRNIEDIC ISCGSLNVTL EHPLFIGGMC QNCKNCFLEC AYQYDDDGYQ SYCTICCGGR
     EVLMCGNNNC CRCFCVECVD LLVGPGAAQA AIKEDPWNCY MCGHKGTYGL LRRREDWPSR
     LQMFFANNHD QEFDPPKVYP PVPAEKRKPI RVLSLFDGIA TGLLVLKDLG IQVDRYIASE
     VCEDSITVGM VRHQGKIMYV GDVRSVTQKH IQEWGPFDLV IGGSPCNDLS IVNPARKGLY
     EGTGRLFFEF YRLLHDARPK EGDDRPFFWL FENVVAMGVS DKRDISRFLE SNPVMIDAKE
     VSAAHRARYF WGNLPGMNRP LASTVNDKLE LQECLEHGRI AKFSKVRTIT TRSNSIKQGK
     DQHFPVFMNE KEDILWCTEM ERVFGFPVHY TDVSNMSRLA RQRLLGRSWS VPVIRHLFAP
     LKEYFACV
//