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Protein

Wee1-like protein kinase 2

Gene

wee2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits cdk1 and acts as a key regulator of meiosis. Required to maintain meiotic arrest in oocytes by phosphorylating cdk1 at 'Tyr-15', leading to inhibit cdk1 activity and prevent meiotic reentry (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei217 – 2171ATPPROSITE-ProRule annotation
Active sitei315 – 3151Proton acceptorPROSITE-ProRule annotation
Metal bindingi320 – 3201Magnesium; via carbonyl oxygenBy similarity
Metal bindingi354 – 3541Magnesium; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi194 – 2029ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  4. protein serine/threonine kinase activity Source: InterPro

GO - Biological processi

  1. meiotic cell cycle Source: UniProtKB-KW
  2. mitotic nuclear division Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Meiosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase 2 (EC:2.7.10.2)
Alternative name(s):
Wee1-like protein kinase 1B
Wee1B kinase
Gene namesi
Name:wee2
Synonyms:wee1b
ORF Names:si:ch211-238n5.5
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Chromosome 18

Organism-specific databases

ZFINiZDB-GENE-030131-5682. wee2.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 532532Wee1-like protein kinase 2PRO_0000409528Add
BLAST

Proteomic databases

PRIDEiQ1LX51.

Expressioni

Gene expression databases

BgeeiQ1LX51.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000040073.

Structurei

3D structure databases

ProteinModelPortaliQ1LX51.
SMRiQ1LX51. Positions 180-460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini188 – 465278Protein kinasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili469 – 49527Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063230.
HOGENOMiHOG000004824.
HOVERGENiHBG005050.
InParanoidiQ1LX51.
KOiK06632.
OMAiLNVEKFK.
PhylomeDBiQ1LX51.
TreeFamiTF101088.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q1LX51-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKWSEMASVN ATQQHLNFSS SWEEDSSDNS FDEWTHKSVP LRSPCRTPRI
60 70 80 90 100
QRHRNRSITV SPSVPTSPIP YAAWKKLRLC DSPSTPKSLL SKSTMPCSSS
110 120 130 140 150
KTCRSQRFLR LSTAFERVPS VNINPFTPDT VRRNSEHYKR KSQRSDDDED
160 170 180 190 200
YGPRSKEIQN SSEDESFFLP SKRPAVSARM LSRYESEFLE LACIGVGEFG
210 220 230 240 250
SVYRCVKRLD GCMYAIKRSR RPIAGSANEQ LALKEVYAHA VLGHHPHVVR
260 270 280 290 300
YYSAWAEDDH MIIQNEYCDG GSLHDAITEK REQGEFFSVP ELRDLLLQVS
310 320 330 340 350
MGLKYIHNSG LVHLDIKPSN IFICRRSTLS AGGEGDSEEE DESHSSGVVY
360 370 380 390 400
KIGDLGHVTS ISSPQVEEGD SRFLAYEVLR EDYTHLPKAD IFALGLTVLL
410 420 430 440 450
AAGASPLPQN GDDWHRLRQG ELPNLPHELP ALFKDLLKSL LDPDPTARPS
460 470 480 490 500
ATALCRHDVL CKERAGKLAT QLRKELNVEK FRTAMLEREL KEARLAATSP
510 520 530
QQSCQPGSLP KAKRKLVGRN VARSVSFGFP GY
Length:532
Mass (Da):59,774
Last modified:May 30, 2011 - v2
Checksum:i2192E4A0F6113D3C
GO
Isoform 2 (identifier: Q1LX51-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: Missing.

Show »
Length:527
Mass (Da):59,113
Checksum:iA3487FAC107A2614
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171N → S in AAI08039 (Ref. 2) Curated
Sequence conflicti37 – 371K → R in AAI34854 (Ref. 2) Curated
Sequence conflicti102 – 1021T → P in AAI08039 (Ref. 2) Curated
Sequence conflicti102 – 1021T → P in AAI34854 (Ref. 2) Curated
Sequence conflicti424 – 4241N → S in AAI08039 (Ref. 2) Curated
Sequence conflicti424 – 4241N → S in AAI34854 (Ref. 2) Curated
Sequence conflicti461 – 4611C → R in AAI08039 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 55Missing in isoform 2. 1 PublicationVSP_041325

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX470166 Genomic DNA. Translation: CAK04661.1.
BX470166 Genomic DNA. Translation: CAK04662.1.
BC108038 mRNA. Translation: AAI08039.1.
BC134853 mRNA. Translation: AAI34854.1.
RefSeqiNP_001032299.2. NM_001037222.2. [Q1LX51-2]
NP_001038367.1. NM_001044902.1. [Q1LX51-1]
UniGeneiDr.6833.

Genome annotation databases

EnsembliENSDART00000040074; ENSDARP00000040073; ENSDARG00000012718. [Q1LX51-2]
ENSDART00000121699; ENSDARP00000109848; ENSDARG00000012718. [Q1LX51-1]
ENSDART00000136710; ENSDARP00000121512; ENSDARG00000012718. [Q1LX51-2]
GeneIDi327471.
KEGGidre:327471.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX470166 Genomic DNA. Translation: CAK04661.1.
BX470166 Genomic DNA. Translation: CAK04662.1.
BC108038 mRNA. Translation: AAI08039.1.
BC134853 mRNA. Translation: AAI34854.1.
RefSeqiNP_001032299.2. NM_001037222.2. [Q1LX51-2]
NP_001038367.1. NM_001044902.1. [Q1LX51-1]
UniGeneiDr.6833.

3D structure databases

ProteinModelPortaliQ1LX51.
SMRiQ1LX51. Positions 180-460.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000040073.

Proteomic databases

PRIDEiQ1LX51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000040074; ENSDARP00000040073; ENSDARG00000012718. [Q1LX51-2]
ENSDART00000121699; ENSDARP00000109848; ENSDARG00000012718. [Q1LX51-1]
ENSDART00000136710; ENSDARP00000121512; ENSDARG00000012718. [Q1LX51-2]
GeneIDi327471.
KEGGidre:327471.

Organism-specific databases

CTDi494551.
ZFINiZDB-GENE-030131-5682. wee2.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063230.
HOGENOMiHOG000004824.
HOVERGENiHBG005050.
InParanoidiQ1LX51.
KOiK06632.
OMAiLNVEKFK.
PhylomeDBiQ1LX51.
TreeFamiTF101088.

Miscellaneous databases

NextBioi20810044.
PROiQ1LX51.

Gene expression databases

BgeeiQ1LX51.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Egg and Ovary.

Entry informationi

Entry nameiWEE2_DANRE
AccessioniPrimary (citable) accession number: Q1LX51
Secondary accession number(s): A4IFZ5, Q1LX50, Q32PP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2011
Last sequence update: May 30, 2011
Last modified: January 6, 2015
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.