Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q1LX51 (WEE2_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Wee1-like protein kinase 2

EC=2.7.10.2
Alternative name(s):
Wee1-like protein kinase 1B
Wee1B kinase
Gene names
Name:wee2
Synonyms:wee1b
ORF Names:si:ch211-238n5.5
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length532 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits cdk1 and acts as a key regulator of meiosis. Required to maintain meiotic arrest in oocytes by phosphorylating cdk1 at 'Tyr-15', leading to inhibit cdk1 activity and prevent meiotic reentry By similarity.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q1LX51-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q1LX51-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 532532Wee1-like protein kinase 2
PRO_0000409528

Regions

Domain188 – 465278Protein kinase
Nucleotide binding194 – 2029ATP By similarity
Coiled coil469 – 49527 Potential

Sites

Active site3151Proton acceptor By similarity
Metal binding3201Magnesium; via carbonyl oxygen By similarity
Metal binding3541Magnesium; via carbonyl oxygen By similarity
Binding site2171ATP By similarity

Natural variations

Alternative sequence1 – 55Missing in isoform 2.
VSP_041325

Experimental info

Sequence conflict171N → S in AAI08039. Ref.2
Sequence conflict371K → R in AAI34854. Ref.2
Sequence conflict1021T → P in AAI08039. Ref.2
Sequence conflict1021T → P in AAI34854. Ref.2
Sequence conflict4241N → S in AAI08039. Ref.2
Sequence conflict4241N → S in AAI34854. Ref.2
Sequence conflict4611C → R in AAI08039. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 31, 2011. Version 2.
Checksum: 2192E4A0F6113D3C

FASTA53259,774
        10         20         30         40         50         60 
MKWSEMASVN ATQQHLNFSS SWEEDSSDNS FDEWTHKSVP LRSPCRTPRI QRHRNRSITV 

        70         80         90        100        110        120 
SPSVPTSPIP YAAWKKLRLC DSPSTPKSLL SKSTMPCSSS KTCRSQRFLR LSTAFERVPS 

       130        140        150        160        170        180 
VNINPFTPDT VRRNSEHYKR KSQRSDDDED YGPRSKEIQN SSEDESFFLP SKRPAVSARM 

       190        200        210        220        230        240 
LSRYESEFLE LACIGVGEFG SVYRCVKRLD GCMYAIKRSR RPIAGSANEQ LALKEVYAHA 

       250        260        270        280        290        300 
VLGHHPHVVR YYSAWAEDDH MIIQNEYCDG GSLHDAITEK REQGEFFSVP ELRDLLLQVS 

       310        320        330        340        350        360 
MGLKYIHNSG LVHLDIKPSN IFICRRSTLS AGGEGDSEEE DESHSSGVVY KIGDLGHVTS 

       370        380        390        400        410        420 
ISSPQVEEGD SRFLAYEVLR EDYTHLPKAD IFALGLTVLL AAGASPLPQN GDDWHRLRQG 

       430        440        450        460        470        480 
ELPNLPHELP ALFKDLLKSL LDPDPTARPS ATALCRHDVL CKERAGKLAT QLRKELNVEK 

       490        500        510        520        530 
FRTAMLEREL KEARLAATSP QQSCQPGSLP KAKRKLVGRN VARSVSFGFP GY 

« Hide

Isoform 2 [UniParc].

Checksum: A3487FAC107A2614
Show »

FASTA52759,113

References

[1]"The zebrafish reference genome sequence and its relationship to the human genome."
Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J. expand/collapse author list , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tuebingen.
[2]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Egg and Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX470166 Genomic DNA. Translation: CAK04661.1.
BX470166 Genomic DNA. Translation: CAK04662.1.
BC108038 mRNA. Translation: AAI08039.1.
BC134853 mRNA. Translation: AAI34854.1.
RefSeqNP_001032299.2. NM_001037222.2. [Q1LX51-2]
NP_001038367.1. NM_001044902.1. [Q1LX51-1]
UniGeneDr.6833.

3D structure databases

ProteinModelPortalQ1LX51.
SMRQ1LX51. Positions 180-460.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000040073.

Proteomic databases

PRIDEQ1LX51.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000040074; ENSDARP00000040073; ENSDARG00000012718. [Q1LX51-2]
ENSDART00000121699; ENSDARP00000109848; ENSDARG00000012718. [Q1LX51-1]
ENSDART00000136710; ENSDARP00000121512; ENSDARG00000012718. [Q1LX51-2]
GeneID327471.
KEGGdre:327471.

Organism-specific databases

CTD494551.
ZFINZDB-GENE-030131-5682. wee2.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00530000063230.
HOGENOMHOG000004824.
HOVERGENHBG005050.
InParanoidQ1LX51.
KOK06632.
OMAEFLELEC.
PhylomeDBQ1LX51.
TreeFamTF101088.

Gene expression databases

BgeeQ1LX51.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20810044.
PROQ1LX51.

Entry information

Entry nameWEE2_DANRE
AccessionPrimary (citable) accession number: Q1LX51
Secondary accession number(s): A4IFZ5, Q1LX50, Q32PP5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: May 31, 2011
Last modified: July 9, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families