Reviewed, UniProtKB/Swiss-Prot Q1LWG4 (PCAT1_DANRE)

Names and origin

Protein names

Recommended name:
    Lysophosphatidylcholine acyltransferase 1
      Short name=LysoPC acyltransferase 1
      Short name=LPC acyltransferase 1
      Short name=LPCAT-1
    EC=2.3.1.-
Alternative name(s):
    1-alkylglycerophosphocholine O-acetyltransferase
    EC=2.3.1.67
    Acetyl-CoA:lyso-platelet-activating factor acetyltransferase
      Short name=Acetyl-CoA:lyso-PAF acetyltransferase
      Short name=Lyso-PAF acetyltransferase
      Short name=LysoPAFAT
    1-acylglycerophosphocholine O-acyltransferase
    EC=2.3.1.23
    Acyltransferase-like 2

Gene names

Name:	lpcat1
Synonyms:	aytl2
ORF Names:	si:dkey-261i16.4, zgc:158232

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC) By similarity.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine = CoA + 1,2-diacyl-sn-glycero-3-phosphocholine. Acetyl-CoA + 1-alkyl-sn-glycero-3-phosphocholine = CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine.
Pathway	Lipid metabolism; phospholipid metabolism.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphocholine By similarity. The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. Contains 1 EF-hand domain.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Repeat Signal-anchor Transmembrane
Ligand	Calcium
Molecular function	Acyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	Golgi apparatus Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-acylglycerophosphocholine O-acyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB 1-alkylglycerophosphocholine O-acetyltransferase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	Lysophosphatidylcholine acyltransferase 1		PRO_0000247067
Regions
	Topological domain	1 – 52	52	Cytoplasmic Potential
	Transmembrane	53 – 73	21	Signal-anchor for type II membrane protein Potential
	Topological domain	74 – 517	444	Lumenal Potential
	Domain	443 – 478	36	EF-hand
	Motif	129 – 134	6	HXXXXD motif
	Motif	514 – 517	4	Di-lysine motif
Amino acid modifications
	Glycosylation	207	1	N-linked (GlcNAc...) Potential
	Glycosylation	511	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	347	1	Q → R in AAI29168. Ref.2
	Sequence conflict	351	1	C → S in AAI29168. Ref.2
	Sequence conflict	467	1	E → D in AAI29168. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q1LWG4-1 [UniParc]. Last modified May 30, 2006. Version 1. Checksum: DA4A17F30B6D39CD	FASTA	517	58,727
	10 20 30 40 50 60 MRFPNRKLHT AVNGGDSGVS THFRNPFVHE LRFTTLQKLK IAVMTVTLFP VRLLFAAFMM 70 80 90 100 110 120 LLAWPFAFVA TVGRSENAVE PLSWWRWLVD LALKAIMRAM WFSGGFHWVR VKGRPALPSE 130 140 150 160 170 180 APILTMAPHS SYFDAIPVTM TMASIVMKAE SKDIPVWGTL IKFIRPVFVS RSDQDSRRKT 190 200 210 220 230 240 VEEIKRRASS NGEWPQIMIF PEGTCTNRSC LIAFKPGAFI PGVPVQPVVL RYPNELDTIS 250 260 270 280 290 300 WTWQGPGAFK ILWLTLCQLH NFVEIEYLPT YTPSEEEKKD PALFASNVRR IMAKALGLPI 310 320 330 340 350 360 IDYSFEDCQL AMAKGPLRLP KHTCLLEFAR LVRLLGLKTK VTDEVLQEEA CSARQLCGRR 370 380 390 400 410 420 LDMEGFAQYL HQPMTEAVQD IFSLFEEHGM MDVREYVIAL SVVCRPFRYL DTVKLAFRMF 430 440 450 460 470 480 EAQEDGAIVE DELTVILKTA LGVGDLAVSE LFRAIDSQDK GKITFDELCS FMEKCPDLVE 490 500 510 QYHCLCESIS QHSRESTSSS NGFCADFSPR NHSKKQD

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571830 Genomic DNA. Translation: CAK10868.1. BC129167 mRNA. Translation: AAI29168.1.
IPI	IPI00497621.
RefSeq	NP_001037806.2.
UniGene	Dr.80588
3D structure databases
SMR	Q1LWG4. Positions 360-481.
ModBase	Search...
Genome annotation databases
Ensembl	ENSDART00000103855; ENSDARP00000094631; ENSDARG00000011506; Danio rerio. [Genome view]
GeneID	555969.
KEGG	dre:555969.
Organism-specific databases
CTD	555969.
ZFIN	ZDB-GENE-060503-915. lpcat1.
Phylogenomic databases
eggNOG	fiNOG11461.
HOGENOM	HBG446767.
HOVERGEN	Q1LWG4.
InParanoid	Q1LWG4.
OMA	RAQSNGK.
OrthoDB	EOG9577ST.
Enzyme and pathway databases
BRENDA	2.3.1.23. 96826.
Gene expression databases
ArrayExpress	Q1LWG4.
Bgee	Q1LWG4.
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR011992. EF-hand-like_dom. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00054. EFh. 2 hits. SM00563. PlsC. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. False negative. PS50222. EF_HAND_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCAT1_DANRE

Accession

Primary (citable) accession number: Q1LWG4
Secondary accession number(s): A1L1Q0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	May 30, 2006
Last modified:	January 19, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project