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Q1LX51

- WEE2_DANRE

UniProt

Q1LX51 - WEE2_DANRE

Protein

Wee1-like protein kinase 2

Gene

wee2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 2 (31 May 2011)
      Previous versions | rss
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    Functioni

    Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits cdk1 and acts as a key regulator of meiosis. Required to maintain meiotic arrest in oocytes by phosphorylating cdk1 at 'Tyr-15', leading to inhibit cdk1 activity and prevent meiotic reentry By similarity.By similarity

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei217 – 2171ATPPROSITE-ProRule annotation
    Active sitei315 – 3151Proton acceptorPROSITE-ProRule annotation
    Metal bindingi320 – 3201Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi354 – 3541Magnesium; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi194 – 2029ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    4. protein serine/threonine kinase activity Source: InterPro

    GO - Biological processi

    1. meiotic nuclear division Source: UniProtKB-KW
    2. mitotic nuclear division Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Meiosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Wee1-like protein kinase 2 (EC:2.7.10.2)
    Alternative name(s):
    Wee1-like protein kinase 1B
    Wee1B kinase
    Gene namesi
    Name:wee2
    Synonyms:wee1b
    ORF Names:si:ch211-238n5.5
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 18

    Organism-specific databases

    ZFINiZDB-GENE-030131-5682. wee2.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 532532Wee1-like protein kinase 2PRO_0000409528Add
    BLAST

    Proteomic databases

    PRIDEiQ1LX51.

    Expressioni

    Gene expression databases

    BgeeiQ1LX51.

    Interactioni

    Protein-protein interaction databases

    STRINGi7955.ENSDARP00000040073.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1LX51.
    SMRiQ1LX51. Positions 180-460.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini188 – 465278Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili469 – 49527Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00530000063230.
    HOGENOMiHOG000004824.
    HOVERGENiHBG005050.
    InParanoidiQ1LX51.
    KOiK06632.
    OMAiEFLELEC.
    PhylomeDBiQ1LX51.
    TreeFamiTF101088.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    IPR017164. Wee1-like_protein_kinase.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q1LX51-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKWSEMASVN ATQQHLNFSS SWEEDSSDNS FDEWTHKSVP LRSPCRTPRI    50
    QRHRNRSITV SPSVPTSPIP YAAWKKLRLC DSPSTPKSLL SKSTMPCSSS 100
    KTCRSQRFLR LSTAFERVPS VNINPFTPDT VRRNSEHYKR KSQRSDDDED 150
    YGPRSKEIQN SSEDESFFLP SKRPAVSARM LSRYESEFLE LACIGVGEFG 200
    SVYRCVKRLD GCMYAIKRSR RPIAGSANEQ LALKEVYAHA VLGHHPHVVR 250
    YYSAWAEDDH MIIQNEYCDG GSLHDAITEK REQGEFFSVP ELRDLLLQVS 300
    MGLKYIHNSG LVHLDIKPSN IFICRRSTLS AGGEGDSEEE DESHSSGVVY 350
    KIGDLGHVTS ISSPQVEEGD SRFLAYEVLR EDYTHLPKAD IFALGLTVLL 400
    AAGASPLPQN GDDWHRLRQG ELPNLPHELP ALFKDLLKSL LDPDPTARPS 450
    ATALCRHDVL CKERAGKLAT QLRKELNVEK FRTAMLEREL KEARLAATSP 500
    QQSCQPGSLP KAKRKLVGRN VARSVSFGFP GY 532
    Length:532
    Mass (Da):59,774
    Last modified:May 31, 2011 - v2
    Checksum:i2192E4A0F6113D3C
    GO
    Isoform 2 (identifier: Q1LX51-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-5: Missing.

    Show »
    Length:527
    Mass (Da):59,113
    Checksum:iA3487FAC107A2614
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171N → S in AAI08039. 1 PublicationCurated
    Sequence conflicti37 – 371K → R in AAI34854. 1 PublicationCurated
    Sequence conflicti102 – 1021T → P in AAI08039. 1 PublicationCurated
    Sequence conflicti102 – 1021T → P in AAI34854. 1 PublicationCurated
    Sequence conflicti424 – 4241N → S in AAI08039. 1 PublicationCurated
    Sequence conflicti424 – 4241N → S in AAI34854. 1 PublicationCurated
    Sequence conflicti461 – 4611C → R in AAI08039. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 55Missing in isoform 2. 1 PublicationVSP_041325

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX470166 Genomic DNA. Translation: CAK04661.1.
    BX470166 Genomic DNA. Translation: CAK04662.1.
    BC108038 mRNA. Translation: AAI08039.1.
    BC134853 mRNA. Translation: AAI34854.1.
    RefSeqiNP_001032299.2. NM_001037222.2. [Q1LX51-2]
    NP_001038367.1. NM_001044902.1. [Q1LX51-1]
    UniGeneiDr.6833.

    Genome annotation databases

    EnsembliENSDART00000040074; ENSDARP00000040073; ENSDARG00000012718. [Q1LX51-2]
    ENSDART00000121699; ENSDARP00000109848; ENSDARG00000012718. [Q1LX51-1]
    ENSDART00000136710; ENSDARP00000121512; ENSDARG00000012718. [Q1LX51-2]
    GeneIDi327471.
    KEGGidre:327471.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX470166 Genomic DNA. Translation: CAK04661.1 .
    BX470166 Genomic DNA. Translation: CAK04662.1 .
    BC108038 mRNA. Translation: AAI08039.1 .
    BC134853 mRNA. Translation: AAI34854.1 .
    RefSeqi NP_001032299.2. NM_001037222.2. [Q1LX51-2 ]
    NP_001038367.1. NM_001044902.1. [Q1LX51-1 ]
    UniGenei Dr.6833.

    3D structure databases

    ProteinModelPortali Q1LX51.
    SMRi Q1LX51. Positions 180-460.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7955.ENSDARP00000040073.

    Proteomic databases

    PRIDEi Q1LX51.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000040074 ; ENSDARP00000040073 ; ENSDARG00000012718 . [Q1LX51-2 ]
    ENSDART00000121699 ; ENSDARP00000109848 ; ENSDARG00000012718 . [Q1LX51-1 ]
    ENSDART00000136710 ; ENSDARP00000121512 ; ENSDARG00000012718 . [Q1LX51-2 ]
    GeneIDi 327471.
    KEGGi dre:327471.

    Organism-specific databases

    CTDi 494551.
    ZFINi ZDB-GENE-030131-5682. wee2.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00530000063230.
    HOGENOMi HOG000004824.
    HOVERGENi HBG005050.
    InParanoidi Q1LX51.
    KOi K06632.
    OMAi EFLELEC.
    PhylomeDBi Q1LX51.
    TreeFami TF101088.

    Miscellaneous databases

    NextBioi 20810044.
    PROi Q1LX51.

    Gene expression databases

    Bgeei Q1LX51.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    IPR017164. Wee1-like_protein_kinase.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037281. Wee1-like_protein_kinase. 1 hit.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The zebrafish reference genome sequence and its relationship to the human genome."
      Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
      , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
      Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tuebingen.
    2. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Egg and Ovary.

    Entry informationi

    Entry nameiWEE2_DANRE
    AccessioniPrimary (citable) accession number: Q1LX51
    Secondary accession number(s): A4IFZ5, Q1LX50, Q32PP5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 31, 2011
    Last sequence update: May 31, 2011
    Last modified: October 1, 2014
    This is version 71 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3