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Q1LX51

- WEE2_DANRE

UniProt

Q1LX51 - WEE2_DANRE

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Protein

Wee1-like protein kinase 2

Gene
wee2, wee1b, si:ch211-238n5.5
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits cdk1 and acts as a key regulator of meiosis. Required to maintain meiotic arrest in oocytes by phosphorylating cdk1 at 'Tyr-15', leading to inhibit cdk1 activity and prevent meiotic reentry By similarity.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei217 – 2171ATP By similarity
Active sitei315 – 3151Proton acceptor By similarity
Metal bindingi320 – 3201Magnesium; via carbonyl oxygen By similarity
Metal bindingi354 – 3541Magnesium; via carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi194 – 2029ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  4. protein serine/threonine kinase activity Source: InterPro

GO - Biological processi

  1. meiotic nuclear division Source: UniProtKB-KW
  2. mitotic nuclear division Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Meiosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase 2 (EC:2.7.10.2)
Alternative name(s):
Wee1-like protein kinase 1B
Wee1B kinase
Gene namesi
Name:wee2
Synonyms:wee1b
ORF Names:si:ch211-238n5.5
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 18

Organism-specific databases

ZFINiZDB-GENE-030131-5682. wee2.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 532532Wee1-like protein kinase 2PRO_0000409528Add
BLAST

Proteomic databases

PRIDEiQ1LX51.

Expressioni

Gene expression databases

BgeeiQ1LX51.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000040073.

Structurei

3D structure databases

ProteinModelPortaliQ1LX51.
SMRiQ1LX51. Positions 180-460.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini188 – 465278Protein kinaseAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili469 – 49527 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063230.
HOGENOMiHOG000004824.
HOVERGENiHBG005050.
InParanoidiQ1LX51.
KOiK06632.
OMAiEFLELEC.
PhylomeDBiQ1LX51.
TreeFamiTF101088.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q1LX51-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKWSEMASVN ATQQHLNFSS SWEEDSSDNS FDEWTHKSVP LRSPCRTPRI    50
QRHRNRSITV SPSVPTSPIP YAAWKKLRLC DSPSTPKSLL SKSTMPCSSS 100
KTCRSQRFLR LSTAFERVPS VNINPFTPDT VRRNSEHYKR KSQRSDDDED 150
YGPRSKEIQN SSEDESFFLP SKRPAVSARM LSRYESEFLE LACIGVGEFG 200
SVYRCVKRLD GCMYAIKRSR RPIAGSANEQ LALKEVYAHA VLGHHPHVVR 250
YYSAWAEDDH MIIQNEYCDG GSLHDAITEK REQGEFFSVP ELRDLLLQVS 300
MGLKYIHNSG LVHLDIKPSN IFICRRSTLS AGGEGDSEEE DESHSSGVVY 350
KIGDLGHVTS ISSPQVEEGD SRFLAYEVLR EDYTHLPKAD IFALGLTVLL 400
AAGASPLPQN GDDWHRLRQG ELPNLPHELP ALFKDLLKSL LDPDPTARPS 450
ATALCRHDVL CKERAGKLAT QLRKELNVEK FRTAMLEREL KEARLAATSP 500
QQSCQPGSLP KAKRKLVGRN VARSVSFGFP GY 532
Length:532
Mass (Da):59,774
Last modified:May 31, 2011 - v2
Checksum:i2192E4A0F6113D3C
GO
Isoform 2 (identifier: Q1LX51-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: Missing.

Show »
Length:527
Mass (Da):59,113
Checksum:iA3487FAC107A2614
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 55Missing in isoform 2. VSP_041325

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171N → S in AAI08039. 1 Publication
Sequence conflicti37 – 371K → R in AAI34854. 1 Publication
Sequence conflicti102 – 1021T → P in AAI08039. 1 Publication
Sequence conflicti102 – 1021T → P in AAI34854. 1 Publication
Sequence conflicti424 – 4241N → S in AAI08039. 1 Publication
Sequence conflicti424 – 4241N → S in AAI34854. 1 Publication
Sequence conflicti461 – 4611C → R in AAI08039. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX470166 Genomic DNA. Translation: CAK04661.1.
BX470166 Genomic DNA. Translation: CAK04662.1.
BC108038 mRNA. Translation: AAI08039.1.
BC134853 mRNA. Translation: AAI34854.1.
RefSeqiNP_001032299.2. NM_001037222.2. [Q1LX51-2]
NP_001038367.1. NM_001044902.1. [Q1LX51-1]
UniGeneiDr.6833.

Genome annotation databases

EnsembliENSDART00000040074; ENSDARP00000040073; ENSDARG00000012718. [Q1LX51-2]
ENSDART00000121699; ENSDARP00000109848; ENSDARG00000012718. [Q1LX51-1]
ENSDART00000136710; ENSDARP00000121512; ENSDARG00000012718. [Q1LX51-2]
GeneIDi327471.
KEGGidre:327471.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX470166 Genomic DNA. Translation: CAK04661.1 .
BX470166 Genomic DNA. Translation: CAK04662.1 .
BC108038 mRNA. Translation: AAI08039.1 .
BC134853 mRNA. Translation: AAI34854.1 .
RefSeqi NP_001032299.2. NM_001037222.2. [Q1LX51-2 ]
NP_001038367.1. NM_001044902.1. [Q1LX51-1 ]
UniGenei Dr.6833.

3D structure databases

ProteinModelPortali Q1LX51.
SMRi Q1LX51. Positions 180-460.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7955.ENSDARP00000040073.

Proteomic databases

PRIDEi Q1LX51.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000040074 ; ENSDARP00000040073 ; ENSDARG00000012718 . [Q1LX51-2 ]
ENSDART00000121699 ; ENSDARP00000109848 ; ENSDARG00000012718 . [Q1LX51-1 ]
ENSDART00000136710 ; ENSDARP00000121512 ; ENSDARG00000012718 . [Q1LX51-2 ]
GeneIDi 327471.
KEGGi dre:327471.

Organism-specific databases

CTDi 494551.
ZFINi ZDB-GENE-030131-5682. wee2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00530000063230.
HOGENOMi HOG000004824.
HOVERGENi HBG005050.
InParanoidi Q1LX51.
KOi K06632.
OMAi EFLELEC.
PhylomeDBi Q1LX51.
TreeFami TF101088.

Miscellaneous databases

NextBioi 20810044.
PROi Q1LX51.

Gene expression databases

Bgeei Q1LX51.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Egg and Ovary.

Entry informationi

Entry nameiWEE2_DANRE
AccessioniPrimary (citable) accession number: Q1LX51
Secondary accession number(s): A4IFZ5, Q1LX50, Q32PP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: May 31, 2011
Last modified: September 3, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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