Reviewed,
UniProtKB/Swiss-Prot Q1LZ53 (DNM3A_RAT)
Last modified
June 16, 2009.
Version 25.
History...
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: DNA (cytosine-5)-methyltransferase 3A Short name=Dnmt3a EC=2.1.1.37 | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 908 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Required for genome wide de novo methylation and is essential for development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity By similarity. |
| Catalytic activity | S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine. |
| Subunit structure | Interacts with DNMT1 and DNMT3B. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD-type zinc-finger By similarity. Interacts with the PRC2/EED-EZH2 complex By similarity. |
| Subcellular location | |
| Sequence similarities | Belongs to the C5-methyltransferase family. Contains 1 ADD-type zinc finger. Contains 1 PWWP domain. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q1LZ53-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q1LZ53-2) The sequence of this isoform differs from the canonical sequence as follows: 1-219: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 908 | 908 | DNA (cytosine-5)-methyltransferase 3A | PRO_0000313030 | |||||
Regions | |||||||||
| Domain | 257 – 315 | 59 | PWWP | ||||||
| Zinc finger | 490 – 582 | 93 | ADD-type By similarity | ||||||
| Region | 195 – 399 | 205 | Interaction with DNMT1 and DNMT3B By similarity | ||||||
| Region | 490 – 582 | 93 | Interaction with the PRC2/EED-EZH2 complex By similarity | ||||||
| Compositional bias | 479 – 527 | 49 | Cys-rich | ||||||
Sites | |||||||||
| Active site | 706 | 1 | By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 102 | 1 | Phosphoserine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 219 | 219 | Missing in isoform 2. | VSP_029986 | |||||
Sequences
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References
| [1] | "Identification of 11 pseudogenes in the DNA methyltransferase gene family in rodents and humans and implications for the functional loci." Lees-Murdock D.J., McLoughlin G.A., McDaid J.R., Quinn L.M., O'Doherty A., Hiripi L., Hack C.J., Walsh C.P. Genomics 84:193-204(2004) [PubMed: 15203217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). |
Cross-references
Sequence databases | |
|---|---|
| BN000395 mRNA. Translation: CAE52317.1. BN000396 mRNA. Translation: CAE52318.1. | |
| IPI | IPI00387877. IPI00760141. |
| RefSeq | NP_001003957.1. NP_001003958.1. |
| UniGene | Rn.92659 |
3D structure databases | |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | Q1LZ53. |
Genome annotation databases | |
| Ensembl | ENSRNOG00000026649. Rattus norvegicus. [Contig view] |
| GeneID | 444984. |
| KEGG | rno:444984. |
Organism-specific databases | |
| RGD | 1303336. Dnmt3a. |
Phylogenomic databases | |
| HOVERGEN | Q1LZ53. |
Enzyme and pathway databases | |
| BRENDA | 2.1.1.37. 248. |
Gene expression databases | |
| ArrayExpress | Q1LZ53. |
Family and domain databases | |
| InterPro | IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. IPR000313. PWWP. [Graphical view] |
| Pfam | PF00145. DNA_methylase. 1 hit. PF00855. PWWP. 1 hit. [Graphical view] |
| SMART | SM00293. PWWP. 1 hit. [Graphical view] |
| PROSITE | PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. False negative. PS50812. PWWP. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 697058. |
Entry information
| Entry name | DNM3A_RAT | ||||||||
| Accession | Primary (citable) accession number: Q1LZ53 Secondary accession number(s): Q1LZ52 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
