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Protein

ALX homeobox protein 1

Gene

alx1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Sequence-specific DNA-binding transcription factor that binds palindromic sequences within promoters and may activate or repress the transcription of a subset of genes. Most probably regulates the expression of genes involved in the development of mesenchyme-derived craniofacial structures (By similarity). Required for proper neural crest cell migration into the frontonasal primordia during development (PubMed:23059813).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi120 – 179HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

  • embryonic cranial skeleton morphogenesis Source: ZFIN
  • neural crest cell migration Source: ZFIN
  • positive regulation of epithelial to mesenchymal transition Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ALX homeobox protein 1Curated
Alternative name(s):
Cartilage homeoprotein 1By similarity
Short name:
CART-1By similarity
Gene namesi
Name:alx1By similarity
Synonyms:cart1By similarity
ORF Names:si:ch211-154h20.1Imported, si:dkey-66c4.1Imported
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 18

Organism-specific databases

ZFINiZDB-GENE-050419-191. alx1.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Morpholino-mediated knockdown causes severe craniofacial alterations including small, misshapen head, microphthalmia and loss of the jaw. It is associated with a more or less severe loss of the cartilaginous facial skeleton. Pericardial edema is also detected.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004330211 – 320ALX homeobox protein 1Add BLAST320

Proteomic databases

PaxDbiQ1LVQ7.
PRIDEiQ1LVQ7.

Expressioni

Developmental stagei

Expression is initially detected at the tailbud stage, immediately after gastrulation, in the cephalic mesoderm. It is maintained in the mesoderm during early stages of development. Expression is initiated in the neural crest by the 5 somites stage, and is restricted to a rostral domain of cranial neural crest. Later during development it is still expressed in specific regions of the neural crest-derived facial mesenchyme. In 24 hpf stage embryos, expressed strongly in the periocular mesenchyme. By 48 hpf, expression is maintained in the periocular mesenchyme towards the anterior of the head, with weaker expression in the nasal region and the prospective palate. Also expressed in a discrete region at the distal tip of the mandible arch and in the pectoral fin bud primordia.1 Publication

Gene expression databases

BgeeiENSDARG00000062824.

Interactioni

Subunit structurei

Binds DNA as a homodimer; required for transcriptional activation.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000085677.

Structurei

3D structure databases

ProteinModelPortaliQ1LVQ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni180 – 320Transactivation domainBy similarityAdd BLAST141

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi300 – 313OARPROSITE-ProRule annotationAdd BLAST14

Domaini

The OAR motif may negatively regulate DNA-binding and therefore transcriptional activity. It is found in the C-terminal transactivation domain that stimulates transcription.By similarity

Sequence similaritiesi

Belongs to the paired homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00760000118958.
HOGENOMiHOG000231518.
HOVERGENiHBG050798.
KOiK09334.
OMAiHVSLNNF.
OrthoDBiEOG091G0B1L.
PhylomeDBiQ1LVQ7.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR033209. ALX1.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PANTHERiPTHR24329:SF359. PTHR24329:SF359. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1LVQ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYLSDKFSL KSPAIKGSDY YMDQVMDTLD NVQYYNKASP KCVQAFPMQS
60 70 80 90 100
NDQHSSMDRS SPCDNQSSVT YCAPKSEESS LHAMENCCSL RVSPATSGPD
110 120 130 140 150
KTDLDELGEK CDSNVSSSKK RRHRTTFTSA QLEELEKVFQ KTHYPDVYVR
160 170 180 190 200
EQLAMRTELT EARVQVWFQN RRAKWRKRER YGQIQQAKSH FAATYDISML
210 220 230 240 250
PRTDSYSQIS NNLWTGPSAG SSVVSSCMIP RGSPPCVTSP YPHSPRAAEH
260 270 280 290 300
GYVGFPNHQQ NQFGVNHVSL NNFFADSLLA SSANSHAAFE TKPEFERRSS
310 320
SIAVLRMKAK EHTANISWAM
Length:320
Mass (Da):36,131
Last modified:February 20, 2007 - v1
Checksum:i38C1017AAE17BC26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX000347 Genomic DNA. Translation: CAK11128.1.
BX649507 Genomic DNA. No translation available.
RefSeqiNP_001038539.1. NM_001045074.1.
UniGeneiDr.141993.

Genome annotation databases

EnsembliENSDART00000142584; ENSDARP00000123391; ENSDARG00000062824.
GeneIDi565176.
KEGGidre:565176.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX000347 Genomic DNA. Translation: CAK11128.1.
BX649507 Genomic DNA. No translation available.
RefSeqiNP_001038539.1. NM_001045074.1.
UniGeneiDr.141993.

3D structure databases

ProteinModelPortaliQ1LVQ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000085677.

Proteomic databases

PaxDbiQ1LVQ7.
PRIDEiQ1LVQ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000142584; ENSDARP00000123391; ENSDARG00000062824.
GeneIDi565176.
KEGGidre:565176.

Organism-specific databases

CTDi8092.
ZFINiZDB-GENE-050419-191. alx1.

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00760000118958.
HOGENOMiHOG000231518.
HOVERGENiHBG050798.
KOiK09334.
OMAiHVSLNNF.
OrthoDBiEOG091G0B1L.
PhylomeDBiQ1LVQ7.

Miscellaneous databases

PROiQ1LVQ7.

Gene expression databases

BgeeiENSDARG00000062824.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR033209. ALX1.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PANTHERiPTHR24329:SF359. PTHR24329:SF359. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALX1_DANRE
AccessioniPrimary (citable) accession number: Q1LVQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2015
Last sequence update: February 20, 2007
Last modified: October 5, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.