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Protein

DNA (cytosine-5)-methyltransferase 3-like

Gene

Dnmt3l

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context. Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain. Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases (By similarity). Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3 (By similarity). Plays a key role in embryonic stem cells and germ cells. In germ cells, required for the methylation of imprinted loci together with DNMT3A. In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization. Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation. While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes. Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes. Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri87 – 117GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST31
Zinc fingeri128 – 184PHD-type; atypicalPROSITE-ProRule annotationAdd BLAST57

GO - Molecular functioni

GO - Biological processi

  • DNA methylation Source: InterPro
  • response to ethanol Source: RGD

Keywordsi

Biological processDifferentiation, Spermatogenesis
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase 3-like
Gene namesi
Name:Dnmt3l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1303239. Dnmt3l.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002817441 – 422DNA (cytosine-5)-methyltransferase 3-likeAdd BLAST422

Proteomic databases

PaxDbiQ1LZ50.
PRIDEiQ1LZ50.

PTM databases

PhosphoSitePlusiQ1LZ50.

Interactioni

Subunit structurei

Homodimer (By similarity). Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L) (By similarity). Interacts with histone H3 (via N-terminus); interaction is strongly inhibited by methylation at lysine 4 (H3K4me) (By similarity). Interacts with EZH2; the interaction is direct (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001610.

Structurei

3D structure databases

ProteinModelPortaliQ1LZ50.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini76 – 208ADDPROSITE-ProRule annotationAdd BLAST133

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri87 – 117GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST31
Zinc fingeri128 – 184PHD-type; atypicalPROSITE-ProRule annotationAdd BLAST57

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IIXK. Eukaryota.
ENOG4111C55. LUCA.
HOGENOMiHOG000246422.
HOVERGENiHBG051382.
InParanoidiQ1LZ50.
KOiK17400.
PhylomeDBiQ1LZ50.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR025766. ADD.
IPR030486. DNMT3L.
IPR013083. Znf_RING/FYVE/PHD.
PANTHERiPTHR23068:SF15. PTHR23068:SF15. 1 hit.
PROSITEiView protein in PROSITE
PS51533. ADD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1LZ50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSRETPSSC SKTHETLNLE TPESSSTDPD SPLEEQWPKS APDLKEEDSM
60 70 80 90 100
DMVLEDSKEP LTPSSPPTGR EVIRYEVNVN QRNIEDICLC CGSLQVYAQH
110 120 130 140 150
PLFEGGICAP CKDKFLETLF LYDEDGHQSY CTICCSGHTL FICESPDCTR
160 170 180 190 200
CYCFECVDIL VGPGTSERIN AMACWVCFLC LPFSRSGLLQ RRKKWRHQLK
210 220 230 240 250
AFHDREGASP VEIYKTVSAW KRQPVRVLSL FGNIDKELKS LGFLESSSGS
260 270 280 290 300
EGGTLKYVED VTNVVRREVE KWGPFDLVYG STQPLGYSCD RCPGWYMFQF
310 320 330 340 350
HRILQYARPR QDSQQPFFWI FVDNLLLTED DQETTVRFLQ TEAVTLQDVR
360 370 380 390 400
GRVLQNAMRV WSNIPGLKSK HADLTPKEEQ SLQTQVRTRS KLAAQKVDSL
410 420
VKYCLLPLRE YFKYFSQNSL PL
Length:422
Mass (Da):48,279
Last modified:May 30, 2006 - v1
Checksum:iBFA7A78D1E7CBD90
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03116801 Genomic DNA. No translation available.
AABR03116733 Genomic DNA. No translation available.
BN000398 mRNA. Translation: CAE52320.1.
RefSeqiNP_001003964.1. NM_001003964.1.
UniGeneiRn.129905.

Genome annotation databases

GeneIDi309680.
KEGGirno:309680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03116801 Genomic DNA. No translation available.
AABR03116733 Genomic DNA. No translation available.
BN000398 mRNA. Translation: CAE52320.1.
RefSeqiNP_001003964.1. NM_001003964.1.
UniGeneiRn.129905.

3D structure databases

ProteinModelPortaliQ1LZ50.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001610.

PTM databases

PhosphoSitePlusiQ1LZ50.

Proteomic databases

PaxDbiQ1LZ50.
PRIDEiQ1LZ50.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi309680.
KEGGirno:309680.

Organism-specific databases

CTDi29947.
RGDi1303239. Dnmt3l.

Phylogenomic databases

eggNOGiENOG410IIXK. Eukaryota.
ENOG4111C55. LUCA.
HOGENOMiHOG000246422.
HOVERGENiHBG051382.
InParanoidiQ1LZ50.
KOiK17400.
PhylomeDBiQ1LZ50.

Miscellaneous databases

PROiQ1LZ50.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR025766. ADD.
IPR030486. DNMT3L.
IPR013083. Znf_RING/FYVE/PHD.
PANTHERiPTHR23068:SF15. PTHR23068:SF15. 1 hit.
PROSITEiView protein in PROSITE
PS51533. ADD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDNM3L_RAT
AccessioniPrimary (citable) accession number: Q1LZ50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: May 30, 2006
Last modified: February 15, 2017
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.